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Literature summary extracted from

  • Petzelbauer, I.; Kuhn, B.; Splechtna, B.; Kulbe, K.D.; Nidetzky, B.
    Development of an ultrahigh-temperature process for the enzymatic hydrolysis of lactose. IV. Immobilization of two thermostable beta-glycosidases and optimization of a packed-bed reactor for lactose conversion (2002), Biotechnol. Bioeng., 77, 619-631.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.B26 food industry the immobilized enzyme is useful for the hydrolysis of lactose in whey or milk by using a packed-bed enzyme reactor operated at 70°C Saccharolobus solfataricus
3.2.1.B26 synthesis the immobilized enzyme is useful for the production of galactooligosaccharides by using a packed-bed enzyme reactor operated at 70°C Saccharolobus solfataricus
3.2.1.B28 food industry the immobilized enzyme is useful for the hydrolysis of lactose in whey or milk by using a packed-bed enzyme reactor operated at 70°C Pyrococcus furiosus
3.2.1.B28 synthesis the immobilized enzyme is useful for the production of galactooligosaccharides by using a packed-bed enzyme reactor operated at 70°C Pyrococcus furiosus

General Stability

EC Number General Stability Organism
3.2.1.B26 the enzyme can be immobilized with little loss of enzyme activity and catalytic efficiency by covalent coupling of the proteins via the surface amino groups to insoluble carriers Saccharolobus solfataricus
3.2.1.B28 the enzyme can be immobilized with little loss of enzyme activity and catalytic efficiency by covalent coupling of the proteins via the surface amino groups to insoluble carriers Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.B26 44
-
 lactose pH 5.5, 70°C, enzyme immobilized on Eupergit C Saccharolobus solfataricus
3.2.1.B26 63
-
 lactose pH 5.5, 70°C, soluble enzyme Saccharolobus solfataricus
3.2.1.B26 70
-
 lactose pH 5.5, 70°C, enzyme immobilized on chitosan Saccharolobus solfataricus
3.2.1.B26 131
-
 lactose pH 5.5, 70°C, enzyme immobilized on controlled pore glass Saccharolobus solfataricus
3.2.1.B28 49
-
 lactose pH 5.5, 70°C, enzyme immobilized on Eupergit C Pyrococcus furiosus
3.2.1.B28 61
-
 lactose pH 5.5, 70°C, soluble enzyme Pyrococcus furiosus
3.2.1.B28 62
-
 lactose pH 5.5, 70°C, enzyme immobilized on chitosan Pyrococcus furiosus
3.2.1.B28 202
-
 lactose pH 5.5, 70°C, enzyme immobilized on controlled pore glass Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B26 Saccharolobus solfataricus
-
-
-
3.2.1.B28 Pyrococcus furiosus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B26 lactose + H2O
-
 Saccharolobus solfataricus D-glucose + D-galactose
-
 ?
3.2.1.B28 lactose + H2O
-
 Pyrococcus furiosus D-glucose + D-galactose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.B26 SsbetaGly
-
 Saccharolobus solfataricus
3.2.1.B28 CelB
-
 Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.B26 70
-
 pH 5.5, immobilized enzyme, half-life: 10 days Saccharolobus solfataricus
3.2.1.B28 70
-
 pH 5.5, immobilized enzyme, half-life: 60 days Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B26 5 6 soluble enzyme and immobilized enzymes Saccharolobus solfataricus
3.2.1.B28 5 6 soluble enzyme and immobilized enzymes Pyrococcus furiosus