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Literature summary extracted from

  • Petzelbauer, I.; Splechtna, B.; Nidetzky, B.
    Development of an ultrahigh-temperature process for the enzymatic hydrolysis of lactose. III. Utilization of two thermostable beta-glycosidases in a continuous ultrafiltration membrane reactor and galacto-oligosaccharide formation under steady-state conditions (2002), Biotechnol. Bioeng., 77, 394-404.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.B26 synthesis a continuous stirred-tank reactor charged with the enzyme and operated at steady-state conditions could be a useful reaction system for the production of galacto-oligosaccharides in which composition is narrower and more easily programmable, in terms of the individual components contained, as compared to the batchwise reaction Saccharolobus solfataricus
3.2.1.B28 synthesis a continuous stirred-tank reactor charged with the enzyme and operated at steady-state conditions could be a useful reaction system for the production of galacto-oligosaccharides in which composition is narrower and more easily programmable, in terms of the individual components contained, as compared to the batchwise reaction Pyrococcus furiosus

General Stability

EC Number General Stability Organism
3.2.1.B26 half-life times of the enzyme in hydrolysis of lactose is carried out at 70°C in a continuous stirred-tank reactor coupled to a 10000 Da cross-flow ultrafiltration module (to recycle the enzyme) is approximately 5 to 7 days Saccharolobus solfataricus
3.2.1.B28 half-life times of the enzyme in hydrolysis of lactose is carried out at 70°C in a continuous stirred-tank reactor coupled to a 10000 Da cross-flow ultrafiltration module (to recycle the enzyme) is approximately 5 to 7 days Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B26 Saccharolobus solfataricus
-
-
-
3.2.1.B28 Pyrococcus furiosus Q51723
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B26 lactose + H2O
-
 Saccharolobus solfataricus D-glucose + D-galactose
-
 ?
3.2.1.B28 lactose + H2O
-
 Pyrococcus furiosus D-glucose + D-galactose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.B26 SsbetaGly
-
 Saccharolobus solfataricus
3.2.1.B28 CelB
-
 Pyrococcus furiosus