EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.3.4 | 2,2'-bipyridyl | 50% inhibition at 1 mM | Narcissus tazetta | |
1.4.3.4 | 2-Bromoethylamine | complete inhibition at 0.01 mM | Narcissus tazetta | |
1.4.3.4 | additional information | hardly inhibited by 1 mM o-phenanthroline (4%), pargyline (2%) and clorgyline (1%) | Narcissus tazetta | |
1.4.3.4 | Semicarbazide | complete inhibition at 0.01 mM | Narcissus tazetta |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.4 | 0.059 | - |
n-Propylamine | at pH 7.0 and 37°C | Narcissus tazetta | |
1.4.3.4 | 0.11 | - |
methylamine | at pH 7.0 and 37°C | Narcissus tazetta | |
1.4.3.4 | 0.11 | - |
n-butylamine | at pH 7.0 and 37°C | Narcissus tazetta | |
1.4.3.4 | 0.13 | - |
ethylamine | at pH 7.0 and 37°C | Narcissus tazetta | |
1.4.3.4 | 0.41 | - |
benzylamine | at pH 7.0 and 37°C | Narcissus tazetta | |
1.4.3.4 | 0.5 | - |
n-Pentylamine | at pH 7.0 and 37°C | Narcissus tazetta | |
1.4.3.4 | 1.4 | - |
2-Phenylethylamine | at pH 7.0 and 37°C | Narcissus tazetta | |
1.4.3.4 | 2 | - |
ethanolamine | at pH 7.0 and 37°C | Narcissus tazetta |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.4.3.4 | Cu2+ | the enzyme contains 2.44 mol of copper atoms per mole of enzyme | Narcissus tazetta | |
1.4.3.4 | Mn2+ | 133% activity at 0.1 mM Mn2+ | Narcissus tazetta |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.4.3.4 | 65000 | - |
1 * 75000 + 1 * 65000, calculated from amino acid sequence | Narcissus tazetta |
1.4.3.4 | 75000 | - |
1 * 75000 + 1 * 65000, calculated from amino acid sequence | Narcissus tazetta |
1.4.3.4 | 135000 | - |
gel filtration | Narcissus tazetta |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.3.4 | Narcissus tazetta | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.3.4 | ammonium sulfate precipitation, Sephadex G-25 gel filtration, DEAE Sephacel gel filtration, and hydroxyapatite column chromatography | Narcissus tazetta |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.4.3.4 | shoot | - |
Narcissus tazetta | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.4.3.4 | 0.0032 | - |
crude extract, at pH 7.0 and 37°C | Narcissus tazetta |
1.4.3.4 | 1.365 | - |
1544fold purified enzyme, at pH 7.0 and 37°C | Narcissus tazetta |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.3.4 | 2-phenylethylamine + H2O + O2 | 9% activity compared to n-propylamine | Narcissus tazetta | 2-phenylethanal + NH3 + H2O2 | - |
? | |
1.4.3.4 | benzylamine + H2O + O2 | 74% activity compared to n-propylamine | Narcissus tazetta | benzaldehyde + NH3 + H2O2 | - |
? | |
1.4.3.4 | ethanolamine + H2O + O2 | 50% activity compared to n-propylamine | Narcissus tazetta | glycolaldehyde + NH3 + H2O2 | - |
? | |
1.4.3.4 | ethylamine + H2O + O2 | 84% activity compared to n-propylamine | Narcissus tazetta | ethanal + NH3 + H2O2 | - |
? | |
1.4.3.4 | hexylamine + H2O + O2 | 7% activity compared to n-propylamine | Narcissus tazetta | hexanal + NH3 + H2O2 | - |
? | |
1.4.3.4 | methylamine + H2O + O2 | 34% activity compared to n-propylamine | Narcissus tazetta | formaldehyde + NH3 + H2O2 | - |
? | |
1.4.3.4 | additional information | no activity with tryptamine, serotonin, dopamine, tyramine, histamine, putrescine, cadaverine, diaminodecane, spermidine, and spermine | Narcissus tazetta | ? | - |
? | |
1.4.3.4 | n-butylamine + H2O + O2 | 98% activity compared to n-propylamine | Narcissus tazetta | butyraldehyde + NH3 + H2O2 | - |
? | |
1.4.3.4 | n-pentylamine + H2O + O2 | 56% activity compared to n-propylamine | Narcissus tazetta | pentaldehyde + NH3 + H2O2 | - |
? | |
1.4.3.4 | n-propylamine + H2O + O2 | 100% activity | Narcissus tazetta | propionaldehyde + NH3 + H2O2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.4.3.4 | heterodimer | 1 * 75000 + 1 * 65000, calculated from amino acid sequence | Narcissus tazetta |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.3.4 | MAO | - |
Narcissus tazetta |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.3.4 | 50 | 80 | the purified enzyme retains its activity at temperatures up to 70°C but loses activity quickly after pre-incubation at temperatures of 75°C (less than 50%) and higher | Narcissus tazetta |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.3.4 | 7 | - |
- |
Narcissus tazetta |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.4.3.4 | 6 | 8 | - |
Narcissus tazetta |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.3.4 | FAD | - |
Narcissus tazetta |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
1.4.3.4 | 1 | - |
at pH 7.0 and 37°C | Narcissus tazetta | 2,2'-bipyridyl |