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Literature summary extracted from

  • Enya, M.; Aoyagi, K.; Hishikawa, Y.; Yoshimura, A.; Mitsukura, K.; Maruyama, K.
    Molecular and catalytic properties of 2,4'-dihydroxyacetophenone dioxygenase from Burkholderia sp. AZ11 (2012), Biosci. Biotechnol. Biochem., 76, 567-574.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.41 2-mercaptoethanol slight activation Burkholderia sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.41 gene dad, DNA and amino acid sequence determination and analysis, sequence comparisons, overexpression in Escherichia coli strain JM109 Burkholderia sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.41 1,10-phenanthroline
-
Burkholderia sp.
1.13.11.41 2,2'-dipyridine
-
Burkholderia sp.
1.13.11.41 4-chloromercuribenzoate
-
Burkholderia sp.
1.13.11.41 8-hydroxyquinoline
-
Burkholderia sp.
1.13.11.41 Co2+
-
Burkholderia sp.
1.13.11.41 Cu2+ strong inhibition Burkholderia sp.
1.13.11.41 diethyldicarbonate
-
Burkholderia sp.
1.13.11.41 Hg2+ strong inhibition Burkholderia sp.
1.13.11.41 Ni2+
-
Burkholderia sp.
1.13.11.41 Tiron
-
Burkholderia sp.
1.13.11.41 Zn2+
-
Burkholderia sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.41 0.0016
-
2,4'-dihydroxyacetophenone pH 7.0, 25°C, recombinant enzyme Burkholderia sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.41 Fe2+ the enzyme contains 1.69 mol of non-heme iron per mol of enzyme, His77, His79, His115, and Glu96 in the cupin fold are putative metal ligands, Fe2+ weakly activates the enzyme Burkholderia sp.
1.13.11.41 additional information poor or no effects by Fe3+, Ca2+ and Mg2+ Burkholderia sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.41 23000
-
4 * 23000, recombinant enzyme, SDS-PAGE Burkholderia sp.
1.13.11.41 90000
-
recombinant enzyme, gel filtration Burkholderia sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.41 2,4'-dihydroxyacetophenone + O2 Burkholderia sp.
-
4-hydroxybenzoate + formate
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.41 Burkholderia sp. F7J5X4 isolated from a soil ssampple in Gifu, Japan, gene dad
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.41 recombinant enzyme 39fold from Escherichia coli strain JM109 by heat treatment, ammonium sulfate fractionation, gel filtration, and anion exchange chromatography, native enzyme 17fold from strain AZ11 by gel filtration, anion exchange and hydrophobic interaction chromatography Burkholderia sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.41 0.042
-
crude enzyme extract of strain AZ11, pH 7.0, 25°C Burkholderia sp.
1.13.11.41 0.33
-
crude enzyme extract of recombinant Escherichia coli strain JM109 expressing gene dad, pH 7.0, 25°C Burkholderia sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.41 2,4'-dihydroxyacetophenone + O2
-
Burkholderia sp. 4-hydroxybenzoate + formate
-
?
1.13.11.41 2,4'-dihydroxyacetophenone + O2 anaerobic conditions Burkholderia sp. 4-hydroxybenzoate + formate
-
?
1.13.11.41 additional information 2-hydroxyacetophenone is a poor substrate Burkholderia sp. ?
-
?

Subunits

EC Number Subunits Comment Organism
1.13.11.41 homotetramer 4 * 23000, recombinant enzyme, SDS-PAGE Burkholderia sp.

Synonyms

EC Number Synonyms Comment Organism
1.13.11.41 DAD
-
Burkholderia sp.
1.13.11.41 DHAP dioxygenase
-
Burkholderia sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.41 25
-
assay at Burkholderia sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.41 70
-
purified enzyme, 20 min, over 80% activity remaining Burkholderia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.41 6 7
-
Burkholderia sp.

General Information

EC Number General Information Comment Organism
1.13.11.41 metabolism the enzyme is involved in degradation of bisphenol A Burkholderia sp.
1.13.11.41 physiological function the enzyme is involved in degradation of bisphenol A Burkholderia sp.