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Literature summary extracted from

  • Kim, S.; Lee, S.B.
    Thermostable esterase from a thermoacidophilic archaeon: purification and characterization for enzymatic resolution of a chiral compound (2004), Biosci. Biotechnol. Biochem., 68, 2289-2298.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.1 ethanol 5% (w/v), 1.1fold activation Saccharolobus solfataricus
3.1.1.1 Tween 60 5% (w/v), 1.6fold activation Saccharolobus solfataricus

Application

EC Number Application Comment Organism
3.1.1.1 synthesis synthesis of chiral compounds Saccharolobus solfataricus
3.1.1.1 synthesis the enzyme can be used for application in the synthesis of chiral compounds Saccharolobus solfataricus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.1 expressed in Escherichia coli Saccharolobus solfataricus
3.1.1.1 expression in Escherichia coli as a 6His-tagged fusion protein Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 1-butanol 5% (v/v), 95% inhibition; 5% (w/v), 95% inhibition Saccharolobus solfataricus
3.1.1.1 1-propanol 5% (v/v), 91% inhibition; 5% (w/v), 91% inhibition Saccharolobus solfataricus
3.1.1.1 2-butanol 10% (v/v), 52% inhibition; 10% (w/v), 42% inhibition Saccharolobus solfataricus
3.1.1.1 2-mercaptoethanol 1 mM, 8% inhibition Saccharolobus solfataricus
3.1.1.1 2-propanol 10% (v/v), 94% inhibition; 5% (w/v), 58% inhibition. 10% (w/v), 94% inhibition Saccharolobus solfataricus
3.1.1.1 acetone 10% (v/v), 88% inhibition; 10% (w/v), 88% inhibition Saccharolobus solfataricus
3.1.1.1 acetonitrile 10% (w/v), 61% inhibition; 5% (v/v), 61% inhibition Saccharolobus solfataricus
3.1.1.1 diethyl 4-nitrophenyl phosphate 1 mM, 70% inhibition Saccharolobus solfataricus
3.1.1.1 diethyl dicarbonate 1 mM, 19% inhibition Saccharolobus solfataricus
3.1.1.1 Diethyl p-nitrophenyl phosphate 1 mM, 70% inhibition Saccharolobus solfataricus
3.1.1.1 DMSO 10% (w/v), 66% inhibition; 5% (v/v), 58% inhibition Saccharolobus solfataricus
3.1.1.1 eserine 1 mM, 12% inhibition Saccharolobus solfataricus
3.1.1.1 HgCl2 1 mM, 53% inhibition Saccharolobus solfataricus
3.1.1.1 methanol 10% (v/v), 93% inhibition; 5% (w/v), 94% inhibition Saccharolobus solfataricus
3.1.1.1 additional information no inhibition by EDTA Saccharolobus solfataricus
3.1.1.1 phenylmethylsulfonyl fluoride 1 mM, 72% inhibition Saccharolobus solfataricus
3.1.1.1 Triton X-100 10% (v/v), 92% inhibition; 10% (w/v), 92% inhibition Saccharolobus solfataricus
3.1.1.1 Tween 5% (v/v), 94% inhibition Saccharolobus solfataricus
3.1.1.1 Tween 20 5% (w/v), 94% inhibition Saccharolobus solfataricus
3.1.1.1 Tween 50 10% (v/v), 49% inhibition Saccharolobus solfataricus
3.1.1.1 Tween 60 10% (w/v), 49% inhibition Saccharolobus solfataricus
3.1.1.1 Tween 80 5% (w/v), 8% inhibition Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 2.1
-
 4-nitrophenyl pentanoate pH 7.0, 70°C Saccharolobus solfataricus
3.1.1.1 2.1
-
 4-nitrophenyl pentanoate 70°C, pH 7.2 Saccharolobus solfataricus
3.1.1.1 2.5
-
 4-nitrophenyl acetate pH 7.0, 70°C Saccharolobus solfataricus
3.1.1.1 2.5
-
 4-nitrophenyl acetate 70°C, pH 7.2 Saccharolobus solfataricus
3.1.1.1 4.8
-
 4-nitrophenyl hexadecanoate pH 7.0, 70°C Saccharolobus solfataricus
3.1.1.1 4.8
-
 4-nitrophenyl hexadecanoate 70°C, pH 7.2 Saccharolobus solfataricus
3.1.1.1 14.5
-
 4-nitrophenyl octanoate pH 7.0, 70°C Saccharolobus solfataricus
3.1.1.1 14.5
-
 4-nitrophenyl hexanoate 70°C, pH 7.2 Saccharolobus solfataricus
3.1.1.1 67.5
-
 4-nitrophenyl dodecanoate pH 7.0, 70°C Saccharolobus solfataricus
3.1.1.1 67.6
-
 4-nitrophenyl dodecanoate 70°C, pH 7.2 Saccharolobus solfataricus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.1 intracellular
-
 Saccharolobus solfataricus 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 32000
-
 x * 32000 Saccharolobus solfataricus
3.1.1.1 32000
-
 3 * 32000 Saccharolobus solfataricus
3.1.1.1 33428
-
 3 * 33428, calculated from sequence Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Saccharolobus solfataricus Q97VW1
-
-
3.1.1.1 Saccharolobus solfataricus P2 Q97VW1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1
-
 Saccharolobus solfataricus
3.1.1.1 recombinant enzyme Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.1 3133
-
 70°C, pH 7.2 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 (R)-2-(3-benzoylphenyl)propanoic methyl ester + H2O when tested with the racemic substrate, the enzyme leads to the production of the R-configured acid with an enantiomeric excess of 80% at the conversion rate of 20% in 32 h Saccharolobus solfataricus methanol + (R)-2-(3-benzoylphenyl)propanoate
-
 ?
3.1.1.1 4-nitrophenyl acetate + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl acetate + H2O hydrolysis at about 70% compared to activity with 4-nitrophenyl pentanoate Saccharolobus solfataricus 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl acetate + H2O
-
 Saccharolobus solfataricus P2 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl acetate + H2O hydrolysis at about 70% compared to activity with 4-nitrophenyl pentanoate Saccharolobus solfataricus P2 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl butanoate + H2O hydrolysis at about 90% compared to activity with 4-nitrophenyl pentanoate Saccharolobus solfataricus 4-nitrophenol + butanoate
-
 ?
3.1.1.1 4-nitrophenyl decanoate + H2O hydrolysis at about 50% compared to activity with 4-nitrophenyl pentanoate Saccharolobus solfataricus 4-nitrophenol + decanoate
-
 ?
3.1.1.1 4-nitrophenyl dodecanoate + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + dodecanoate
-
 ?
3.1.1.1 4-nitrophenyl dodecanoate + H2O hydrolysis at about 30% compared to activity with 4-nitrophenyl pentanoate Saccharolobus solfataricus 4-nitrophenol + dodecanoate
-
 ?
3.1.1.1 4-nitrophenyl hexadecanoate + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + hexadecanoate
-
 ?
3.1.1.1 4-nitrophenyl hexadecanoate + H2O hydrolysis at less than 10% compared to activity with 4-nitrophenyl pentanoate Saccharolobus solfataricus 4-nitrophenol + hexadecanoate
-
 ?
3.1.1.1 4-nitrophenyl hexanoate + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + hexanoate
-
 ?
3.1.1.1 4-nitrophenyl hexanoate + H2O hydrolysis at about 90% compared to activity with 4-nitrophenyl pentanoate Saccharolobus solfataricus 4-nitrophenol + hexanoate
-
 ?
3.1.1.1 4-nitrophenyl hexanoate + H2O
-
 Saccharolobus solfataricus P2 4-nitrophenol + hexanoate
-
 ?
3.1.1.1 4-nitrophenyl octanoate + H2O hydrolysis at about 75% compared to activity with 4-nitrophenyl pentanoate Saccharolobus solfataricus 4-nitrophenol + octanoate
-
 ?
3.1.1.1 4-nitrophenyl pentanoate + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + pentanoate
-
 ?
3.1.1.1 4-nitrophenyl pentanoate + H2O
-
 Saccharolobus solfataricus P2 4-nitrophenol + pentanoate
-
 ?
3.1.1.1 4-nitrophenyl propanoate + H2O hydrolysis at about 85% compared to activity with 4-nitrophenyl pentanoate Saccharolobus solfataricus 4-nitrophenol + propanoate
-
 ?
3.1.1.1 4-nitrophenyl tetradecanoate + H2O hydrolysis at less tha 10% compared to activity with 4-nitrophenyl pentanoate Saccharolobus solfataricus 4-nitrophenol + tetradecanoate
-
 ?
3.1.1.1 methyl (-)-2-(3-benzoylphenyl)propionate + H2O
-
 Saccharolobus solfataricus (-)-2-(3-benzoylphenyl)propionate + methanol
-
 ?
3.1.1.1 additional information broad substrate specificities toward various p-nitrophenyl esters ranging from C2 to C16. The activity of the enzyme decreases slowly from 4-nitrophenyl hexanoate to 4-nitrophenyl dodecanoate and is very low for 4-nitrophenyl 4-nitrophenyl hexadecanoate Saccharolobus solfataricus ?
-
 ?
3.1.1.1 additional information broad substrate specificities toward various p-nitrophenyl esters ranging from C2 to C16. The activity of the enzyme decreases slowly from 4-nitrophenyl hexanoate to 4-nitrophenyl dodecanoate and is very low for 4-nitrophenyl 4-nitrophenyl hexadecanoate Saccharolobus solfataricus P2 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 trimer x * 32000 Saccharolobus solfataricus
3.1.1.1 trimer 3 * 32000 Saccharolobus solfataricus
3.1.1.1 trimer 3 * 33428, calculated from sequence Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 ESt3
-
 Saccharolobus solfataricus
3.1.1.1 Est3 esterase
-
 Saccharolobus solfataricus
3.1.1.1 SSO2493 locus name Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 70
-
 assay at Saccharolobus solfataricus
3.1.1.1 80
-
-
 Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.1 40 90 40°C: about 50% of maximal activity, 90°C: about 70% of maximal activity Saccharolobus solfataricus
3.1.1.1 60 90 60°C: about 60% of maximal activity, 90°C: about 70% of maximal activity Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 60
-
 2 h, less than 10% loss of activity Saccharolobus solfataricus
3.1.1.1 70
-
 2 h, less than 10% loss of activity Saccharolobus solfataricus
3.1.1.1 80
-
 half-life: 40 min Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.1 3.8
-
 4-nitrophenyl hexadecanoate 70°C, pH 7.2 Saccharolobus solfataricus
3.1.1.1 3.8
-
 4-nitrophenyl hexadecanoate pH 7.0, 70°C Saccharolobus solfataricus
3.1.1.1 45.3
-
 4-nitrophenyl acetate 70°C, pH 7.2 Saccharolobus solfataricus
3.1.1.1 45.3
-
 4-nitrophenyl acetate pH 7.0, 70°C Saccharolobus solfataricus
3.1.1.1 46.3
-
 4-nitrophenyl pentanoate 70°C, pH 7.2 Saccharolobus solfataricus
3.1.1.1 46.3
-
 4-nitrophenyl pentanoate pH 7.0, 70°C Saccharolobus solfataricus
3.1.1.1 78
-
 4-nitrophenyl dodecanoate 70°C, pH 7.2 Saccharolobus solfataricus
3.1.1.1 78
-
 4-nitrophenyl dodecanoate pH 7.0, 70°C Saccharolobus solfataricus
3.1.1.1 79.3
-
 4-nitrophenyl hexanoate 70°C, pH 7.2 Saccharolobus solfataricus
3.1.1.1 79.3
-
 4-nitrophenyl octanoate pH 7.0, 70°C Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 7.2
-
 assay at Saccharolobus solfataricus
3.1.1.1 7.4
-
-
 Saccharolobus solfataricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.1 6.5 10 pH 6.5: about 40% of maximal activity, pH 10.0: about 50% of maximal activity Saccharolobus solfataricus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.1 0.7
-
 4-nitrophenyl hexadecanoate pH 7.0, 70°C Saccharolobus solfataricus
3.1.1.1 0.7
-
 4-nitrophenyl hexadecanoate 70°C, pH 7.2 Saccharolobus solfataricus
3.1.1.1 1.2
-
 4-nitrophenyl dodecanoate pH 7.0, 70°C Saccharolobus solfataricus
3.1.1.1 1.2
-
 4-nitrophenyl dodecanoate 70°C, pH 7.2 Saccharolobus solfataricus
3.1.1.1 5.1
-
 4-nitrophenyl octanoate pH 7.0, 70°C Saccharolobus solfataricus
3.1.1.1 5.1
-
 4-nitrophenyl hexanoate 70°C, pH 7.2 Saccharolobus solfataricus
3.1.1.1 16.2
-
 4-nitrophenyl acetate pH 7.0, 70°C Saccharolobus solfataricus
3.1.1.1 16.2
-
 4-nitrophenyl acetate 70°C, pH 7.2 Saccharolobus solfataricus
3.1.1.1 21.1
-
 4-nitrophenyl pentanoate pH 7.0, 70°C Saccharolobus solfataricus
3.1.1.1 21.1
-
 4-nitrophenyl pentanoate 70°C, pH 7.2 Saccharolobus solfataricus