EC Number | Application | Comment | Organism |
---|---|---|---|
1.14.13.196 | drug development | essential for virulence, validating this enzyme as a drug target | Aspergillus fumigatus |
EC Number | General Stability | Organism |
---|---|---|
1.14.13.196 | NADP+, and not NAD+, protects the enzyme from proteolysis | Aspergillus fumigatus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.195 | 0.012 | - |
NADPH | pH 7.5, 15°C | Aspergillus fumigatus | |
1.14.13.195 | 0.1 | - |
NADH | pH 7.5, 15°C | Aspergillus fumigatus | |
1.14.13.196 | 0.012 | - |
NADPH | pH 7.5, temperature not specified in the publication | Aspergillus fumigatus | |
1.14.13.196 | 0.1 | - |
NADH | pH 7.5, temperature not specified in the publication | Aspergillus fumigatus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.196 | L-ornithine + NADH + H+ + O2 | Aspergillus fumigatus | first step in the biosynthesis of hydroxamate-containing siderophores, essential for virulence | N5-hydroxy-L-ornithine + NAD+ + H2O | - |
? | |
1.14.13.196 | L-ornithine + NADPH + H+ + O2 | Aspergillus fumigatus | first step in the biosynthesis of hydroxamate-containing siderophores, essential for virulence | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.195 | Aspergillus fumigatus | Q5SE95 | - |
- |
1.14.13.196 | Aspergillus fumigatus | Q5SE95 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.196 | - |
Aspergillus fumigatus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.195 | L-ornithine + NADH + H+ + O2 | - |
Aspergillus fumigatus | N5-hydroxy-L-ornithine + NAD+ + H2O | - |
? | |
1.14.13.195 | L-ornithine + NADPH + H+ + O2 | - |
Aspergillus fumigatus | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
1.14.13.195 | additional information | when the enzyme is reduced with NADPH and reacts with molecular oxygen, a C4a-hydroperoxyflavin intermediate is observed. When the enzyme is reduced with NADH, the intermediate is 2fold less stable. Steady-state kinetic isotope effect values for NADPH and NADH are 3 and 2 , respectively, due to differences in the rate of flavin reduction by these coenzymes. NADP+, and not NAD+, protects the enzyme from proteolysis, suggesting that it induces conformational changes upon binding | Aspergillus fumigatus | ? | - |
? | |
1.14.13.196 | L-ornithine + NADH + H+ + O2 | first step in the biosynthesis of hydroxamate-containing siderophores, essential for virulence | Aspergillus fumigatus | N5-hydroxy-L-ornithine + NAD+ + H2O | - |
? | |
1.14.13.196 | L-ornithine + NADH + H+ + O2 | accepts reducing equivalents from either NADPH or NADH and displays similar kinetic parameters when using either coenzyme | Aspergillus fumigatus | N5-hydroxy-L-ornithine + NAD+ + H2O | - |
? | |
1.14.13.196 | L-ornithine + NADPH + H+ + O2 | first step in the biosynthesis of hydroxamate-containing siderophores, essential for virulence | Aspergillus fumigatus | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
1.14.13.196 | L-ornithine + NADPH + H+ + O2 | accepts reducing equivalents from either NADPH or NADH and displays similar kinetic parameters when using either coenzyme | Aspergillus fumigatus | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.195 | SidA | - |
Aspergillus fumigatus |
1.14.13.195 | siderophore A | - |
Aspergillus fumigatus |
1.14.13.196 | SidA | - |
Aspergillus fumigatus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.195 | 0.6 | - |
NADPH | pH 7.5, 15°C | Aspergillus fumigatus | |
1.14.13.195 | 0.73 | - |
NADH | pH 7.5, 15°C | Aspergillus fumigatus | |
1.14.13.196 | 0.6 | - |
NADPH | pH 7.5, temperature not specified in the publication | Aspergillus fumigatus | |
1.14.13.196 | 0.73 | - |
NADH | pH 7.5, temperature not specified in the publication | Aspergillus fumigatus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.196 | NADH | accepts reducing equivalents from either NADPH or NADH and displays similar kinetic parameters when using either coenzyme | Aspergillus fumigatus | |
1.14.13.196 | NADPH | accepts reducing equivalents from either NADPH or NADH and displays similar kinetic parameters when using either coenzyme. NADP+, and not NAD+, protects the enzyme from proteolysis | Aspergillus fumigatus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.13.195 | physiological function | enzyme is essential for virulence | Aspergillus fumigatus |
1.14.13.196 | physiological function | first step in the biosynthesis of hydroxamate-containing siderophores, essential for virulence | Aspergillus fumigatus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.195 | 10 | - |
NADH | pH 7.5, 15°C | Aspergillus fumigatus | |
1.14.13.195 | 50 | - |
NADPH | pH 7.5, 15°C | Aspergillus fumigatus | |
1.14.13.196 | 7.3 | - |
NADH | pH 7.5, temperature not specified in the publication | Aspergillus fumigatus | |
1.14.13.196 | 50 | - |
NADPH | pH 7.5, temperature not specified in the publication | Aspergillus fumigatus |