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Literature summary extracted from

  • Langston, J.A.; Brown, K.; Xu, F.; Borch, K.; Garner, A.; Sweeney, M.D.
    Cloning, expression, and characterization of a cellobiose dehydrogenase from Thielavia terrestris induced under cellulose growth conditions (2012), Biochim. Biophys. Acta, 1824, 802-812.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.18 expressed in Aspergillus oryzae Jal355 cells Thermothielavioides terrestris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.18 0.0039
-
 cellobiose in 50 mM MES buffer (pH 6.0), at 25°C Thermothielavioides terrestris
1.1.99.18 0.0056
-
 cellotetraose in 50 mM MES buffer (pH 6.0), at 25°C Thermothielavioides terrestris
1.1.99.18 0.0075
-
 cellopentaose in 50 mM MES buffer (pH 6.0), at 25°C Thermothielavioides terrestris
1.1.99.18 0.0082
-
 cellotriose in 50 mM MES buffer (pH 6.0), at 25°C Thermothielavioides terrestris
1.1.99.18 0.023
-
 lactose in 50 mM MES buffer (pH 6.0), at 25°C Thermothielavioides terrestris
1.1.99.18 0.078
-
 2,6-dichlorophenol-indophenol in 50 mM MES buffer (pH 6.0), at 25°C Thermothielavioides terrestris
1.1.99.18 2.5
-
 maltose in 50 mM MES buffer (pH 6.0), at 25°C Thermothielavioides terrestris
1.1.99.18 54
-
 D-glucose in 50 mM MES buffer (pH 6.0), at 25°C Thermothielavioides terrestris
1.1.99.18 1000
-
 D-xylose in 50 mM MES buffer (pH 6.0), at 25°C Thermothielavioides terrestris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.18 additional information secretome Thermothielavioides terrestris
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.18 125000
-
 x * 125000, SDS-PAGE Thermothielavioides terrestris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.99.18 cellobiose + 2,6-dichlorophenol-indophenol Thermothielavioides terrestris
-
 cellobiono-1,5-lactone + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.1.99.18 cellobiose + 2,6-dichlorophenol-indophenol Thermothielavioides terrestris NRRL8126
-
 cellobiono-1,5-lactone + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.1.99.18 cellopentaose + 2,6-dichlorophenol-indophenol Thermothielavioides terrestris
-
 ? + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.1.99.18 cellopentaose + 2,6-dichlorophenol-indophenol Thermothielavioides terrestris NRRL8126
-
 ? + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.1.99.18 cellotetraose + 2,6-dichlorophenol-indophenol Thermothielavioides terrestris
-
 ? + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.1.99.18 cellotetraose + 2,6-dichlorophenol-indophenol Thermothielavioides terrestris NRRL8126
-
 ? + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.1.99.18 cellotriose + 2,6-dichlorophenol-indophenol Thermothielavioides terrestris
-
 ? + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.1.99.18 cellotriose + 2,6-dichlorophenol-indophenol Thermothielavioides terrestris NRRL8126
-
 ? + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.1.99.18 D-glucose + 2,6-dichlorophenol-indophenol Thermothielavioides terrestris
-
 ? + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.1.99.18 D-xylose + 2,6-dichlorophenol-indophenol Thermothielavioides terrestris
-
 ? + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.1.99.18 lactose + 2,6-dichlorophenol-indophenol Thermothielavioides terrestris
-
 ? + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.1.99.18 maltose + 2,6-dichlorophenol-indophenol Thermothielavioides terrestris
-
 ? + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.1.99.18 additional information Thermothielavioides terrestris when incubated with insoluble microcrystalline cellulose (avicel) at pH 5.0 and ambient temperature (about 24°C), only 0.1% of the original enzyme activity remains in the supernatant as assayed with cellobiose and 2,6-dichlorophenol-indophenol ?
-
 ?
1.1.99.18 additional information Thermothielavioides terrestris NRRL8126 when incubated with insoluble microcrystalline cellulose (avicel) at pH 5.0 and ambient temperature (about 24°C), only 0.1% of the original enzyme activity remains in the supernatant as assayed with cellobiose and 2,6-dichlorophenol-indophenol ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.18 Thermothielavioides terrestris
-
-
-
1.1.99.18 Thermothielavioides terrestris NRRL8126
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.99.18 glycoprotein approximately 40% of the expressed and processed protein represents glycosylation Thermothielavioides terrestris

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.18 Mono Q column chromatography and Source 15PHE column chromatography Thermothielavioides terrestris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.18 0.85
-
 purified enzyme, with cellobiose as substrate, in 50 mM MES buffer (pH 6.0), at 25°C Thermothielavioides terrestris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.18 cellobiose + 2,6-dichlorophenol-indophenol
-
 Thermothielavioides terrestris cellobiono-1,5-lactone + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.1.99.18 cellobiose + 2,6-dichlorophenol-indophenol
-
 Thermothielavioides terrestris NRRL8126 cellobiono-1,5-lactone + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.1.99.18 cellopentaose + 2,6-dichlorophenol-indophenol
-
 Thermothielavioides terrestris ? + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.1.99.18 cellopentaose + 2,6-dichlorophenol-indophenol
-
 Thermothielavioides terrestris NRRL8126 ? + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.1.99.18 cellotetraose + 2,6-dichlorophenol-indophenol
-
 Thermothielavioides terrestris ? + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.1.99.18 cellotetraose + 2,6-dichlorophenol-indophenol
-
 Thermothielavioides terrestris NRRL8126 ? + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.1.99.18 cellotriose + 2,6-dichlorophenol-indophenol
-
 Thermothielavioides terrestris ? + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.1.99.18 cellotriose + 2,6-dichlorophenol-indophenol
-
 Thermothielavioides terrestris NRRL8126 ? + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.1.99.18 D-glucose + 2,6-dichlorophenol-indophenol
-
 Thermothielavioides terrestris ? + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.1.99.18 D-xylose + 2,6-dichlorophenol-indophenol
-
 Thermothielavioides terrestris ? + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.1.99.18 lactose + 2,6-dichlorophenol-indophenol
-
 Thermothielavioides terrestris ? + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.1.99.18 maltose + 2,6-dichlorophenol-indophenol
-
 Thermothielavioides terrestris ? + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.1.99.18 additional information when incubated with insoluble microcrystalline cellulose (avicel) at pH 5.0 and ambient temperature (about 24°C), only 0.1% of the original enzyme activity remains in the supernatant as assayed with cellobiose and 2,6-dichlorophenol-indophenol Thermothielavioides terrestris ?
-
 ?
1.1.99.18 additional information when incubated with insoluble microcrystalline cellulose (avicel) at pH 5.0 and ambient temperature (about 24°C), only 0.1% of the original enzyme activity remains in the supernatant as assayed with cellobiose and 2,6-dichlorophenol-indophenol Thermothielavioides terrestris NRRL8126 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.18 ? x * 125000, SDS-PAGE Thermothielavioides terrestris

Synonyms

EC Number Synonyms Comment Organism
1.1.99.18 cbdA
-
 Thermothielavioides terrestris
1.1.99.18 Cdh
-
 Thermothielavioides terrestris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.99.18 65 87 the enzyme unfolds over the range of 65-87°C, with a Tm value of 80.2°C Thermothielavioides terrestris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.18 0.17
-
 maltose in 50 mM MES buffer (pH 6.0), at 25°C Thermothielavioides terrestris
1.1.99.18 0.33
-
 D-xylose in 50 mM MES buffer (pH 6.0), at 25°C Thermothielavioides terrestris
1.1.99.18 1.01
-
 D-glucose in 50 mM MES buffer (pH 6.0), at 25°C Thermothielavioides terrestris
1.1.99.18 1.23
-
 cellobiose in 50 mM MES buffer (pH 6.0), at 25°C Thermothielavioides terrestris
1.1.99.18 1.23
-
 cellotetraose in 50 mM MES buffer (pH 6.0), at 25°C Thermothielavioides terrestris
1.1.99.18 1.23
-
 cellopentaose in 50 mM MES buffer (pH 6.0), at 25°C Thermothielavioides terrestris
1.1.99.18 1.25
-
 cellotriose in 50 mM MES buffer (pH 6.0), at 25°C Thermothielavioides terrestris
1.1.99.18 1.4
-
 lactose in 50 mM MES buffer (pH 6.0), at 25°C Thermothielavioides terrestris
1.1.99.18 2.05
-
 2,6-dichlorophenol-indophenol in 50 mM MES buffer (pH 6.0), at 25°C Thermothielavioides terrestris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.18 6
-
-
 Thermothielavioides terrestris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.99.18 4 6 below pH 4.0, and particularly above pH 6.0, the activity is observed to drop (65% activity at pH 3.0, 45% activity at pH 7.0 and no activity at pH 9.0) Thermothielavioides terrestris

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.18 FAD
-
 Thermothielavioides terrestris
1.1.99.18 heme
-
 Thermothielavioides terrestris

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.99.18 0.00033
-
 D-xylose in 50 mM MES buffer (pH 6.0), at 25°C Thermothielavioides terrestris
1.1.99.18 0.019
-
 D-glucose in 50 mM MES buffer (pH 6.0), at 25°C Thermothielavioides terrestris
1.1.99.18 0.068
-
 maltose in 50 mM MES buffer (pH 6.0), at 25°C Thermothielavioides terrestris
1.1.99.18 62
-
 lactose in 50 mM MES buffer (pH 6.0), at 25°C Thermothielavioides terrestris
1.1.99.18 150
-
 cellotriose in 50 mM MES buffer (pH 6.0), at 25°C Thermothielavioides terrestris
1.1.99.18 160
-
 cellopentaose in 50 mM MES buffer (pH 6.0), at 25°C Thermothielavioides terrestris
1.1.99.18 220
-
 cellotetraose in 50 mM MES buffer (pH 6.0), at 25°C Thermothielavioides terrestris
1.1.99.18 320
-
 cellobiose in 50 mM MES buffer (pH 6.0), at 25°C Thermothielavioides terrestris