Literature summary extracted from

Fugate, C.J.; Jarrett, J.T.
Biotin synthase: insights into radical-mediated carbon-sulfur bond formation (2012), Biochim. Biophys. Acta, 1824, 1213-1222.

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.8.1.6	0.005	-	dethiobiotin	pH and temperature not specified in the publication	Escherichia coli
2.8.1.6	0.2	-	9-Mercaptodethiobiotin	pH and temperature not specified in the publication	Escherichia coli

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.8.1.6	38600	-	2 * 38600, calculated from amino acid sequence	Escherichia coli

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.8.1.6	Escherichia coli	P12996	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.8.1.6	9-mercaptodethiobiotin + [S] + S-adenosyl-L-methionine	-	Escherichia coli	biotin + ?	-	?
2.8.1.6	dethiobiotin + [S] + S-adenosyl-L-methionine	dethiobiotin is 5-methyl-2-oxo-4-imidazolidinehexanoic acid	Escherichia coli	biotin + L-methionine + 5'-deoxyadenosine	-	?

Subunits

EC Number	Subunits	Comment	Organism
2.8.1.6	homodimer	2 * 38600, calculated from amino acid sequence	Escherichia coli

Synonyms

EC Number	Synonyms	Comment	Organism
2.8.1.6	BioB	-	Escherichia coli

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
2.8.1.6	Fe-S center	the enzyme contains one [4Fe-4S]2+ cluster and one air-stable [2Fe-2S]2+ cluster per monomer	Escherichia coli

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Release 2023.1 (January 2023)