Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hofbauer, S.; Gysel, K.; Mlynek, G.; Kostan, J.; Hagmueller, A.; Daims, H.; Furtmueller, P.; Djinovic-Carugo, K.; Obinger, C.
    Impact of subunit and oligomeric structure on the thermal and conformational stability of chlorite dismutases (2012), Biochim. Biophys. Acta, 1824, 1031-1038.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.13.11.49 environmental protection the enzyme from Nitrospira defluvii is an interesting candidate for bioremediation of chlorite Nitrospira defluvii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.49 expression of C-terminally His-tagged enzyme in Escherichia coli strain Tuner (DE3) Nitrobacter winogradskyi
1.13.11.49 recombinant expression of the enzyme without the N-terminal signal, but with N-terminal TEV-cleavable Strep-II tag in Escherichia coli strain Tuner (DE3) Nitrospira defluvii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.49 additional information
-
 additional information steady-state kinetics, overview Nitrospira defluvii
1.13.11.49 additional information
-
 additional information steady-state kinetics, overview Nitrobacter winogradskyi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.49 chlorite Nitrospira defluvii
-
 chloride + O2
-
 ?
1.13.11.49 chlorite Nitrobacter winogradskyi
-
 chloride + O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.49 Nitrobacter winogradskyi Q3SPU6
-
-
1.13.11.49 Nitrospira defluvii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.49 recombinant His-tagged enzyme from Escherichia coli strain Tuner (DE3) by affinity chromatography Nitrobacter winogradskyi
1.13.11.49 recombinant Strep-tagged enzyme from Escherichia coli strain Tuner (DE3) by affinity chromatography, the Strep-II-tag is fully cleaved off using TEV-protease, followed by gel filtration Nitrospira defluvii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.49 chlorite
-
 Nitrospira defluvii chloride + O2
-
 ?
1.13.11.49 chlorite
-
 Nitrobacter winogradskyi chloride + O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.49 dimer conformational and thermal stability of recombinant dimeric Cld from Nitrobacter winogradskyi, overview Nitrobacter winogradskyi
1.13.11.49 pentamer conformational and thermal stability of recombinant pentameric Cld from Nitrospira defluvii, overview Nitrospira defluvii

Synonyms

EC Number Synonyms Comment Organism
1.13.11.49 chlorite dismutase
-
 Nitrospira defluvii
1.13.11.49 chlorite dismutase
-
 Nitrobacter winogradskyi
1.13.11.49 CLD
-
 Nitrospira defluvii
1.13.11.49 CLD
-
 Nitrobacter winogradskyi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.49 25
-
 assay at Nitrospira defluvii
1.13.11.49 25
-
 assay at Nitrobacter winogradskyi

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.11.49 30 55 activity range Nitrobacter winogradskyi
1.13.11.49 30 90 activity range Nitrospira defluvii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.49 additional information
-
 conformational and thermal stability of recombinant dimeric Cld from Nitrobacter winogradskyi, overview Nitrobacter winogradskyi
1.13.11.49 additional information
-
 conformational and thermal stability of recombinant pentameric Cld from Nitrospira defluvii, overview Nitrospira defluvii
1.13.11.49 40
-
 purified recombinant enzyme, stable up to Nitrospira defluvii
1.13.11.49 40
-
 purified recombinant enzyme, stable up to Nitrobacter winogradskyi
1.13.11.49 50
-
 purified recombinant enzyme, 80% activity remaining Nitrospira defluvii
1.13.11.49 50
-
 purified recombinant enzyme, 8% activity remaining Nitrobacter winogradskyi
1.13.11.49 55
-
 purified recombinant enzyme, inactivation Nitrobacter winogradskyi
1.13.11.49 70
-
 purified recombinant enzyme, 36% activity remaining Nitrospira defluvii
1.13.11.49 90
-
 purified recombinant enzyme, inactivation Nitrospira defluvii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.49 5.5
-
-
 Nitrospira defluvii
1.13.11.49 6
-
-
 Nitrobacter winogradskyi

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.13.11.49 3 10 the pentameric enzyme is very stable between pH 3 and 10 with Tm=92°C at pH 7.0 Nitrospira defluvii
1.13.11.49 7
-
 purified recombinant enzyme, stable Nitrospira defluvii
1.13.11.49 7
-
 purified recombinant enzyme, stable Nitrobacter winogradskyi

General Information

EC Number General Information Comment Organism
1.13.11.49 additional information conformational and thermal stability of recombinant dimeric Cld from Nitrobacter winogradskyi, overview Nitrobacter winogradskyi
1.13.11.49 additional information conformational and thermal stability of recombinant pentameric Cld from Nitrospira defluvii, overview Nitrospira defluvii