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Literature summary extracted from

  • Doan, T.T.; Domergue, F.; Fournier, A.E.; Vishwanath, S.J.; Rowland, O.; Moreau, P.; Wood, C.C.; Carlsson, A.S.; Hamberg, M.; Hofvander, P.
    Biochemical characterization of a chloroplast localized fatty acid reductase from Arabidopsis thaliana (2012), Biochim. Biophys. Acta, 1821, 1244-1255.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.42 expressed in Saccharomyces cerevisiae strain W303-1A and in Nicotiana benthamiana leaves Arabidopsis thaliana
1.2.1.80 AtFAR6 full length version and a truncated version, mAtFAR6, which lacks the chloroplast transit peptide sequence expressed in Escherichia coli, both enzymes fused at the C-terminus to a His Tag containing maltose binding protein Arabidopsis thaliana
1.2.1.80 AtFAR6 full length version and a truncated version, mAtFAR6, which lacks the chloroplast transit peptide sequence subcloned into the pYES-DEST52 plasmid downstream of the GAL1 promoter and transformed into Saccharomyces cerevisiae W303-1A strain Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.42 0.00146
-
 [hexadecanoyl]-acyl carrier protein in 100 mM phosphate buffer, pH 7.0, at 30°C Arabidopsis thaliana
1.2.1.42 0.0036
-
 hexadecanoyl-CoA in 100 mM phosphate buffer, pH 7.0, at 30°C Arabidopsis thaliana
1.2.1.80 0.00148
-
 hexadecanoyl-[acyl-carrier protein] pH 7.0, at 30°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.42 chloroplast
-
 Arabidopsis thaliana 9507
-
1.2.1.80 chloroplast transient expression of fusion proteins of AtFAR6 and yellow fluorescent protein in Nicotiana sp. leaves show that AtFAR6 is chloroplast-localized Arabidopsis thaliana 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.42 heptadecanoyl-CoA + NADPH + H+ Arabidopsis thaliana
-
 heptadecanal + CoA + NADP+
-
 ?
1.2.1.42 hexadecanoyl-CoA + NADPH + H+ Arabidopsis thaliana preferred substrate hexadecanal + CoA + NADP+
-
 ?
1.2.1.42 additional information Arabidopsis thaliana no products are detected when using C18:1-CoA, 2-methyl-16:0-CoA, 2-methyl-18:0-CoA or C16:0 free fatty acid as substrates ?
-
 ?
1.2.1.42 octadecanoyl-CoA + NADPH + H+ Arabidopsis thaliana low activity octadecanal + CoA + NADP+
-
 ?
1.2.1.42 [hexadecanoyl]-acyl carrier protein + NADPH + H+ Arabidopsis thaliana preferred substrate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.42 Arabidopsis thaliana
-
-
-
1.2.1.80 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.42 HisTrap column chromatography and Ni2+ resin column chromatography Arabidopsis thaliana
1.2.1.80 in Escherichia coli expressed AtFAR6 and mAtFAR6 purified by a HisTrap HP column BioLogic LP liquid chromatography system Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.80 flower AtFAR6 promoter active in epidermis, endothecium and tapetum of anthers, histochemical staining of AtFAR6 promoter-GUS reporter gene activity Arabidopsis thaliana
-
1.2.1.80 additional information no AtFAR6 promoter-GUS reporter gene activity in the inner cortex, vascular bundles or flower microspores Arabidopsis thaliana
-
1.2.1.80 plant epidermis AtFAR6 promoter active in stem layer, histochemical staining of AtFAR6 promoter-GUS reporter gene activity Arabidopsis thaliana
-
1.2.1.80 receptacle AtFAR6 promoter active in the replum and receptacle of siliques Arabidopsis thaliana
-
1.2.1.80 root cap throughout lateral and primary root development, histochemical staining of AtFAR6 promoter-GUS reporter gene activity Arabidopsis thaliana
-
1.2.1.80 root primordium histochemical staining of AtFAR6 promoter-GUS reporter gene activity Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.42 heptadecanoyl-CoA + NADPH + H+
-
 Arabidopsis thaliana heptadecanal + CoA + NADP+
-
 ?
1.2.1.42 hexadecanoyl-CoA + NADPH + H+ preferred substrate Arabidopsis thaliana hexadecanal + CoA + NADP+
-
 ?
1.2.1.42 additional information no products are detected when using C18:1-CoA, 2-methyl-16:0-CoA, 2-methyl-18:0-CoA or C16:0 free fatty acid as substrates Arabidopsis thaliana ?
-
 ?
1.2.1.42 octadecanoyl-CoA + NADPH + H+ low activity Arabidopsis thaliana octadecanal + CoA + NADP+
-
 ?
1.2.1.42 [hexadecanoyl]-acyl carrier protein + NADPH + H+ preferred substrate Arabidopsis thaliana ?
-
 ?
1.2.1.80 hexadecanoyl-[acyl-carrier protein] + NAD(P)H + H+ acyl-CoA and acyl-ACP can serve as substrates Arabidopsis thaliana hexadecanal + acyl-carrier protein + NAD(P)+ hexadecanal is a reaction intermediate that is further converted into hexadecanol ?
1.2.1.80 additional information in Saccharomyces cerevisiae overexpressed truncated version of AtFAR6, which lacks the N-terminal extension, produces C16:0 primary fatty alcohol as well as C18:0 fatty alcohols much more efficiently than the full length protein Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.42 FAR6
-
 Arabidopsis thaliana
1.2.1.42 fatty acid reductase
-
 Arabidopsis thaliana
1.2.1.80 AtFAR6
-
 Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.42 4 10
-
 Arabidopsis thaliana
1.2.1.80 4 10 truncated mAtFAR6 protein Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.42 NADPH
-
 Arabidopsis thaliana
1.2.1.80 additional information no significant activity of truncated mAtFAR6 observed when NADH is used as cofactor Arabidopsis thaliana
1.2.1.80 NADPH
-
 Arabidopsis thaliana