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Literature summary extracted from

  • Arias, D.G.; Regner, E.L.; Iglesias, A.A.; Guerrero, S.A.
    Entamoeba histolytica thioredoxin reductase: molecular and functional characterization of its atypical properties (2012), Biochim. Biophys. Acta, 1820, 1859-1866.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.9 expressed in Escherichia coli BL21(DE3) cells Entamoeba histolytica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.9 Cu2+
-
 Entamoeba histolytica
1.8.1.9 NAD+ NAD+ acts as poor competitive inhibitor respect to both NADPH and NADH Entamoeba histolytica
1.8.1.9 NADP+ better competitive inhibitor than NAD+ Entamoeba histolytica
1.8.1.9 Zn2+
-
 Entamoeba histolytica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.9 0.0018
-
 NADPH with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.8.1.9 0.0023
-
 thioredoxin 41 with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.8.1.9 0.0028
-
 thioredoxin 8 with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.8.1.9 0.0029
-
 thioredoxin 8 with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.8.1.9 0.0036
-
 thioredoxin 41 with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.8.1.9 0.0047
-
 thioredoxin with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.8.1.9 0.0062
-
 thioredoxin with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.8.1.9 0.023
-
 NADH with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.8.1.9 1.1
-
 5,5'-dithiobis(2-nitrobenzoic acid) with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.8.1.9 1.4
-
 5,5'-dithiobis(2-nitrobenzoic acid) with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.9 64000
-
 2 * 64000, SDS-PAGE Entamoeba histolytica
1.8.1.9 78000
-
 gel filtration Entamoeba histolytica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.9 additional information Entamoeba histolytica in addition, the enzyme exhibits NAD(P)H dependent oxidase activity, which generates hydrogen peroxide from molecular oxygen ?
-
 ?
1.8.1.9 thioredoxin + NADP+ Entamoeba histolytica
-
 thioredoxin disulfide + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Entamoeba histolytica
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADH + H+
-
 Entamoeba histolytica 5'-thionitrobenzoic acid + NAD+
-
 ?
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
 Entamoeba histolytica 5'-thionitrobenzoic acid + NADP+
-
 ?
1.8.1.9 additional information in addition, the enzyme exhibits NAD(P)H dependent oxidase activity, which generates hydrogen peroxide from molecular oxygen Entamoeba histolytica ?
-
 ?
1.8.1.9 thioredoxin + NADP+
-
 Entamoeba histolytica thioredoxin disulfide + NADPH + H+
-
 ?
1.8.1.9 thioredoxin 41 + NADP+
-
 Entamoeba histolytica thioredoxin 41 disulfide + NADPH + H+
-
 ?
1.8.1.9 thioredoxin 8 + NADP+
-
 Entamoeba histolytica thioredoxin 8 disulfide + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.9 homodimer 2 * 64000, SDS-PAGE Entamoeba histolytica

Synonyms

EC Number Synonyms Comment Organism
1.8.1.9 TrxR
-
 Entamoeba histolytica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.9 0.2
-
 NADH with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.8.1.9 0.23
-
 5,5'-dithiobis(2-nitrobenzoic acid) with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.8.1.9 0.25
-
 NADPH with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.8.1.9 0.25
-
 5,5'-dithiobis(2-nitrobenzoic acid) with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.8.1.9 0.5
-
 thioredoxin with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.8.1.9 1.25
-
 thioredoxin with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.8.1.9 2
-
 thioredoxin 41 with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.8.1.9 2.2
-
 thioredoxin 41 with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.8.1.9 2.6
-
 thioredoxin 8 with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.8.1.9 2.7
-
 thioredoxin 8 with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.9 FAD 1 mol FAD is bound per mol subunit Entamoeba histolytica
1.8.1.9 NADH
-
 Entamoeba histolytica
1.8.1.9 NADPH higher affinity and catalytic efficiency with NADPH compared to NADH Entamoeba histolytica

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8.1.9 0.007
-
 NADP+ in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.8.1.9 0.026
-
 NADP+ in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.8.1.9 0.313
-
 NAD+ in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.8.1.9 1.478
-
 NAD+ in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.8.1.9 0.0025
-
 in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica Cu2+
1.8.1.9 0.0034
-
 in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica Zn2+

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8.1.9 0.17
-
 5,5'-dithiobis(2-nitrobenzoic acid) with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.8.1.9 0.23
-
 5,5'-dithiobis(2-nitrobenzoic acid) with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.8.1.9 75
-
 thioredoxin with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.8.1.9 84
-
 NADH with 5,5'-dithiobis(2-nitrobenzoicacid) as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.8.1.9 140
-
 NADPH with 5,5'-dithiobis(2-nitrobenzoic acid) as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.8.1.9 260
-
 thioredoxin with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.8.1.9 600
-
 thioredoxin 41 with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.8.1.9 880
-
 thioredoxin 41 with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.8.1.9 890
-
 thioredoxin 8 with NADH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica
1.8.1.9 950
-
 thioredoxin 8 with NADPH as cosubstrate, in 50 mM potassium phosphate, pH 7.0, 2 mM EDTA at 30°C Entamoeba histolytica