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Literature summary extracted from

  • Crichton, P.; Albury, M.; Affourtit, C.; Moore, A.
    Mutagenesis of the Sauromatum guttatum alternative oxidase reveals features important for oxygen binding and catalysis (2010), Biochim. Biophys. Acta, 1797, 732-737.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.11 expression of wild-type and mutant enzymes in Schizosaccharomyces pombe strain Sp.011, subcloning in Escherichia coli strains JM101 and 110 Sauromatum venosum

Protein Variants

EC Number Protein Variants Comment Organism
1.10.3.11 C172A site-directed mutagenesis, the mutant shows reduced activity and oxygen affinity compared to the wild-type enzyme Sauromatum venosum
1.10.3.11 T179A site-directed mutagenesis, the mutant shows reduced activity and oxygen affinity compared to the wild-type enzyme Sauromatum venosum
1.10.3.11 W206F site-directed mutagenesis, inactive mutant Sauromatum venosum
1.10.3.11 W206Y site-directed mutagenesis, inactive mutant Sauromatum venosum
1.10.3.11 Y253F site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Sauromatum venosum
1.10.3.11 Y299F site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Sauromatum venosum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.11 octyl gallate
-
Sauromatum venosum
1.10.3.11 sialic acid
-
Sauromatum venosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.11 0.0046
-
O2 mutant T179A, pH and temperature not specified in the publication Sauromatum venosum
1.10.3.11 0.0092
-
O2 mutant C172A, pH and temperature not specified in the publication Sauromatum venosum
1.10.3.11 0.017
-
O2 mutant Y299F, pH and temperature not specified in the publication Sauromatum venosum
1.10.3.11 0.018
-
O2 wild-type enzyme, pH and temperature not specified in the publication Sauromatum venosum
1.10.3.11 0.0205
-
O2 mutant Y253F, pH and temperature not specified in the publication Sauromatum venosum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.11 mitochondrion
-
Sauromatum venosum 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.10.3.11 2 ubiquinol + O2 Sauromatum venosum
-
2 ubiquinone + 2 H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.11 Sauromatum venosum P22185
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.11 2 ubiquinol + O2
-
Sauromatum venosum 2 ubiquinone + 2 H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.10.3.11 alternative oxidase
-
Sauromatum venosum
1.10.3.11 AOX
-
Sauromatum venosum

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.10.3.11 0.00007
-
mutant Y299F, pH and temperature not specified in the publication Sauromatum venosum octyl gallate
1.10.3.11 0.00008
-
mutants C172A and T179A, pH and temperature not specified in the publication Sauromatum venosum octyl gallate
1.10.3.11 0.00009
-
wild-type enzyme, pH and temperature not specified in the publication Sauromatum venosum octyl gallate
1.10.3.11 0.0001
-
mutant Y253F, pH and temperature not specified in the publication Sauromatum venosum octyl gallate
1.10.3.11 0.012
-
mutant T179A, pH and temperature not specified in the publication Sauromatum venosum sialic acid
1.10.3.11 0.016
-
wild-type enzyme, pH and temperature not specified in the publication Sauromatum venosum sialic acid
1.10.3.11 0.018
-
mutant Y299F, pH and temperature not specified in the publication Sauromatum venosum sialic acid
1.10.3.11 0.019
-
mutant C172A, pH and temperature not specified in the publication Sauromatum venosum sialic acid
1.10.3.11 0.02
-
mutant Y253F, pH and temperature not specified in the publication Sauromatum venosum sialic acid

General Information

EC Number General Information Comment Organism
1.10.3.11 evolution the enzyme is found in mitochondria of all higher plants studied to date Sauromatum venosum
1.10.3.11 physiological function the alternative oxidase (AOX) is a non-protonmotive ubiquinol oxidase Sauromatum venosum