EC Number | Cloned (Comment) | Organism |
---|---|---|
1.10.3.11 | expression of wild-type and mutant enzymes in Schizosaccharomyces pombe strain Sp.011, subcloning in Escherichia coli strains JM101 and 110 | Sauromatum venosum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.10.3.11 | C172A | site-directed mutagenesis, the mutant shows reduced activity and oxygen affinity compared to the wild-type enzyme | Sauromatum venosum |
1.10.3.11 | T179A | site-directed mutagenesis, the mutant shows reduced activity and oxygen affinity compared to the wild-type enzyme | Sauromatum venosum |
1.10.3.11 | W206F | site-directed mutagenesis, inactive mutant | Sauromatum venosum |
1.10.3.11 | W206Y | site-directed mutagenesis, inactive mutant | Sauromatum venosum |
1.10.3.11 | Y253F | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Sauromatum venosum |
1.10.3.11 | Y299F | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Sauromatum venosum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.10.3.11 | octyl gallate | - |
Sauromatum venosum | |
1.10.3.11 | sialic acid | - |
Sauromatum venosum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.10.3.11 | 0.0046 | - |
O2 | mutant T179A, pH and temperature not specified in the publication | Sauromatum venosum | |
1.10.3.11 | 0.0092 | - |
O2 | mutant C172A, pH and temperature not specified in the publication | Sauromatum venosum | |
1.10.3.11 | 0.017 | - |
O2 | mutant Y299F, pH and temperature not specified in the publication | Sauromatum venosum | |
1.10.3.11 | 0.018 | - |
O2 | wild-type enzyme, pH and temperature not specified in the publication | Sauromatum venosum | |
1.10.3.11 | 0.0205 | - |
O2 | mutant Y253F, pH and temperature not specified in the publication | Sauromatum venosum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.10.3.11 | mitochondrion | - |
Sauromatum venosum | 5739 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.10.3.11 | 2 ubiquinol + O2 | Sauromatum venosum | - |
2 ubiquinone + 2 H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.10.3.11 | Sauromatum venosum | P22185 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.10.3.11 | 2 ubiquinol + O2 | - |
Sauromatum venosum | 2 ubiquinone + 2 H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.10.3.11 | alternative oxidase | - |
Sauromatum venosum |
1.10.3.11 | AOX | - |
Sauromatum venosum |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
1.10.3.11 | 0.00007 | - |
mutant Y299F, pH and temperature not specified in the publication | Sauromatum venosum | octyl gallate | |
1.10.3.11 | 0.00008 | - |
mutants C172A and T179A, pH and temperature not specified in the publication | Sauromatum venosum | octyl gallate | |
1.10.3.11 | 0.00009 | - |
wild-type enzyme, pH and temperature not specified in the publication | Sauromatum venosum | octyl gallate | |
1.10.3.11 | 0.0001 | - |
mutant Y253F, pH and temperature not specified in the publication | Sauromatum venosum | octyl gallate | |
1.10.3.11 | 0.012 | - |
mutant T179A, pH and temperature not specified in the publication | Sauromatum venosum | sialic acid | |
1.10.3.11 | 0.016 | - |
wild-type enzyme, pH and temperature not specified in the publication | Sauromatum venosum | sialic acid | |
1.10.3.11 | 0.018 | - |
mutant Y299F, pH and temperature not specified in the publication | Sauromatum venosum | sialic acid | |
1.10.3.11 | 0.019 | - |
mutant C172A, pH and temperature not specified in the publication | Sauromatum venosum | sialic acid | |
1.10.3.11 | 0.02 | - |
mutant Y253F, pH and temperature not specified in the publication | Sauromatum venosum | sialic acid |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.10.3.11 | evolution | the enzyme is found in mitochondria of all higher plants studied to date | Sauromatum venosum |
1.10.3.11 | physiological function | the alternative oxidase (AOX) is a non-protonmotive ubiquinol oxidase | Sauromatum venosum |