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Literature summary extracted from

  • Serrano, H.; Blanchard, J.S.
    Kinetic and isotopic characterization of L-proline dehydrogenase from Mycobacterium tuberculosis (2013), Biochemistry, 52, 5009-5015.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.5.5.2 K110A inactive Mycobacterium tuberculosis
1.5.5.2 Y203F the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.5.2 0.0034
-
 2,6-dichlorophenolindophenol wild type enzyme, at 25°C and pH 7.1 in 20 mM Tris-HCl Mycobacterium tuberculosis
1.5.5.2 0.86
-
 L-proline mutant enzyme Y203F, at 25°C and pH 7.1 in 20 mM Tris-HCl Mycobacterium tuberculosis
1.5.5.2 5.7
-
 L-proline wild type enzyme, at 25°C and pH 7.1 in 20 mM Tris-HCl Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.5.2 37000
-
 2 * 37000, SDS-PAGE Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
1.5.5.2 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.5.2 nickel affinity column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.5.2 L-proline + 2,6-dichlorophenolindophenol + H2O
-
 Mycobacterium tuberculosis DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.5.2 homodimer 2 * 37000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
1.5.5.2 L-proline dehydrogenase
-
 Mycobacterium tuberculosis
1.5.5.2 PRODH
-
 Mycobacterium tuberculosis
1.5.5.2 prub
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.5.2 33.5
-
 L-proline wild type enzyme, at 25°C and pH 7.1 in 20 mM Tris-HCl Mycobacterium tuberculosis
1.5.5.2 100.6
-
 L-proline mutant enzyme Y203F, at 25°C and pH 7.1 in 20 mM Tris-HCl Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.5.2 FAD
-
 Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5.5.2 0.117
-
 L-proline mutant enzyme Y203F, at 25°C and pH 7.1 in 20 mM Tris-HCl Mycobacterium tuberculosis
1.5.5.2 5.877
-
 L-proline wild type enzyme, at 25°C and pH 7.1 in 20 mM Tris-HCl Mycobacterium tuberculosis