Literature summary extracted from
Asciutto, E.K.; Young, M.J.; Madura, J.; Pochapsky, S.S.; Pochapsky, T.C.
Solution structural ensembles of substrate-free cytochrome P450(cam) (2012), Biochemistry, 51, 3383-3393.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.14.15.1 |
expression of C334A enzyme mutant in Escherichia coli strain NCM533 |
Pseudomonas putida |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.14.15.1 |
C334A |
site-directed mutagenesis, the C334A mutant is spectroscopically and enzymatically identical to the wild type but does not form dimers in solution, and so is more suitable for NMR structure analysis than the wild type enzyme |
Pseudomonas putida |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.14.15.1 |
Fe2+ |
cytochrome P450 containing enzyme |
Pseudomonas putida |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.15.1 |
Pseudomonas putida |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.14.15.1 |
recombinant C334A enzyme mutant from Escherichia coli strain NCM533 by ammonium sulfate fractionation, dialysis, anion exchange chromatography, and gel filtration |
Pseudomonas putida |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.14.15.1 |
(+)-camphor + reduced putidaredoxin + O2 |
- |
Pseudomonas putida |
(+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.14.15.1 |
CYP101A1 |
- |
Pseudomonas putida |
1.14.15.1 |
cytochrome p450cam |
- |
Pseudomonas putida |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.14.15.1 |
7.4 |
- |
assay at |
Pseudomonas putida |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.14.15.1 |
cytochrome P450 |
cytochrome P450cam |
Pseudomonas putida |
|
1.14.15.1 |
putidaredoxin |
- |
Pseudomonas putida |
|
General Information
EC Number |
General Information |
Comment |
Organism |
---|
1.14.15.1 |
additional information |
NMR structure analysis of the enzyme CYP101A1 with substrate (+)-camphor bound the active site and substrate-free enzyme after removal of (+)-camphor, molecular dynamics simulations and modeling, overview. Portions of a beta-rich region adjacent to the active site shift so as to partially occupy the vacancy left by removal of substrate. The accessible volume of the active site is reduced in the substrate-free enzyme relative to the substrate-bound structure |
Pseudomonas putida |