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Literature summary extracted from

  • Bera, A.K.; Atanasova, V.; Dhanda, A.; Ladner, J.E.; Parsons, J.F.
    Structure of aminodeoxychorismate synthase from Stenotrophomonas maltophilia (2012), Biochemistry, 51, 10208-10217.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.85 pabB, optimized for overexpression in Escherichia coli, His-tagged protein expressed in Escherichia coli BL21(DE3) Stenotrophomonas maltophilia

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.85 pH 7.8, polyethylene glycol monomethylether 550 Stenotrophomonas maltophilia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.85 0.24
-
 chorismate PabB, pH 8.0, 25°C Stenotrophomonas maltophilia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.85 49500
-
 PabB Stenotrophomonas maltophilia

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.85 Stenotrophomonas maltophilia
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.85 immobilized metal ion affinity chromatography (Co2+), fusion tag removed Stenotrophomonas maltophilia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.85 2-amino-2-deoxychorismate + NH3 PabB, second half of the reaction, starting with the intermediate Stenotrophomonas maltophilia 4-amino-4-deoxychorismate + NH3
-
 ?
2.6.1.85 chorismate + NH3 PabB, intermediate 2-amino-2-deoxychorismate involved Stenotrophomonas maltophilia 4-amino-4-deoxychorismate + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.85 monomer PabB in solution Stenotrophomonas maltophilia

Synonyms

EC Number Synonyms Comment Organism
2.6.1.85 aminodeoxychorismate synthase heterodimeric complex, subunit PabB catalyces the amination of chorismate, subunit PabA is an amidotransferase that supplies ammonia from glutamine hydrolysis, subunit PabA is not identfied in Stenotrophomonas maltophilia Stenotrophomonas maltophilia

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.85 0.007
-
 chorismate PabB, addition of Escherichia coli PabA enhances the reaction rate 20fold in the absence of glutamine, pH 8.0, 25°C Stenotrophomonas maltophilia
2.6.1.85 0.02
-
 2-amino-2-deoxychorismate PabB, second half of the reaction, pH 8.0, 25°C Stenotrophomonas maltophilia