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Literature summary extracted from

  • Young, A.P.; Bandarian, V.
    Pyruvate is the source of the two carbons that are required for formation of the imidazoline ring of 4-demethylwyosine (2011), Biochemistry, 50, 10573-10575.
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.44 N1-methylguanine37 in tRNAPhe + pyruvate + S-adenosyl-L-methionine Methanocaldococcus jannaschii enzyme catalyzes the condensation of N-methylguanosine with two carbon atoms from pyruvate to form 4-demethylwyosine, which is a common intermediate in the biosynthesis of all of the hypermodified RNA bases related to wybutosine. C2 and C3 of pyruvate are incorporated while C1 is not 4-demethylwyosine37 in tRNAPhe + L-methionine + 5'-deoxyadenosine + CO2 + H2O
-
 ?
4.1.3.44 N1-methylguanine37 in tRNAPhe + pyruvate + S-adenosyl-L-methionine Methanocaldococcus jannaschii ATCC 43067 enzyme catalyzes the condensation of N-methylguanosine with two carbon atoms from pyruvate to form 4-demethylwyosine, which is a common intermediate in the biosynthesis of all of the hypermodified RNA bases related to wybutosine. C2 and C3 of pyruvate are incorporated while C1 is not 4-demethylwyosine37 in tRNAPhe + L-methionine + 5'-deoxyadenosine + CO2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.44 Methanocaldococcus jannaschii Q57705
-
-
4.1.3.44 Methanocaldococcus jannaschii ATCC 43067 Q57705
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.44 purified anaerobically and reconstituted with Fe and S. The reconstituted protein is brown and has an absorbance spectrum that displays a characteristic shoulder at 400 nm Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.44 additional information the conserved Lys residue forms a Schiff base with pyruvate. Reductive cleavage of SAM leads to formation of dAdo radical, which propagates the radical to the m1G generating a substrate radical. Radical addition to C-2 of pyruvate followed by homolytic scission of the C-1-C-2 bond would generate an intermediate, which through transimmination and subsequent deprotonation, forms imG-14. The resulting formyl radical can either acquire an H-atom or undergo reduction and protonation to produce formate. Alternatively, oxidation of the formyl radical would generate CO2 Methanocaldococcus jannaschii ?
-
 ?
4.1.3.44 additional information the conserved Lys residue forms a Schiff base with pyruvate. Reductive cleavage of SAM leads to formation of dAdo radical, which propagates the radical to the m1G generating a substrate radical. Radical addition to C-2 of pyruvate followed by homolytic scission of the C-1-C-2 bond would generate an intermediate, which through transimmination and subsequent deprotonation, forms imG-14. The resulting formyl radical can either acquire an H-atom or undergo reduction and protonation to produce formate. Alternatively, oxidation of the formyl radical would generate CO2 Methanocaldococcus jannaschii ATCC 43067 ?
-
 ?
4.1.3.44 N1-methylguanine37 in tRNAPhe + pyruvate + S-adenosyl-L-methionine enzyme catalyzes the condensation of N-methylguanosine with two carbon atoms from pyruvate to form 4-demethylwyosine, which is a common intermediate in the biosynthesis of all of the hypermodified RNA bases related to wybutosine. C2 and C3 of pyruvate are incorporated while C1 is not Methanocaldococcus jannaschii 4-demethylwyosine37 in tRNAPhe + L-methionine + 5'-deoxyadenosine + CO2 + H2O
-
 ?
4.1.3.44 N1-methylguanine37 in tRNAPhe + pyruvate + S-adenosyl-L-methionine enzyme catalyzes the condensation of N-methylguanosine with two carbon atoms from pyruvate to form 4-demethylwyosine, which is a common intermediate in the biosynthesis of all of the hypermodified RNA bases related to wybutosine. C2 and C3 of pyruvate are incorporated while C1 is not Methanocaldococcus jannaschii ATCC 43067 4-demethylwyosine37 in tRNAPhe + L-methionine + 5'-deoxyadenosine + CO2 + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.3.44 Taw1
-
 Methanocaldococcus jannaschii
4.1.3.44 tRNA wyosine derivatives biosynthesis protein Taw1
-
 Methanocaldococcus jannaschii
4.1.3.44 TYW1
-
 Methanocaldococcus jannaschii