Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Willis, R.M.; Wahlen, B.D.; Seefeldt, L.C.; Barney, B.M.
    Characterization of a fatty acyl-CoA reductase from Marinobacter aquaeolei VT8: a bacterial enzyme catalyzing the reduction of fatty acyl-CoA to fatty alcohol (2011), Biochemistry, 50, 10550-10558.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.42 expressed in Escherichia coli strain TB1 Marinobacter nauticus
1.2.1.50 plasmid pPCRMALD8 containing the FACoAR from Marinobacter aquaeolei VT8 with an N-terminal maltose binding protein fusion and a C-terminal His-tag transformed into the Escherichia coli TB1 strain for protein expression Marinobacter nauticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.42 0.004
-
 hexadecanoyl-CoA at pH 7.0 and 37°C Marinobacter nauticus
1.2.1.42 0.05
-
 cis-11-hexadecenal at pH 7.0 and 37°C Marinobacter nauticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.42 116000
-
 x * 116000, SDS-PAGE Marinobacter nauticus
1.2.1.50 116000
-
 SDS-PAGE, in agreement with mass calculated from amino acid sequence Marinobacter nauticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.42 arachidonoyl-CoA + NADPH + H+ Marinobacter nauticus 86% activity compared to hexadecanoyl-CoA arachidonic aldehyde + CoA + NADP+
-
 ?
1.2.1.42 cis-11-hexadecenal + CoA + NADP+ Marinobacter nauticus
-
 (E)-hexadec-11-enoyl-CoA + NADPh + H+
-
 ?
1.2.1.42 dodecanoyl-CoA + NADPH + H+ Marinobacter nauticus 26% activity compared to hexadecanoyl-CoA dodecanal + CoA + NADP+
-
 ?
1.2.1.42 hexadecanoyl-CoA + NADPH + H+ Marinobacter nauticus 100% activity 1-hexadecanal + CoA + NADP+
-
 ?
1.2.1.42 additional information Marinobacter nauticus the enzyme exhibits no detectable reduction with free fatty acids, less than 1% activity with acetaldehyde and propanal ?
-
 ?
1.2.1.42 octadecanoyl-CoA + NADPH + H+ Marinobacter nauticus 69% activity compared to hexadecanoyl-CoA octadecanal + CoA + NADP+
-
 ?
1.2.1.42 octanoyl-CoA + NADPH + H+ Marinobacter nauticus 9% activity compared to hexadecanoyl-CoA octanal + CoA + NADP+
-
 ?
1.2.1.42 oleoyl-CoA + NADPH + H+ Marinobacter nauticus 78% activity compared to hexadecanoyl-CoA oleic aldehyde + CoA + NADP+
-
 ?
1.2.1.42 palmitoleoyl-CoA + NADPH + H+ Marinobacter nauticus 99% activity compared to hexadecanoyl-CoA palmitoleic aldehyde + CoA + NADP+
-
 ?
1.2.1.42 tetradecanoyl-CoA + NADPH + H+ Marinobacter nauticus 60% activity compared to hexadecanoyl-CoA tetradecanal + CoA + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.42 Marinobacter nauticus
-
-
-
1.2.1.50 Marinobacter nauticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.42 amylose column chromatography, metal affinity column chromatography, and G25 Sephadex gel filtration Marinobacter nauticus
1.2.1.50 by amylose affinity chromatography and metal affinity chromatography with nickel Marinobacter nauticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.42 0.015
-
 using octanoyl-CoA as substrate, at pH 7.0 and 37°C Marinobacter nauticus
1.2.1.42 0.034
-
 using dodecanoyl-CoA as substrate, at pH 7.0 and 37°C Marinobacter nauticus
1.2.1.42 0.04
-
 using octadecanoyl-CoA as substrate, at pH 7.0 and 37°C Marinobacter nauticus
1.2.1.42 0.044
-
 using oleoyl-CoA as substrate, at pH 7.0 and 37°C Marinobacter nauticus
1.2.1.42 0.049
-
 using arachidonoyl-CoA as substrate, at pH 7.0 and 37°C Marinobacter nauticus
1.2.1.42 0.057
-
 using hexadecanoyl-CoA as substrate, at pH 7.0 and 37°C Marinobacter nauticus
1.2.1.42 0.057
-
 using palmitoleyl-CoA as substrate, at pH 7.0 and 37°C Marinobacter nauticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.42 arachidonoyl-CoA + NADPH + H+ 86% activity compared to hexadecanoyl-CoA Marinobacter nauticus arachidonic aldehyde + CoA + NADP+
-
 ?
1.2.1.42 cis-11-hexadecenal + CoA + NADP+
-
 Marinobacter nauticus (E)-hexadec-11-enoyl-CoA + NADPh + H+
-
 ?
1.2.1.42 dodecanoyl-CoA + NADPH + H+ 26% activity compared to hexadecanoyl-CoA Marinobacter nauticus dodecanal + CoA + NADP+
-
 ?
1.2.1.42 hexadecanoyl-CoA + NADPH + H+ 100% activity Marinobacter nauticus 1-hexadecanal + CoA + NADP+
-
 ?
1.2.1.42 additional information the enzyme exhibits no detectable reduction with free fatty acids, less than 1% activity with acetaldehyde and propanal Marinobacter nauticus ?
-
 ?
1.2.1.42 octadecanoyl-CoA + NADPH + H+ 69% activity compared to hexadecanoyl-CoA Marinobacter nauticus octadecanal + CoA + NADP+
-
 ?
1.2.1.42 octanoyl-CoA + NADPH + H+ 9% activity compared to hexadecanoyl-CoA Marinobacter nauticus octanal + CoA + NADP+
-
 ?
1.2.1.42 oleoyl-CoA + NADPH + H+ 78% activity compared to hexadecanoyl-CoA Marinobacter nauticus oleic aldehyde + CoA + NADP+
-
 ?
1.2.1.42 palmitoleoyl-CoA + NADPH + H+ 99% activity compared to hexadecanoyl-CoA Marinobacter nauticus palmitoleic aldehyde + CoA + NADP+
-
 ?
1.2.1.42 tetradecanoyl-CoA + NADPH + H+ 60% activity compared to hexadecanoyl-CoA Marinobacter nauticus tetradecanal + CoA + NADP+
-
 ?
1.2.1.50 arachidonoyl-CoA + NADPH + H+
-
 Marinobacter nauticus (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenal + CoA + NADP+ further reduction of (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenal into the corresponding (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraen-1-ol ?
1.2.1.50 fatty acyl-CoA + NADPH + H+
-
 Marinobacter nauticus fatty aldehyde + CoA + NADP+ reaction intermediate, enzyme converts fatty acyl-CoA directly into the corresponding fatty alcohol by four-electron reduction, free fatty aldehyde intermediate is not accessible ?
1.2.1.50 lauroyl-CoA + NADPH + H+
-
 Marinobacter nauticus dodecanal + CoA + NADP+ further reduction of dodecanal into the corresponding dodecan-1-ol ?
1.2.1.50 additional information no reaction product detected with NADH or free fatty acids Marinobacter nauticus ?
-
 ?
1.2.1.50 myristoyl-CoA + NADPH + H+
-
 Marinobacter nauticus tetradecanal + CoA + NADP+ further reduction of tetradecanal into the corresponding tetradecan-1-ol ?
1.2.1.50 octanoyl-CoA + NADPH + H+
-
 Marinobacter nauticus octanal + NADP+ further reduction of octanal into the corresponding octan-1-ol ?
1.2.1.50 oleoyl-CoA + NADPH + H+
-
 Marinobacter nauticus (9Z)-octadec-9-enal + CoA + NADP+ further reduction of (9Z)-octadec-9-enal into the corresponding (9Z)-octadec-9-en-1-ol ?
1.2.1.50 palmitoleoyl-CoA + NADPH + H+
-
 Marinobacter nauticus (9Z)-hexadec-9-enal + CoA + NADP+ further reduction of (9Z)-hexadec-9-enal into the corresponding (9Z)-hexadec-9-en-1-ol ?
1.2.1.50 palmitoyl-CoA + NADPH + H+
-
 Marinobacter nauticus hexadecanal + CoA + NADP+ further reduction of hexadecanal into the corresponding hexadecan-1-ol ?
1.2.1.50 stearoyl-CoA + NADPH + H+
-
 Marinobacter nauticus octadecanal + CoA + NADP+ further reduction of octadecanal into the corresponding octadecan-1-ol ?

Subunits

EC Number Subunits Comment Organism
1.2.1.42 ? x * 116000, SDS-PAGE Marinobacter nauticus

Synonyms

EC Number Synonyms Comment Organism
1.2.1.42 FACoAR
-
 Marinobacter nauticus
1.2.1.42 fatty acyl-CoA reductase
-
 Marinobacter nauticus
1.2.1.50 FACoAR
-
 Marinobacter nauticus
1.2.1.50 fatty acyl-CoA reductase
-
 Marinobacter nauticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.42 7
-
-
 Marinobacter nauticus
1.2.1.50 7
-
-
 Marinobacter nauticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.42 6.5 9 optimal activity is at pH 7.0, with activity falling dramatically below pH 6.5 and above 9.0 Marinobacter nauticus
1.2.1.50 6.5 9 activity falling dramatically below pH 6.5 and above 9.0 Marinobacter nauticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.42 NADPH
-
 Marinobacter nauticus