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Literature summary extracted from

  • Chocklett, S.W.; Sobrado, P.
    Aspergillus fumigatus SidA is a highly specific ornithine hydroxylase with bound flavin cofactor (2010), Biochemistry, 49, 6777-6783.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.196 expressed as a fusion to MBP containing an 8His tag at the N-terminus in Escherichia coli BL21T1R cells Aspergillus fumigatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.196 NADH substrate inhibition Aspergillus fumigatus
1.14.13.196 NADP+ competitive inhibitor with respect to NADPH Aspergillus fumigatus
1.14.13.196 NADPH substrate inhibition Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.196 0.03
-
 NADPH pH 7.5, 25°C, oxygen consumption assay Aspergillus fumigatus
1.14.13.196 0.21
-
 NADH pH 7.5, 25°C, oxygen consumption assay Aspergillus fumigatus
1.14.13.196 0.5
-
 L-ornithine pH 7.5, 25°C, cosubstrate: NADH, oxygen consumption assay Aspergillus fumigatus
1.14.13.196 0.9
-
 NADPH pH 7.5, 25°C, following the formation of hydroxylated ornithine Aspergillus fumigatus
1.14.13.196 0.94
-
 NADH pH 7.5, 25°C, following the formation of hydroxylated ornithine Aspergillus fumigatus
1.14.13.196 1.7
-
 L-ornithine pH 7.5, 25°C, cosubstrate: NADH, following the formation of hydroxylated ornithine Aspergillus fumigatus
1.14.13.196 1.7
-
 L-ornithine pH 7.5, 25°C, cosubstrate: NADPH, following the formation of hydroxylated ornithine Aspergillus fumigatus
1.14.13.196 2
-
 L-ornithine pH 7.5, 25°C, cosubstrate: NADPH, oxygen consumption assay Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.196 55000
-
 4 * 55000, SDS-PAGE Aspergillus fumigatus
1.14.13.196 57210
-
 4 * 57210, MALDI-TOF MS Aspergillus fumigatus
1.14.13.196 228000
-
 gel filtration Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.196 L-ornithine + NAD(P)H + H+ + O2 Aspergillus fumigatus the enzyme catalyzes the hydroxylation of L-ornithine in ferrichrome biosynthesis N5-hydroxy-L-ornithine + NAD(P)+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.196 Aspergillus fumigatus Q5SE95
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.196
-
 Aspergillus fumigatus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.13.196 L-ornithine + NAD(P)H + H+ + O2 = N5-hydroxy-L-ornithine + NAD(P)+ + H2O the enzyme forms a ternary complex with NADP and L-ornithine during catalysis. The enzyme likely proceeds by a sequential kinetic mechanism Aspergillus fumigatus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.196 L-ornithine + NAD(P)H + H+ + O2 the enzyme catalyzes the hydroxylation of L-ornithine in ferrichrome biosynthesis Aspergillus fumigatus N5-hydroxy-L-ornithine + NAD(P)+ + H2O
-
 ?
1.14.13.196 L-ornithine + NADH + H+ + O2 AfSidA is highly specific for its amino acid substrate, only hydroxylating L-ornithine. An 8-fold preference in the catalytic efficiency is determined for NADPH compared to NADH Aspergillus fumigatus N5-hydroxy-L-ornithine + NAD+ + H2O
-
 ?
1.14.13.196 L-ornithine + NADPH + H+ + O2 AfSidA is highly specific for its amino acid substrate, only hydroxylating L-ornithine. An 8-fold preference in the catalytic efficiency is determined for NADPH compared to NADH Aspergillus fumigatus N5-hydroxy-L-ornithine + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.196 tetramer 4 * 55000, SDS-PAGE Aspergillus fumigatus
1.14.13.196 tetramer 4 * 57210, MALDI-TOF MS Aspergillus fumigatus

Synonyms

EC Number Synonyms Comment Organism
1.14.13.196 AfSidA ambiguous Aspergillus fumigatus
1.14.13.196 siderophore A
-
 Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.196 25
-
 assay at Aspergillus fumigatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.196 0.48
-
 L-ornithine pH 7.5, 25°C, cosubstrate: NADPH, following the formation of hydroxylated ornithine Aspergillus fumigatus
1.14.13.196 0.5
-
 L-ornithine pH 7.5, 25°C, cosubstrate: NADH, following the formation of hydroxylated ornithine Aspergillus fumigatus
1.14.13.196 0.63
-
 NADPH pH 7.5, 25°C, oxygen consumption assay Aspergillus fumigatus
1.14.13.196 0.68
-
 L-ornithine pH 7.5, 25°C, cosubstrate: NADH, oxygen consumption assay Aspergillus fumigatus
1.14.13.196 0.87
-
 L-ornithine pH 7.5, 25°C, cosubstrate: NADPH, oxygen consumption assay Aspergillus fumigatus
1.14.13.196 1.18
-
 NADH pH 7.5, 25°C, following the formation of hydroxylated ornithine Aspergillus fumigatus
1.14.13.196 1.25
-
 NADPH pH 7.5, 25°C, following the formation of hydroxylated ornithine Aspergillus fumigatus
1.14.13.196 1.36
-
 NADH pH 7.5, 25°C, oxygen consumption assay Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.196 7.5
-
 assay at Aspergillus fumigatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.196 FAD flavoprotein Aspergillus fumigatus
1.14.13.196 NADH 8-fold preference in the catalytic efficiency is determined for NADPH compared to NADH Aspergillus fumigatus
1.14.13.196 NADPH 8-fold preference in the catalytic efficiency is determined for NADPH compared to NADH Aspergillus fumigatus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.13.196 1.9
-
 NADH pH 7.5, 25°C Aspergillus fumigatus
1.14.13.196 2.8
-
 NADPH pH 7.5, 25°C Aspergillus fumigatus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.13.196 0.28
-
 L-ornithine pH 7.5, 25°C, cosubstrate: NADH, following the formation of hydroxylated ornithine Aspergillus fumigatus
1.14.13.196 0.29
-
 L-ornithine pH 7.5, 25°C, cosubstrate: NADPH, following the formation of hydroxylated ornithine Aspergillus fumigatus
1.14.13.196 0.43
-
 L-ornithine pH 7.5, 25°C, cosubstrate: NADH, oxygen consumption assay Aspergillus fumigatus
1.14.13.196 1.3
-
 NADPH pH 7.5, 25°C, following the formation of hydroxylated ornithine Aspergillus fumigatus
1.14.13.196 1.3
-
 NADH pH 7.5, 25°C, following the formation of hydroxylated ornithine Aspergillus fumigatus
1.14.13.196 1.33
-
 L-ornithine pH 7.5, 25°C, cosubstrate: NADPH, oxygen consumption assay Aspergillus fumigatus
1.14.13.196 3.4
-
 NADH pH 7.5, 25°C, oxygen consumption assay Aspergillus fumigatus
1.14.13.196 21.05
-
 NADPH pH 7.5, 25°C, oxygen consumption assay Aspergillus fumigatus