Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sasaki, D.; Watanabe, S.; Kanai, T.; Atomi, H.; Imanaka, T.; Miki, K.
    Characterization and in vitro interaction study of a [NiFe] hydrogenase large subunit from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1 (2012), Biochem. Biophys. Res. Commun., 417, 192-196.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.12.99.6 expression in Escherichia coli Thermococcus kodakarensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.99.6 cytosol
-
 Thermococcus kodakarensis 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.99.6 Iron contains 0.23 Fe atoms per molecule of large subunit HyhL Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.99.6 48300
-
 and 96600, gel filtration Thermococcus kodakarensis
1.12.99.6 96600
-
 and 48300, gel filtration Thermococcus kodakarensis

Organism

EC Number Organism UniProt Comment Textmining
1.12.99.6 Thermococcus kodakarensis Q8NKS2 alpha subunit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.99.6 cytosolic [NiFe] hydrogenase large subunit HyhL Thermococcus kodakarensis

Subunits

EC Number Subunits Comment Organism
1.12.99.6 dimer 2 * 48300, calculated, 2 * 48300, gel filtration. Enzyme exists in equilibrium between dimer and monomer Thermococcus kodakarensis
1.12.99.6 monomer 1 * 48300, calculated, 1 * 48300, gel filtration. Enzyme exists in equilibrium between dimer and monomer Thermococcus kodakarensis
1.12.99.6 More subunit HyhL forms a tight 1:1 binary complex with hydrogenase HypA and weakly interacts with HypC Thermococcus kodakarensis

Synonyms

EC Number Synonyms Comment Organism
1.12.99.6 HyhL large subunit Thermococcus kodakarensis
1.12.99.6 [NiFe] hydrogenase
-
 Thermococcus kodakarensis