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Literature summary extracted from
- Enzymatic synthesis of hexyl glycosides from lactose at low water activity and high temperature using hyperthermostable beta-glycosidases (2002), Biocatal. Biotransform., 20, 167-178.
No PubMed abstract available
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.2.1.B26 | synthesis | optimization of hexyl-beta-D-glycoside synthesis from lactose in hexanol at low water activity and high temperature. Compared to other beta-glycosidases in lactose conversion into alkyl glycoside, the enzyme shows high activity in a hexanol one-phase system and synthesized high yields of both hexyl-beta-D-galactoside and hexyl-beta-D-glucoside. Using 32 g/l lactose (93 mM), the enzyme synthesizes yields of 41% galactoside (38.1 mM) and 29% glucoside (27.0 mM) | Saccharolobus solfataricus |
| 3.2.1.B28 | synthesis | optimization of hexyl-beta-glycoside synthesis from lactose in hexanol at low water activity and high temperature. Compared to other beta-glycosidases in lactose conversion into alkyl glycoside, the enzyme shows high activity in a hexanol one-phase system and synthesized high yields of both hexyl-beta-galactoside and hexyl-beta-glucoside. The enzyme synthesizes yields of 63% galactoside (58.6mM) and 28% glucoside (26.1 mM) | Pyrococcus furiosus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.B26 | Saccharolobus solfataricus | P22498 | - |
- |
| 3.2.1.B26 | Saccharolobus solfataricus P2 | P22498 | - |
- |
| 3.2.1.B28 | Pyrococcus furiosus | Q51723 | - |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.B26 | 0.58 | - |
pH 6.5, 75°C, enzyme immobilized on XAD-4 | Saccharolobus solfataricus |
| 3.2.1.B26 | 0.59 | - |
pH 6.5, 75°C, enzyme immobilized on XAD-16 | Saccharolobus solfataricus |
| 3.2.1.B26 | 1.71 | - |
pH 6.5, 75°C, lyophilized enzyme | Saccharolobus solfataricus |
| 3.2.1.B26 | 2.22 | - |
pH 6.5, 75°C, addition of 1% (w/v) SDS | Saccharolobus solfataricus |
| 3.2.1.B26 | 3.53 | - |
pH 6.5, 75°C, enzyme immobilized on Celite | Saccharolobus solfataricus |
| 3.2.1.B28 | 0.83 | - |
pH 5.0, 90°C, addition of aerosol dioctyl sulfosuccinate | Pyrococcus furiosus |
| 3.2.1.B28 | 2.75 | - |
pH 5.0, 90°C, enzyme immobilized on XAD-4 | Pyrococcus furiosus |
| 3.2.1.B28 | 3.18 | - |
pH 5.0, 90°C, enzyme immobilized on XAD-16 | Pyrococcus furiosus |
| 3.2.1.B28 | 4.77 | - |
pH 5.0, 90°C, enzyme immobilized on Celite | Pyrococcus furiosus |
| 3.2.1.B28 | 13.04 | - |
pH 5.0, 90°C, lyophilized enzyme | Pyrococcus furiosus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.B26 | 4-nitrophenyl beta-D-galactoside | LacS shows 70% hydrolytic activity on nitrophenyl-beta-glucoside relative to the activity on nitrophenyl-beta-galactoside | Saccharolobus solfataricus | 4-nitrophenol + beta-D-galactose | - |
? |  |
| 3.2.1.B26 | 4-nitrophenyl beta-D-galactoside | LacS shows 70% hydrolytic activity on nitrophenyl-beta-glucoside relative to the activity on nitrophenyl-beta-galactoside | Saccharolobus solfataricus P2 | 4-nitrophenol + beta-D-galactose | - |
? | |
| 3.2.1.B26 | 4-nitrophenyl beta-D-glucoside | LacS shows 70% hydrolytic activity on nitrophenyl-beta-glucoside relative to the activity on nitrophenyl-beta-galactoside | Saccharolobus solfataricus | 4-nitrophenol + beta-D-glucose | - |
? | |
| 3.2.1.B26 | 4-nitrophenyl beta-D-glucoside | LacS shows 70% hydrolytic activity on nitrophenyl-beta-glucoside relative to the activity on nitrophenyl-beta-galactoside | Saccharolobus solfataricus P2 | 4-nitrophenol + beta-D-glucose | - |
? | |
| 3.2.1.B26 | additional information | the enzyme catalyzes the conversion of lactose to hexyl-beta-D-galactoside in hexanol (transgalactosylation reaction) | Saccharolobus solfataricus | ? | - |
? | |
| 3.2.1.B26 | additional information | the enzyme catalyzes the conversion of lactose to hexyl-beta-D-galactoside in hexanol (transgalactosylation reaction) | Saccharolobus solfataricus P2 | ? | - |
? | |
| 3.2.1.B28 | 4-nitrophenyl beta-D-galactoside | CelB has a beta-galactosidase activity of 61% of the beta-glucosidase activity | Pyrococcus furiosus | 4-nitrophenol + beta-D-galactose | - |
? | |
| 3.2.1.B28 | 4-nitrophenyl beta-D-glucoside | CelB has a beta-galactosidase activity of 61% of the beta-glucosidase activity | Pyrococcus furiosus | 4-nitrophenol + beta-D-glucose | - |
? | |
| 3.2.1.B28 | additional information | the enzyme catalyzes the conversion of lactose to hexyl-beta-galactoside in hexanol (transgalactosylation reaction) | Pyrococcus furiosus | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.B26 | LACS | - |
Saccharolobus solfataricus |
| 3.2.1.B26 | SSO3019 | locus name | Saccharolobus solfataricus |
| 3.2.1.B28 | CelB | - |
Pyrococcus furiosus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.B26 | 75 | - |
assay at | Saccharolobus solfataricus |
| 3.2.1.B28 | 90 | - |
assay at | Pyrococcus furiosus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.B26 | 6.5 | - |
assay at | Saccharolobus solfataricus |
| 3.2.1.B28 | 5 | - |
assay at | Pyrococcus furiosus |
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