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Literature summary extracted from

  • Seedorf, H.; Dreisbach, A.; Hedderich, R.; Shima, S.; Thauer, R.K.
    F420H2 oxidase (FprA) from Methanobrevibacter arboriphilus, a coenzyme F420-dependent enzyme involved in O2 detoxification (2004), Arch. Microbiol., 182, 126-137.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.3.22
-
 Methanothermobacter marburgensis
1.5.3.22
-
 Methanobrevibacter arboriphilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.3.22 additional information the reaction is not inhibited by cyanide Methanobrevibacter arboriphilus
1.5.3.22 additional information the reaction is not inhibited by cyanide Methanothermobacter marburgensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.22 0.002
-
 O2 pH 7.6, 37°C Methanothermobacter marburgensis
1.5.3.22 0.002
-
 O2 pH 7.6, 37°C Methanobrevibacter arboriphilus
1.5.3.22 0.03
-
 reduced coenzyme F420 pH 7.6, 37°C Methanothermobacter marburgensis
1.5.3.22 0.03
-
 reduced coenzyme F420 pH 7.6, 37°C Methanobrevibacter arboriphilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.3.22 Fe2+ the 45000 Da protein harbors a binuclear iron center Methanobrevibacter arboriphilus
1.5.3.22 Iron the 45000 Da protein harbors a binuclear iron center Methanothermobacter marburgensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.3.22 43000
-
 x * 43000, SDS-PAGE Methanothermobacter marburgensis
1.5.3.22 45000
-
 x * 45000, SDS-PAGE Methanobrevibacter arboriphilus
1.5.3.22 45300
-
 x * 45300, calculated from sequence Methanothermobacter marburgensis
1.5.3.22 46100
-
 x * 46100, calculated from sequence Methanobrevibacter arboriphilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.22 2 reduced coenzyme F420 + O2 Methanothermobacter marburgensis the enzyme is involved in O2 detoxification 2 oxidized coenzyme F420 + 2 H2O
-
 ?
1.5.3.22 2 reduced coenzyme F420 + O2 Methanobrevibacter arboriphilus the enzyme is involved in O2 detoxification 2 oxidized coenzyme F420 + 2 H2O
-
 ?
1.5.3.22 2 reduced coenzyme F420 + O2 Methanothermobacter marburgensis DSM 2133 the enzyme is involved in O2 detoxification 2 oxidized coenzyme F420 + 2 H2O
-
 ?
1.5.3.22 2 reduced coenzyme F420 + O2 Methanobrevibacter arboriphilus DSM Z 744 the enzyme is involved in O2 detoxification 2 oxidized coenzyme F420 + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.22 Methanobrevibacter arboriphilus B1A7S3
-
-
1.5.3.22 Methanobrevibacter arboriphilus DSM Z 744 B1A7S3
-
-
1.5.3.22 Methanothermobacter marburgensis Q50497
-
-
1.5.3.22 Methanothermobacter marburgensis DSM 2133 Q50497
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.22
-
 Methanothermobacter marburgensis
1.5.3.22
-
 Methanobrevibacter arboriphilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.3.22 0.4
-
 pH 7.6, 37°C, cell suspension Methanobrevibacter arboriphilus
1.5.3.22 55
-
 pH 7.6, 37°C, purified enzyme Methanobrevibacter arboriphilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.22 2 reduced coenzyme F420 + O2 the enzyme is involved in O2 detoxification Methanothermobacter marburgensis 2 oxidized coenzyme F420 + 2 H2O
-
 ?
1.5.3.22 2 reduced coenzyme F420 + O2 the enzyme is involved in O2 detoxification Methanobrevibacter arboriphilus 2 oxidized coenzyme F420 + 2 H2O
-
 ?
1.5.3.22 2 reduced coenzyme F420 + O2 the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor Methanothermobacter marburgensis 2 oxidized coenzyme F420 + 2 H2O
-
 ?
1.5.3.22 2 reduced coenzyme F420 + O2 the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor Methanobrevibacter arboriphilus 2 oxidized coenzyme F420 + 2 H2O
-
 ?
1.5.3.22 2 reduced coenzyme F420 + O2 the enzyme is involved in O2 detoxification Methanothermobacter marburgensis DSM 2133 2 oxidized coenzyme F420 + 2 H2O
-
 ?
1.5.3.22 2 reduced coenzyme F420 + O2 the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor Methanothermobacter marburgensis DSM 2133 2 oxidized coenzyme F420 + 2 H2O
-
 ?
1.5.3.22 2 reduced coenzyme F420 + O2 the enzyme is involved in O2 detoxification Methanobrevibacter arboriphilus DSM Z 744 2 oxidized coenzyme F420 + 2 H2O
-
 ?
1.5.3.22 2 reduced coenzyme F420 + O2 the enzyme does not use NADH or NADPH as electron donor or H2O2 as electron acceptor Methanobrevibacter arboriphilus DSM Z 744 2 oxidized coenzyme F420 + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.3.22 ? x * 43000, SDS-PAGE Methanothermobacter marburgensis
1.5.3.22 ? x * 45000, SDS-PAGE Methanobrevibacter arboriphilus
1.5.3.22 ? x * 45300, calculated from sequence Methanothermobacter marburgensis
1.5.3.22 ? x * 46100, calculated from sequence Methanobrevibacter arboriphilus

Synonyms

EC Number Synonyms Comment Organism
1.5.3.22 FprA
-
 Methanothermobacter marburgensis
1.5.3.22 FprA
-
 Methanobrevibacter arboriphilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.3.22 37
-
 assay at Methanobrevibacter arboriphilus
1.5.3.22 65
-
 assay at Methanothermobacter marburgensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.3.22 7.5
-
-
 Methanothermobacter marburgensis
1.5.3.22 7.5
-
-
 Methanobrevibacter arboriphilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.3.22 6.5 8.5 30% of the maximal activity at pH 6.5 and 8.5 Methanobrevibacter arboriphilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.22 FMN the 45000 Da protein contains 1 FMN per mol Methanothermobacter marburgensis
1.5.3.22 FMN the 45000 Da protein contains 1 FMN per mol Methanobrevibacter arboriphilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.5.3.22 Methanobrevibacter arboriphilus calculated from sequence
-
 5.3

General Information

EC Number General Information Comment Organism
1.5.3.22 physiological function the enzyme is involved in O2 detoxification Methanothermobacter marburgensis
1.5.3.22 physiological function the enzyme is involved in O2 detoxification Methanobrevibacter arboriphilus