EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.40 | additional information | the enzyme is not inhibited by iodoacetamide (20 mM), chloromercuribenzoate (20 mM) or N-ethylmaleimide (7 mM) when preincubated for 30 min in the presence of these reagents. The enzyme is not inactivated by dioxygen | Archaeoglobus fulgidus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.40 | 0.01 | - |
oxidized coenzyme F420 | pH 5.5, 65°C | Archaeoglobus fulgidus | |
1.5.1.40 | 0.02 | - |
reduced coenzyme F420 | pH 8.0, 65°C | Archaeoglobus fulgidus | |
1.5.1.40 | 0.04 | - |
NADP+ | pH 8.0, 65°C | Archaeoglobus fulgidus | |
1.5.1.40 | 0.04 | - |
NADPH | pH 5.5, 65°C | Archaeoglobus fulgidus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.40 | 28000 | - |
2 * 28000, SDS-PAGE | Archaeoglobus fulgidus |
1.5.1.40 | 52000 | - |
sedimentation analysis | Archaeoglobus fulgidus |
1.5.1.40 | 57000 | - |
gel filtration | Archaeoglobus fulgidus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.40 | reduced coenzyme F420 + NADP+ | Archaeoglobus fulgidus | - |
oxidized coenzyme F420 + NADPH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.40 | Archaeoglobus fulgidus | O29370 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.40 | - |
Archaeoglobus fulgidus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.1.40 | 660 | - |
pH 8.0, 80°C | Archaeoglobus fulgidus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.40 | reduced coenzyme F420 + NADP+ | - |
Archaeoglobus fulgidus | oxidized coenzyme F420 + NADPH + H+ | - |
r | |
1.5.1.40 | reduced coenzyme F420 + NADP+ | the enzyme is Si face specific with respect to C5 of reduced coenzyme F420 and Si face specific with respect to C4 of NADP+. The enzyme is specific for both coenzyme F420 and NADP+/NADPH | Archaeoglobus fulgidus | oxidized coenzyme F420 + NADPH + H+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.40 | dimer | 2 * 28000, SDS-PAGE | Archaeoglobus fulgidus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.1.40 | F420-dependent NADP reductase | - |
Archaeoglobus fulgidus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.40 | 80 | - |
- |
Archaeoglobus fulgidus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.40 | 5.5 | - |
reduction of oxidized coenzyme F420 with NADPH | Archaeoglobus fulgidus |
1.5.1.40 | 8 | - |
reduction of NADP+ with reduced coenzyme F420 | Archaeoglobus fulgidus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.40 | 4.5 | 6.5 | pH 4.5: about 45% of maximal activity, pH 6.5: about 35% of maximal activity, reduction of oxidized coenzyme F420 with NADPH | Archaeoglobus fulgidus |
1.5.1.40 | 7 | 9 | pH 7.0: about 60% of maximal activity, pH 9.0: about 50% of maximal activity, reduction of NADP+ with reduced coenzyme F420 | Archaeoglobus fulgidus |