EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.16 | additional information | the enzyme is not influenced by 5 mM EDTA | Mycobacterium tuberculosis | |
1.2.1.16 | succinate semialdehyde | - |
Mycobacterium tuberculosis | |
1.2.1.79 | Succinic semialdehyde | partial substrate inhibition because velocity decreases to a non-zero value at saturating concentrations of Mg2+ and succinic semialdehyde | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.16 | 0.003 | - |
succinate semialdehyde | with NADP+ as cosubstrate, in the presence of 10 mM Mg2+, at pH 7.5 and 37°C | Mycobacterium tuberculosis | |
1.2.1.16 | 0.0092 | - |
NAD+ | in the presence of 10 mM Mg2+, at pH 7.5 and 37°C | Mycobacterium tuberculosis | |
1.2.1.16 | 0.0133 | - |
succinate semialdehyde | with NADP+ as cosubstrate, in the absence of Mg2+, at pH 7.5 and 37°C | Mycobacterium tuberculosis | |
1.2.1.16 | 0.0613 | - |
NADP+ | in the absence of Mg2+, at pH 7.5 and 37°C | Mycobacterium tuberculosis | |
1.2.1.79 | 0.003 | - |
Succinic semialdehyde | steady-state parameter, 200 microM succinic semialdehyde and 10 mM Mg2+, at pH 7.5 and 37°C | Mycobacterium tuberculosis | |
1.2.1.79 | 0.0092 | - |
NADP+ | steady-state parameter, 50 microM NADP+ and 10 mM Mg2+, at pH 7.5 and 37°C | Mycobacterium tuberculosis | |
1.2.1.79 | 0.0133 | - |
Succinic semialdehyde | steady-state parameter, 200 microM succinic semialdehyde, at pH 7.5 and 37°C | Mycobacterium tuberculosis | |
1.2.1.79 | 0.0613 | - |
NADP+ | steady-state parameter, 50 microM NADP+, at pH 7.5 and 37°C | Mycobacterium tuberculosis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.16 | Ca2+ | - |
Mycobacterium tuberculosis | |
1.2.1.16 | Co2+ | - |
Mycobacterium tuberculosis | |
1.2.1.16 | Mg2+ | 100% activity at 10 mM Mg2+. With Mg2+ there is a 4fold decrease in the Km value for succinate semialdehyde and a 6fold decrease in the Km value for NADP+ | Mycobacterium tuberculosis | |
1.2.1.16 | Mn2+ | - |
Mycobacterium tuberculosis | |
1.2.1.16 | additional information | not activated by Cd2+ and Zn2+ | Mycobacterium tuberculosis | |
1.2.1.16 | Ni2+ | - |
Mycobacterium tuberculosis | |
1.2.1.79 | Ca2+ | 2 to 3-fold activation at saturating concentration | Mycobacterium tuberculosis | |
1.2.1.79 | Co2+ | 2 to 3-fold activation at saturating concentration | Mycobacterium tuberculosis | |
1.2.1.79 | Mg2+ | 2 to 3-fold activation at saturating concentration | Mycobacterium tuberculosis | |
1.2.1.79 | Mn2+ | 2 to 3-fold activation at saturating concentration | Mycobacterium tuberculosis | |
1.2.1.79 | additional information | no activation with Cd2+ or Zn2+ | Mycobacterium tuberculosis | |
1.2.1.79 | Ni2+ | 2 to 3-fold activation at saturating concentration | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.16 | additional information | Mycobacterium tuberculosis | other aldehydes, such as formaldehyde, acetaldehyde and glutaraldehyde, are very poor substrates | ? | - |
? | |
1.2.1.16 | succinate semialdehyde + NAD+ + H2O | Mycobacterium tuberculosis | - |
succinate + NADH + H+ | - |
? | |
1.2.1.16 | succinate semialdehyde + NADP+ + H2O | Mycobacterium tuberculosis | - |
succinate + NADPH + H+ | - |
? | |
1.2.1.79 | succinic semialdehyde + NADP+ + H2O | Mycobacterium tuberculosis | chemical mechanism based on functional data and structural information proposed, 1H-NMR to probe the stereospecificity of GabD1 show a transfer of the deuteride to the pro-R position of NADP+ indicating GabD1 has A-type stereospecificity | succinate + NADPH + 2 H+ | - |
ir | |
1.2.1.79 | succinic semialdehyde + NADP+ + H2O | Mycobacterium tuberculosis H37Rv | chemical mechanism based on functional data and structural information proposed, 1H-NMR to probe the stereospecificity of GabD1 show a transfer of the deuteride to the pro-R position of NADP+ indicating GabD1 has A-type stereospecificity | succinate + NADPH + 2 H+ | - |
ir |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.16 | Mycobacterium tuberculosis | - |
- |
- |
1.2.1.79 | Mycobacterium tuberculosis | P9WNX9 | - |
- |
1.2.1.79 | Mycobacterium tuberculosis H37Rv | P9WNX9 | - |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.2.1.16 | there is no significant loss of catalytic activity, after up to 40 min of incubation of isoform GabD1 with 1 mM H2O2 at pH 7.4 | Mycobacterium tuberculosis |
1.2.1.79 | GabD1 is naturally resistant to oxidation by H2O2 | Mycobacterium tuberculosis |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.16 | Ni-NTA resin column chromatography | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.16 | additional information | other aldehydes, such as formaldehyde, acetaldehyde and glutaraldehyde, are very poor substrates | Mycobacterium tuberculosis | ? | - |
? | |
1.2.1.16 | succinate semialdehyde + NAD+ + H2O | - |
Mycobacterium tuberculosis | succinate + NADH + H+ | - |
? | |
1.2.1.16 | succinate semialdehyde + NADP+ + H2O | - |
Mycobacterium tuberculosis | succinate + NADPH + H+ | - |
? | |
1.2.1.79 | additional information | other aldehydes, such as formaldehyde, acetaldehyde and glutaraldehyde, are very poor substrates showing a narrow substrate specificity of GabD1 | Mycobacterium tuberculosis | ? | - |
? | |
1.2.1.79 | additional information | other aldehydes, such as formaldehyde, acetaldehyde and glutaraldehyde, are very poor substrates showing a narrow substrate specificity of GabD1 | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
1.2.1.79 | succinic semialdehyde + NADP+ + H2O | chemical mechanism based on functional data and structural information proposed, 1H-NMR to probe the stereospecificity of GabD1 show a transfer of the deuteride to the pro-R position of NADP+ indicating GabD1 has A-type stereospecificity | Mycobacterium tuberculosis | succinate + NADPH + 2 H+ | - |
ir | |
1.2.1.79 | succinic semialdehyde + NADP+ + H2O | chemical mechanism based on functional data and structural information proposed, 1H-NMR to probe the stereospecificity of GabD1 show a transfer of the deuteride to the pro-R position of NADP+ indicating GabD1 has A-type stereospecificity | Mycobacterium tuberculosis H37Rv | succinate + NADPH + 2 H+ | - |
ir |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.16 | GabD1 | isoform | Mycobacterium tuberculosis |
1.2.1.16 | succinic semialdehyde dehydrogenase | - |
Mycobacterium tuberculosis |
1.2.1.79 | GabD1 | - |
Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.16 | 1.3 | - |
NADP+ | in the absence of Mg2+, at pH 7.5 and 37°C | Mycobacterium tuberculosis | |
1.2.1.16 | 1.9 | - |
succinate semialdehyde | with NADP+ as cosubstrate, in the absence of Mg2+, at pH 7.5 and 37°C | Mycobacterium tuberculosis | |
1.2.1.16 | 2.7 | - |
NAD+ | in the presence of 10 mM Mg2+, at pH 7.5 and 37°C | Mycobacterium tuberculosis | |
1.2.1.16 | 4.7 | - |
succinate semialdehyde | with NADP+ as cosubstrate, in the presence of 10 mM Mg2+, at pH 7.5 and 37°C | Mycobacterium tuberculosis | |
1.2.1.79 | 1.3 | - |
NADP+ | steady-state parameter, 50 microM NADP+, at pH 7.5 and 37°C | Mycobacterium tuberculosis | |
1.2.1.79 | 1.9 | - |
Succinic semialdehyde | steady-state parameter, 200 microM succinic semialdehyde, at pH 7.5 and 37°C | Mycobacterium tuberculosis | |
1.2.1.79 | 2.7 | - |
NADP+ | steady-state parameter, 50 microM NADP+ and 10 mM Mg2+, at pH 7.5 and 37°C | Mycobacterium tuberculosis | |
1.2.1.79 | 4.7 | - |
Succinic semialdehyde | steady-state parameter, 200 microM succinic semialdehyde and 10 mM Mg2+, at pH 7.5 and 37°C | Mycobacterium tuberculosis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.16 | 5.5 | 10 | the enzyme cannot be assayed below pH 5.5, due to precipitation and loss of activity | Mycobacterium tuberculosis |
1.2.1.79 | 5.5 | 10 | GabD1 could not be assayed below pH 5.5, due to precipitation and loss of activity | Mycobacterium tuberculosis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.16 | NAD+ | long incubations lead to a modest utilization of NAD+ as substrate | Mycobacterium tuberculosis | |
1.2.1.16 | NADP+ | although long incubations lead to a modest utilization of NAD+ as substrate, NADP+ is the preferred substrate | Mycobacterium tuberculosis | |
1.2.1.79 | NAD+ | long incubations lead to modest utilization | Mycobacterium tuberculosis | |
1.2.1.79 | NADP+ | preferred substrate | Mycobacterium tuberculosis |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.16 | 0.0628 | - |
succinate semialdehyde | in the absence of Mg2+, at pH 7.5 and 37°C | Mycobacterium tuberculosis | |
1.2.1.79 | 0.0628 | - |
Succinic semialdehyde | partial substrate inhibition, pH 7.5 and 37°C | Mycobacterium tuberculosis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.2.1.79 | metabolism | enzyme catalyzes the last step of the gamma-aminobutyrate degradation | Mycobacterium tuberculosis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.16 | 140 | - |
succinate semialdehyde | with NADP+ as cosubstrate, in the absence of Mg2+, at pH 7.5 and 37°C | Mycobacterium tuberculosis | |
1.2.1.16 | 210 | - |
NADP+ | in the absence of Mg2+, at pH 7.5 and 37°C | Mycobacterium tuberculosis | |
1.2.1.16 | 290 | - |
NAD+ | in the presence of 10 mM Mg2+, at pH 7.5 and 37°C | Mycobacterium tuberculosis | |
1.2.1.16 | 1600 | - |
succinate semialdehyde | with NADP+ as cosubstrate, in the presence of 10 mM Mg2+, at pH 7.5 and 37°C | Mycobacterium tuberculosis | |
1.2.1.79 | 21 | - |
NADP+ | steady-state parameter, 50 microM NADP+, at pH 7.5 and 37°C | Mycobacterium tuberculosis | |
1.2.1.79 | 140 | - |
Succinic semialdehyde | steady-state parameter, 200 microM succinic semialdehyde, at pH 7.5 and 37°C | Mycobacterium tuberculosis | |
1.2.1.79 | 290 | - |
NADP+ | steady-state parameter, 50 microM NADP+ and 10 mM Mg2+, at pH 7.5 and 37°C | Mycobacterium tuberculosis | |
1.2.1.79 | 1600 | - |
Succinic semialdehyde | steady-state parameter, 200 microM succinic semialdehyde and 10 mM Mg2+, at pH 7.5 and 37°C | Mycobacterium tuberculosis |