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Literature summary extracted from

  • Przybysz, A.; Volmer, A.A.; Westphal, A.H.; van Berkel, W.J.
    Bifunctional immobilization of a hyperthermostable endo-beta-1,3-glucanase (2014), Appl. Microbiol. Biotechnol., 98, 1155-1163.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.39 expression in Escherichia coli Pyrococcus furiosus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.39 S296C the immobilized S296C variant shows extreme pH stability and can be repeatedly used at 60°C without significant activity loss of activity Pyrococcus furiosus

General Stability

EC Number General Stability Organism
3.2.1.39 operational stability of the immobilized S296C variant is optimal with milled beads suggesting applicability of the immobilized enzyme on the smallest particles for continuous substrate conversion under industrially relevant conditions. S296C bound to the large beads shows a somewhat lower operational stability Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.39 Laminarin
-
 Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.39 0.2
-
 Laminarin pH 6.5, 90°C, soluble S296C variant Pyrococcus furiosus
3.2.1.39 0.77
-
 Laminarin pH 6.5, 90°C, S296C variant immobilized on beads of 0.02-0.04 mM size Pyrococcus furiosus
3.2.1.39 1
-
 Laminarin pH 6.5, 90°C, S296C variant immobilized on beads of 0.063-0.09 mM size Pyrococcus furiosus
3.2.1.39 2.9
-
 Laminarin pH 6.5, 90°C, S296C variant immobilized on beads of 0.15-0.3 mM size Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Pyrococcus furiosus O73951
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.39
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 laminarin + H2O
-
 Pyrococcus furiosus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.39 90
-
 assay at Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.39 60
-
 bifunctional immobilized S296C enzyme shows high operational stability and pH stability at 60°C Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.39 6.5
-
 assay at Pyrococcus furiosus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.39 4 6 60°C, 48 h, incubation does not significantly reduce the activity of the immobilized S296C variant Pyrococcus furiosus
3.2.1.39 10
-
 60°C, 48 h, incubation does not significantly reduce the activity of the immobilized S296C variant Pyrococcus furiosus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.39 3.5
-
 Laminarin pH 6.5, 90°C, S296C variant immobilized on beads of 0.02-0.04 mM size Pyrococcus furiosus
3.2.1.39 6.5
-
 Laminarin pH 6.5, 90°C, soluble S296C variant Pyrococcus furiosus
3.2.1.39 22.7
-
 Laminarin pH 6.5, 90°C, S296C variant immobilized on beads of 0.063-0.09 mM size Pyrococcus furiosus