EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.5.5 | genes adhA and adhB, encoding subunits I and II, DNA and amino acid sequence determination and analysis,phylogenetic tree | Frateuria aurantia |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.5.11 | acetic acid | about 20% residual activity at 8% (v/v) acetic acid | Frateuria aurantia |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.5.5 | membrane | bound | Frateuria aurantia | 16020 | - |
1.1.5.11 | membrane | - |
Frateuria aurantia | 16020 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.5.5 | 45000 | - |
1 * 72000 + 1 * 45000, SDS-PAGE | Frateuria aurantia |
1.1.5.5 | 72000 | - |
1 * 72000 + 1 * 45000, SDS-PAGE | Frateuria aurantia |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.5.5 | ethanol + ubiquinone | Frateuria aurantia | - |
acetaldehyde + ubiquinol | - |
? | |
1.1.5.5 | ethanol + ubiquinone | Frateuria aurantia LMG 1558 | - |
acetaldehyde + ubiquinol | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.5.5 | Frateuria aurantia | CCU55317 | genes adhA and adhB encoding subunits I and II | - |
1.1.5.5 | Frateuria aurantia LMG 1558 | CCU55317 | genes adhA and adhB encoding subunits I and II | - |
1.1.5.11 | Frateuria aurantia | S6D3Q2 | - |
- |
1.1.5.11 | Frateuria aurantia LMG 1558T | S6D3Q2 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.5.5 | native enzyme from membranes by anion exchange and cation exchange chromatography, followed by hydroxyapatite chromatography, to near homogeneity | Frateuria aurantia |
1.1.5.11 | DEAE Toyopearl column chromatography, CM Toyopearl column chromatography, and hydroxyapatite column chromatography | Frateuria aurantia |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.5.5 | 3 | - |
enzyme in cell membranes, pH 4.5, 20°C | Frateuria aurantia |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.5.5 | ethanol + ubiquinone | - |
Frateuria aurantia | acetaldehyde + ubiquinol | - |
? | |
1.1.5.5 | ethanol + ubiquinone | - |
Frateuria aurantia LMG 1558 | acetaldehyde + ubiquinol | - |
? | |
1.1.5.5 | additional information | substrate specificity, overview | Frateuria aurantia | ? | - |
? | |
1.1.5.5 | additional information | substrate specificity, overview | Frateuria aurantia LMG 1558 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.5.5 | heterodimer | 1 * 72000 + 1 * 45000, SDS-PAGE | Frateuria aurantia |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.5.5 | PQQ-ADH | - |
Frateuria aurantia |
1.1.5.5 | PQQ-dependent alcohol dehydrogenase | - |
Frateuria aurantia |
1.1.5.5 | pyrroloquinoline quinone-dependent alcohol dehydrogenase | - |
Frateuria aurantia |
1.1.5.11 | 1-butanol dehydrogenase | - |
Frateuria aurantia |
1.1.5.11 | AdhA | - |
Frateuria aurantia |
1.1.5.11 | PQQ-ADH | - |
Frateuria aurantia |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.5.5 | 20 | - |
- |
Frateuria aurantia |
1.1.5.11 | 20 | - |
- |
Frateuria aurantia |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.5.5 | 10 | 50 | activity range, profile overview. No activity above 50°C, maximum activity at 20°C | Frateuria aurantia |
1.1.5.11 | 10 | 40 | more than 60% activity between 10 and 40°C, no activity at 50°C | Frateuria aurantia |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.5.5 | 4.5 | - |
- |
Frateuria aurantia |
1.1.5.11 | 5 | - |
- |
Frateuria aurantia |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.5.5 | 3 | 6 | strong decrease of activity at pH levels below pH 4 and above pH 5.5, and no activity at pH 2.0 and pH 7.0, activity range, profile overview | Frateuria aurantia |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.5.5 | pyrroloquinoline quinone | dependent on | Frateuria aurantia | |
1.1.5.11 | heme c | - |
Frateuria aurantia | |
1.1.5.11 | pyrroloquinoline quinone | - |
Frateuria aurantia |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.5.5 | evolution | high similarity between genes encoding subunits I and II of PQQ-ADH | Frateuria aurantia |