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Literature summary extracted from

  • Kawakami, R.; Noguchi, C.; Higashi, M.; Sakuraba, H.; Ohshima, T.
    Comparative analysis of the catalytic components in the archaeal dye-linked L-proline dehydrogenase complexes (2013), Appl. Microbiol. Biotechnol., 97, 3419-3427.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.99.B2 expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL and BL21-Gold(DE3)pLysS cells Pyrococcus furiosus
1.5.99.B2 expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL and BL21-Gold(DE3)pLysS cells Pyrococcus horikoshii
1.5.99.B2 expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL and BL21-Gold(DE3)pLysS cells Thermococcus kodakarensis
1.5.99.B2 expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL and BL21-Gold(DE3)pLysS cells Thermococcus profundus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.99.B2 L-pyroglutamate
-
 Pyrococcus furiosus
1.5.99.B2 L-pyroglutamate
-
 Pyrococcus horikoshii
1.5.99.B2 L-pyroglutamate
-
 Thermococcus kodakarensis
1.5.99.B2 L-pyroglutamate
-
 Thermococcus profundus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.99.B2 0.174
-
 L-proline PF1798 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Pyrococcus furiosus
1.5.99.B2 0.334
-
 L-proline PH1751 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Pyrococcus horikoshii
1.5.99.B2 0.689
-
 L-proline PF1246 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Pyrococcus furiosus
1.5.99.B2 1.46
-
 L-proline TK0117 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Thermococcus kodakarensis
1.5.99.B2 2.38
-
 L-proline TK0122 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Thermococcus kodakarensis
1.5.99.B2 2.46
-
 L-proline TPpdhB (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Thermococcus profundus
1.5.99.B2 2.5 5 L-proline PF1364 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Pyrococcus horikoshii
1.5.99.B2 3.5
-
 L-proline TPpdhB2 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Thermococcus profundus

Organism

EC Number Organism UniProt Comment Textmining
1.5.99.B2 Pyrococcus furiosus
-
-
-
1.5.99.B2 Pyrococcus horikoshii
-
-
-
1.5.99.B2 Pyrococcus horikoshii OT-3
-
-
-
1.5.99.B2 Thermococcus kodakarensis
-
-
-
1.5.99.B2 Thermococcus kodakarensis KOD1 JCM 12380
-
-
-
1.5.99.B2 Thermococcus profundus Q76M73
-
-
1.5.99.B2 Thermococcus profundus DSM 9503 Q76M73
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.99.B2 Ni-chelating column chromatography Pyrococcus furiosus
1.5.99.B2 Ni-chelating column chromatography Pyrococcus horikoshii
1.5.99.B2 Ni-chelating column chromatography Thermococcus kodakarensis
1.5.99.B2 Ni-chelating column chromatography Thermococcus profundus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.99.B2 L-proline + 2,6-dichlorophenolindophenol + H2O the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor Pyrococcus furiosus DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.B2 L-proline + 2,6-dichlorophenolindophenol + H2O the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor Pyrococcus horikoshii DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.B2 L-proline + 2,6-dichlorophenolindophenol + H2O the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor Thermococcus kodakarensis DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.B2 L-proline + 2,6-dichlorophenolindophenol + H2O the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor Thermococcus profundus DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.B2 L-proline + 2,6-dichlorophenolindophenol + H2O the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor Thermococcus profundus DSM 9503 DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.B2 L-proline + 2,6-dichlorophenolindophenol + H2O the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor Pyrococcus horikoshii OT-3 DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.B2 L-proline + 2,6-dichlorophenolindophenol + H2O the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor Thermococcus kodakarensis KOD1 JCM 12380 DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.5.99.B2 L-proline + 4-iodonitrotetrazolium violet + H2O
-
 Pyrococcus furiosus DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
 ?
1.5.99.B2 L-proline + 4-iodonitrotetrazolium violet + H2O
-
 Pyrococcus horikoshii DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
 ?
1.5.99.B2 L-proline + 4-iodonitrotetrazolium violet + H2O
-
 Thermococcus kodakarensis DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
 ?
1.5.99.B2 L-proline + 4-iodonitrotetrazolium violet + H2O
-
 Thermococcus profundus DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
 ?
1.5.99.B2 L-proline + 4-iodonitrotetrazolium violet + H2O
-
 Thermococcus profundus DSM 9503 DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
 ?
1.5.99.B2 L-proline + 4-iodonitrotetrazolium violet + H2O
-
 Pyrococcus horikoshii OT-3 DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
 ?
1.5.99.B2 L-proline + 4-iodonitrotetrazolium violet + H2O
-
 Thermococcus kodakarensis KOD1 JCM 12380 DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
 ?
1.5.99.B2 L-proline + ferricyanide the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor Pyrococcus furiosus DELTA1-pyrroline-5-carboxylate + ferrocyanide
-
 ?
1.5.99.B2 L-proline + ferricyanide the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor Pyrococcus horikoshii DELTA1-pyrroline-5-carboxylate + ferrocyanide
-
 ?
1.5.99.B2 L-proline + ferricyanide the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor Thermococcus kodakarensis DELTA1-pyrroline-5-carboxylate + ferrocyanide
-
 ?
1.5.99.B2 L-proline + ferricyanide the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor Thermococcus profundus DELTA1-pyrroline-5-carboxylate + ferrocyanide
-
 ?
1.5.99.B2 L-proline + ferricyanide the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor Thermococcus profundus DSM 9503 DELTA1-pyrroline-5-carboxylate + ferrocyanide
-
 ?
1.5.99.B2 L-proline + ferricyanide the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor Pyrococcus horikoshii OT-3 DELTA1-pyrroline-5-carboxylate + ferrocyanide
-
 ?
1.5.99.B2 L-proline + ferricyanide the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor Thermococcus kodakarensis KOD1 JCM 12380 DELTA1-pyrroline-5-carboxylate + ferrocyanide
-
 ?
1.5.99.B2 additional information D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates Pyrococcus furiosus ?
-
 ?
1.5.99.B2 additional information D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates Pyrococcus horikoshii ?
-
 ?
1.5.99.B2 additional information D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates Thermococcus kodakarensis ?
-
 ?
1.5.99.B2 additional information D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates Thermococcus profundus ?
-
 ?
1.5.99.B2 additional information D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates Thermococcus profundus DSM 9503 ?
-
 ?
1.5.99.B2 additional information D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates Pyrococcus horikoshii OT-3 ?
-
 ?
1.5.99.B2 additional information D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates Thermococcus kodakarensis KOD1 JCM 12380 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.99.B2 ? x * 44000-45000, SDS-PAGE Pyrococcus furiosus
1.5.99.B2 ? x * 44000-45000, SDS-PAGE Pyrococcus horikoshii
1.5.99.B2 ? x * 44000-45000, SDS-PAGE Thermococcus kodakarensis
1.5.99.B2 ? x * 44000-45000, SDS-PAGE Thermococcus profundus

Synonyms

EC Number Synonyms Comment Organism
1.5.99.B2 L-proline dehydrogenase
-
 Pyrococcus furiosus
1.5.99.B2 L-proline dehydrogenase
-
 Pyrococcus horikoshii
1.5.99.B2 L-proline dehydrogenase
-
 Thermococcus kodakarensis
1.5.99.B2 L-proline dehydrogenase
-
 Thermococcus profundus
1.5.99.B2 PDH
-
 Pyrococcus furiosus
1.5.99.B2 PDH
-
 Pyrococcus horikoshii
1.5.99.B2 PDH
-
 Thermococcus kodakarensis
1.5.99.B2 PDH
-
 Thermococcus profundus
1.5.99.B2 PF1246 alpha4beta4-type beta subunit of L-proline dehydrogenase Pyrococcus furiosus
1.5.99.B2 PF1798 alphabetagammadelta-type beta subunit of L-proline dehydrogenase Pyrococcus furiosus
1.5.99.B2 PH1364 alpha4beta4-type beta subunit of L-proline dehydrogenase Pyrococcus horikoshii
1.5.99.B2 PH1751 alphabetagammadelta-type beta subunit of L-proline dehydrogenase Pyrococcus horikoshii
1.5.99.B2 PRODH
-
 Pyrococcus furiosus
1.5.99.B2 PRODH
-
 Pyrococcus horikoshii
1.5.99.B2 PRODH
-
 Thermococcus kodakarensis
1.5.99.B2 PRODH
-
 Thermococcus profundus
1.5.99.B2 TK0117 alpha4beta4-type beta subunit of L-proline dehydrogenase Thermococcus kodakarensis
1.5.99.B2 TK0122 alphabetagammadelta-type beta subunit of L-proline dehydrogenase Thermococcus kodakarensis
1.5.99.B2 TPpdhB alphabetagammadelta-type beta subunit of L-proline dehydrogenase Thermococcus profundus
1.5.99.B2 TPpdhB2 alpha4beta4-type beta subunit of L-proline dehydrogenase Thermococcus profundus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.99.B2 65
-
 alphabetagammadelta-type beta subunit of L-proline dehydrogenase Pyrococcus furiosus
1.5.99.B2 65
-
 alphabetagammadelta-type beta subunit of L-proline dehydrogenase Pyrococcus horikoshii
1.5.99.B2 65
-
 alphabetagammadelta-type beta subunit of L-proline dehydrogenase Thermococcus kodakarensis
1.5.99.B2 65
-
 alphabetagammadelta-type beta subunit of L-proline dehydrogenase Thermococcus profundus
1.5.99.B2 80
-
 alpha4beta4-type beta subunit of L-proline dehydrogenase Pyrococcus furiosus
1.5.99.B2 80
-
 alpha4beta4-type beta subunit of L-proline dehydrogenase Pyrococcus horikoshii
1.5.99.B2 80
-
 alpha4beta4-type beta subunit of L-proline dehydrogenase Thermococcus kodakarensis
1.5.99.B2 80
-
 alpha4beta4-type beta subunit of L-proline dehydrogenase Thermococcus profundus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.99.B2 70
-
 the alpha4beta4-type beta subunit of L-proline dehydrogenase retains more than 80% of activity after heating at 70°C for 20 min, while the alphabetagammadelta-type beta subunit of L-proline dehydrogenase nearly loses all of its activity under the same conditions Pyrococcus horikoshii
1.5.99.B2 70
-
 the alpha4beta4-type beta subunit of L-proline dehydrogenase retains more than 80% of activity after heating at 70°C for 20 min, while the alphabetagammadelta-type beta subunit of L-proline dehydrogenase retains 100% activity under the same conditions Pyrococcus furiosus
1.5.99.B2 70
-
 the alpha4beta4-type beta subunit of L-proline dehydrogenase retains more than 80% of activity after heating at 70°C for 20 min, while the alphabetagammadelta-type beta subunit of L-proline dehydrogenase shows 68% activity under the same conditions Thermococcus profundus
1.5.99.B2 70
-
 the alpha4beta4-type beta subunit of L-proline dehydrogenase retains more than 80% of activity after heating at 70°C for 20 min, while the alphabetagammadelta-type beta subunit of L-proline dehydrogenase shows 76% activity under the same conditions Thermococcus kodakarensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.99.B2 1.96
-
 L-proline PH1751 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Pyrococcus horikoshii
1.5.99.B2 3.49
-
 L-proline PF1798 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Pyrococcus furiosus
1.5.99.B2 3.91
-
 L-proline PF1246 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Pyrococcus furiosus
1.5.99.B2 6.22
-
 L-proline TPpdhB2 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Thermococcus profundus
1.5.99.B2 11
-
 L-proline PF1364 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Pyrococcus horikoshii
1.5.99.B2 11.6
-
 L-proline TK0117 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Thermococcus kodakarensis
1.5.99.B2 27.6
-
 L-proline TK0122 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Thermococcus kodakarensis
1.5.99.B2 27.9
-
 L-proline TPpdhB (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Thermococcus profundus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.99.B2 FAD holoprotein contains one FAD per molecule Pyrococcus furiosus
1.5.99.B2 FAD holoprotein contains one FAD per molecule Pyrococcus horikoshii
1.5.99.B2 FAD holoprotein contains one FAD per molecule Thermococcus kodakarensis
1.5.99.B2 FAD holoprotein contains one FAD per molecule Thermococcus profundus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.99.B2 24
-
 L-pyroglutamate PH1751 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Pyrococcus horikoshii
1.5.99.B2 49.4
-
 L-pyroglutamate TK0117 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Thermococcus kodakarensis
1.5.99.B2 55.5
-
 L-pyroglutamate PF1364 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Pyrococcus horikoshii
1.5.99.B2 56.1
-
 L-pyroglutamate TPpdhB (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Thermococcus profundus
1.5.99.B2 68.3
-
 L-pyroglutamate PF1246 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Pyrococcus furiosus
1.5.99.B2 122
-
 L-pyroglutamate TPpdhB2 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Thermococcus profundus
1.5.99.B2 129
-
 L-pyroglutamate PF1798 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Pyrococcus furiosus
1.5.99.B2 167
-
 L-pyroglutamate TK0122 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C Thermococcus kodakarensis