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Literature summary extracted from

  • Pisanelli, I.; Wuehrer, P.; Reyes-Dominguez, Y.; Spadiut, O.; Haltrich, D.; Peterbauer, C.
    Heterologous expression and biochemical characterization of novel pyranose 2-oxidases from the ascomycetes Aspergillus nidulans and Aspergillus oryzae (2012), Appl. Microbiol. Biotechnol., 93, 1157-1166.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.10 DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Aspergillus nidulans
1.1.3.10 DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Aspergillus oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.10 0.11
-
 2-methyl-1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 0.15
-
 1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 0.32
-
 1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus oryzae
1.1.3.10 0.39
-
 2,6-dichloroindophenol pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 0.4
-
 2,6-dimethyl-1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 0.42
-
 2-methoxy-1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 0.48
-
 2-chloro-1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 0.67
-
 ferricenium hexafluorophosphate pH 8.0, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 1.12
-
 ferricenium hexafluorophosphate pH 8.0, 30°C, recombinant enzyme Aspergillus oryzae
1.1.3.10 1.77
-
 D-glucose pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 2.86
-
 D-glucose pH 6.5, 30°C, recombinant enzyme Aspergillus oryzae
1.1.3.10 9.42
-
 tetrafluoro-1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 13
-
 D-galactose pH 6.5, 30°C, recombinant enzyme Aspergillus oryzae
1.1.3.10 18.1
-
 D-xylose pH 6.5, 30°C, recombinant enzyme Aspergillus oryzae
1.1.3.10 32.6
-
 L-sorbose pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 66.9
-
 D-xylose pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 176
-
 L-sorbose pH 6.5, 30°C, recombinant enzyme Aspergillus oryzae
1.1.3.10 192
-
 D-galactose pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 2558
-
 D-fructose pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.10 65000
-
 x * 65000, SDS-PAGE Aspergillus nidulans
1.1.3.10 65000
-
 x * 65000, SDS-PAGE Aspergillus oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.10 D-glucose + O2 Aspergillus nidulans
-
 2-dehydro-D-glucose + H2O2
-
 ?
1.1.3.10 D-glucose + O2 Aspergillus oryzae
-
 2-dehydro-D-glucose + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.10 Aspergillus nidulans
-
-
-
1.1.3.10 Aspergillus oryzae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.10 recombinant His-tagged enzyme 232fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Aspergillus oryzae
1.1.3.10 recombinant His-tagged enzyme 6.4fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Aspergillus nidulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.10 0.58
-
 purified recombinant His-tagged enzyme, pH 6.5, 30°C Aspergillus oryzae
1.1.3.10 1.16
-
 purified recombinant His-tagged enzyme, pH 6.5, 30°C Aspergillus nidulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.10 D-fructose + O2
-
 Aspergillus nidulans 2-dehydro-D-fructose + H2O2
-
 ?
1.1.3.10 D-galactose + O2
-
 Aspergillus nidulans 2-dehydro-D-galactose + H2O2
-
 ?
1.1.3.10 D-galactose + O2
-
 Aspergillus oryzae 2-dehydro-D-galactose + H2O2
-
 ?
1.1.3.10 D-glucose + 1,4-benzoquinone
-
 Aspergillus nidulans ?
-
 ?
1.1.3.10 D-glucose + 1,4-benzoquinone
-
 Aspergillus oryzae ?
-
 ?
1.1.3.10 D-glucose + 2,6-dichloroindophenol
-
 Aspergillus nidulans ?
-
 ?
1.1.3.10 D-glucose + 2,6-dimethyl-1,4-benzoquinone
-
 Aspergillus nidulans ?
-
 ?
1.1.3.10 D-glucose + 2-chloro-1,4-benzoquinone
-
 Aspergillus nidulans ?
-
 ?
1.1.3.10 D-glucose + 2-methoxy-1,4-benzoquinone
-
 Aspergillus nidulans ?
-
 ?
1.1.3.10 D-glucose + 2-methyl-1,4-benzoquinone
-
 Aspergillus nidulans ?
-
 ?
1.1.3.10 D-glucose + ferricenium hexafluorophosphate
-
 Aspergillus nidulans ?
-
 ?
1.1.3.10 D-glucose + ferricenium hexafluorophosphate
-
 Aspergillus oryzae ?
-
 ?
1.1.3.10 D-glucose + O2
-
 Aspergillus nidulans 2-dehydro-D-glucose + H2O2
-
 ?
1.1.3.10 D-glucose + O2
-
 Aspergillus oryzae 2-dehydro-D-glucose + H2O2
-
 ?
1.1.3.10 D-glucose + tetrafluoro-1,4-benzoquinone
-
 Aspergillus nidulans ?
-
 ?
1.1.3.10 D-xylose + O2
-
 Aspergillus nidulans 2-dehydro-D-xylose + H2O2
-
 ?
1.1.3.10 D-xylose + O2
-
 Aspergillus oryzae 2-dehydro-D-xylose + H2O2
-
 ?
1.1.3.10 L-sorbose + O2
-
 Aspergillus nidulans 5-dehydro-D-fructose + H2O2
-
 ?
1.1.3.10 L-sorbose + O2
-
 Aspergillus oryzae 5-dehydro-D-fructose + H2O2
-
 ?
1.1.3.10 additional information no activity with D-fructose, mannose and lactose as substrates. Peroxidase-coupled assay using [2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid)] as the chromogen Aspergillus oryzae ?
-
 ?
1.1.3.10 additional information no activity with mannose and lactose as substrates. The enzyme catalyzes the two-electron reduction of 1,4-benzoquinone, several substituted benzoquinones and 2,6-dichloroindophenol. Some of these quinone electron acceptors are better substrates for pyranose oxidase than oxygen. Peroxidase-coupled assay using [2,2'-azinobis(3-ethylbenzthiazolinesulfonic acid)] as the chromogen Aspergillus nidulans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.10 ? x * 65000, SDS-PAGE Aspergillus nidulans
1.1.3.10 ? x * 65000, SDS-PAGE Aspergillus oryzae

Synonyms

EC Number Synonyms Comment Organism
1.1.3.10 glucose 2-oxidase
-
 Aspergillus nidulans
1.1.3.10 glucose 2-oxidase
-
 Aspergillus oryzae
1.1.3.10 POX
-
 Aspergillus nidulans
1.1.3.10 POX
-
 Aspergillus oryzae
1.1.3.10 pyranose 2-Oxidase
-
 Aspergillus nidulans
1.1.3.10 pyranose 2-Oxidase
-
 Aspergillus oryzae
1.1.3.10 pyranose/oxygen 2-oxidoreductase
-
 Aspergillus nidulans
1.1.3.10 pyranose/oxygen 2-oxidoreductase
-
 Aspergillus oryzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.10 30
-
 assay at Aspergillus nidulans
1.1.3.10 30
-
 assay at Aspergillus oryzae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.10 0.02
-
 D-galactose pH 6.5, 30°C, recombinant enzyme Aspergillus oryzae
1.1.3.10 0.27
-
 D-xylose pH 6.5, 30°C, recombinant enzyme Aspergillus oryzae
1.1.3.10 0.4
-
 2,6-dimethyl-1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 1.39
-
 D-xylose pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 1.48
-
 D-glucose pH 6.5, 30°C, recombinant enzyme Aspergillus oryzae
1.1.3.10 1.59
-
 L-sorbose pH 6.5, 30°C, recombinant enzyme Aspergillus oryzae
1.1.3.10 1.7
-
 ferricenium hexafluorophosphate pH 8.0, 30°C, recombinant enzyme Aspergillus oryzae
1.1.3.10 1.95
-
 D-galactose pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 3.04
-
 1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus oryzae
1.1.3.10 5.55
-
 D-fructose pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 9.66
-
 L-sorbose pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 10.1
-
 2-methyl-1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 13.1
-
 2,6-dichloroindophenol pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 16.3
-
 2-chloro-1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 35.44
-
 D-glucose pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 47.2
-
 2-methoxy-1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 129
-
 tetrafluoro-1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 158
-
 ferricenium hexafluorophosphate pH 8.0, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 186
-
 1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.10 6.5
-
 assay at Aspergillus nidulans
1.1.3.10 6.5
-
 assay at Aspergillus oryzae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.10 flavin flavoprotein Aspergillus nidulans
1.1.3.10 flavin flavoprotein Aspergillus oryzae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.3.10 0.002
-
 D-galactose pH 6.5, 30°C, recombinant enzyme Aspergillus oryzae
1.1.3.10 0.009
-
 L-sorbose pH 6.5, 30°C, recombinant enzyme Aspergillus oryzae
1.1.3.10 0.015
-
 D-xylose pH 6.5, 30°C, recombinant enzyme Aspergillus oryzae
1.1.3.10 0.02
-
 D-xylose pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 0.1
-
 D-galactose pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 0.1
-
 2,6-dimethyl-1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 0.3
-
 L-sorbose pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 0.52
-
 D-glucose pH 6.5, 30°C, recombinant enzyme Aspergillus oryzae
1.1.3.10 1.5
-
 ferricenium hexafluorophosphate pH 8.0, 30°C, recombinant enzyme Aspergillus oryzae
1.1.3.10 9.4
-
 1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus oryzae
1.1.3.10 13.7
-
 tetrafluoro-1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 20.02
-
 D-glucose pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 22
-
 D-fructose pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 33.6
-
 2,6-dichloroindophenol pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 34
-
 2-chloro-1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 91.8
-
 2-methyl-1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 112
-
 2-methoxy-1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 235
-
 ferricenium hexafluorophosphate pH 8.0, 30°C, recombinant enzyme Aspergillus nidulans
1.1.3.10 1240
-
 1,4-benzoquinone pH 6.5, 30°C, recombinant enzyme Aspergillus nidulans