Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Faccio, G.; Kruus, K.; Buchert, J.; Saloheimo, M.
    Production and characterisation of AoSOX2 from Aspergillus oryzae, a novel flavin-dependent sulfhydryl oxidase with good pH and temperature stability (2011), Appl. Microbiol. Biotechnol., 90, 941-949.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.3.2 expression in Trichoderma reesei Aspergillus oryzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.3.2 MgSO4 1 mM, 55% residual activity Aspergillus oryzae
1.8.3.2 Na2SO4 1 mM, 66% residual activity Aspergillus oryzae
1.8.3.2 sodium dodecylsulfate 1 mM, 57% residual activity Aspergillus oryzae
1.8.3.2 ZnSO4 1 mM, 95% loss of activity Aspergillus oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.3.2 0.9
-
 L-Cys pH 7.5, 20°C Aspergillus oryzae
1.8.3.2 3.7
-
 glutathione pH 7.5, 20°C Aspergillus oryzae
1.8.3.2 4.9
-
 dithiothreitol pH 7.5, 20°C Aspergillus oryzae
1.8.3.2 7.9
-
 2-mercaptoethanol pH 7.5, 20°C Aspergillus oryzae
1.8.3.2 13.1
-
 D-Cys pH 7.5, 20°C Aspergillus oryzae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.3.2 Zn2+ 1 mM, 95% loss of activity Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.3.2 42412
-
 2 * 42412, MALDI-TOF, mature protein, 2 * 45000, SDS-PAGE Aspergillus oryzae
1.8.3.2 45000
-
 2 * 42412, MALDI-TOF, mature protein, 2 * 45000, SDS-PAGE Aspergillus oryzae
1.8.3.2 78000
-
 gel filtration Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
1.8.3.2 Aspergillus oryzae Q2U4P3
-
-
1.8.3.2 Aspergillus oryzae ATCC 42149 Q2U4P3
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.8.3.2 proteolytic modification sequence contains a signal peptide of 23 amino acids Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.3.2 2 2-mercaptoethanol + O2
-
 Aspergillus oryzae (ethyldisulfanyl)ethane + H2O2
-
 ?
1.8.3.2 2 2-mercaptoethanol + O2
-
 Aspergillus oryzae ATCC 42149 (ethyldisulfanyl)ethane + H2O2
-
 ?
1.8.3.2 2 D-Cys + O2
-
 Aspergillus oryzae D-cystine + H2O2
-
 ?
1.8.3.2 2 D-Cys + O2
-
 Aspergillus oryzae ATCC 42149 D-cystine + H2O2
-
 ?
1.8.3.2 2 dithiothreitol + O2
-
 Aspergillus oryzae dithiothreitol disulfide + H2O2
-
 ?
1.8.3.2 2 dithiothreitol + O2
-
 Aspergillus oryzae ATCC 42149 dithiothreitol disulfide + H2O2
-
 ?
1.8.3.2 2 glutathione + O2
-
 Aspergillus oryzae glutathione disulfide + H2O2
-
 ?
1.8.3.2 2 glutathione + O2
-
 Aspergillus oryzae ATCC 42149 glutathione disulfide + H2O2
-
 ?
1.8.3.2 2 L-Cys + O2
-
 Aspergillus oryzae L-cystine + H2O2
-
 ?
1.8.3.2 2 L-Cys + O2
-
 Aspergillus oryzae ATCC 42149 L-cystine + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.3.2 dimer 2 * 42412, MALDI-TOF, mature protein, 2 * 45000, SDS-PAGE Aspergillus oryzae

Synonyms

EC Number Synonyms Comment Organism
1.8.3.2 Sox2
-
 Aspergillus oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.3.2 50
-
 24 h, 42% residual activity Aspergillus oryzae
1.8.3.2 60
-
 1 h, 68% residual activity Aspergillus oryzae
1.8.3.2 75
-
 melting temperature Aspergillus oryzae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.3.2 6800
-
 L-Cys pH 7.5, 20°C Aspergillus oryzae
1.8.3.2 55400
-
 2-mercaptoethanol pH 7.5, 20°C Aspergillus oryzae
1.8.3.2 78600
-
 D-Cys pH 7.5, 20°C Aspergillus oryzae
1.8.3.2 97500
-
 glutathione pH 7.5, 20°C Aspergillus oryzae
1.8.3.2 337800
-
 dithiothreitol pH 7.5, 20°C Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.3.2 8
-
-
 Aspergillus oryzae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.8.3.2 5 8.5 more than 80% of maximum activity Aspergillus oryzae

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.3.2 FAD
-
 Aspergillus oryzae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8.3.2 5980
-
 D-Cys pH 7.5, 20°C Aspergillus oryzae
1.8.3.2 7000
-
 2-mercaptoethanol pH 7.5, 20°C Aspergillus oryzae
1.8.3.2 7600
-
 L-Cys pH 7.5, 20°C Aspergillus oryzae
1.8.3.2 26500
-
 glutathione pH 7.5, 20°C Aspergillus oryzae
1.8.3.2 69230
-
 dithiothreitol pH 7.5, 20°C Aspergillus oryzae