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Literature summary extracted from

  • Schweiger, P.; Gross, H.; Deppenmeier, U.
    Characterization of two aldo-keto reductases from Gluconobacter oxydans 621H capable of regio- and stereoselective alpha-ketocarbonyl reduction (2010), Appl. Microbiol. Biotechnol., 87, 1415-1426.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.184 expression in Escherichia coli Gluconobacter oxydans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.184 additional information not inhibitory: up to 1.0 mM Zn2+, Mg2+, Mn2+, Fe2+, or EDTA Gluconobacter oxydans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.184 0.0612
-
 NADPH pH 7.0, 25°C Gluconobacter oxydans
1.1.1.184 0.075
-
 NADPH pH 7.0, 25°C Gluconobacter oxydans
1.1.1.184 2.2
-
 methylglyoxal pH 7.0, 25°C Gluconobacter oxydans
1.1.1.184 4.8
-
 Phenylglyoxal pH 7.0, 25°C Gluconobacter oxydans
1.1.1.184 5.7
-
 2,3-Pentanedione pH 7.0, 25°C Gluconobacter oxydans
1.1.1.184 10.8
-
 2,3-Pentanedione pH 7.0, 25°C Gluconobacter oxydans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.184 31600
-
 2 * 31600, SDS-PAGE Gluconobacter oxydans
1.1.1.184 66100
-
 PAGE Gluconobacter oxydans

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.184 Gluconobacter oxydans Q5FQJ0
-
-
1.1.1.184 Gluconobacter oxydans Q5FT75
-
-
1.1.1.184 Gluconobacter oxydans 621H Q5FQJ0
-
-
1.1.1.184 Gluconobacter oxydans 621H Q5FT75
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.184 1.2
-
 substrate ethyl pyruvate, pH 7.0, 25°C Gluconobacter oxydans
1.1.1.184 3.4
-
 substrate methylglyoxal, pH 7.0, 25°C Gluconobacter oxydans
1.1.1.184 4.7
-
 substrate isoamyl pyruvate, pH 7.0, 25°C Gluconobacter oxydans
1.1.1.184 5.4
-
 substrate 1-phenyl-1,2-propanedione, pH 7.0, 25°C Gluconobacter oxydans
1.1.1.184 6
-
 substrate 2,3-butanedione, pH 7.0, 25°C Gluconobacter oxydans
1.1.1.184 6.5
-
 substrate 2,3-hexanedione, pH 7.0, 25°C Gluconobacter oxydans
1.1.1.184 17
-
 substrate 2,3-pentanedione, pH 7.0, 25°C Gluconobacter oxydans
1.1.1.184 19.1
-
 substrate phenylglyoxal, pH 7.0, 25°C Gluconobacter oxydans
1.1.1.184 35
-
 substrate methylglyoxal, pH 7.0, 25°C Gluconobacter oxydans
1.1.1.184 50.1
-
 substrate 2,3-hexanedione, pH 7.0, 25°C Gluconobacter oxydans
1.1.1.184 60
-
 substrate 2,3-butanedione, pH 7.0, 25°C Gluconobacter oxydans
1.1.1.184 63.1
-
 substrate isoamyl pyruvate, pH 7.0, 25°C Gluconobacter oxydans
1.1.1.184 65
-
 substrate 2,5-diketo-D-gluconate, pH 7.0, 25°C Gluconobacter oxydans
1.1.1.184 69.6
-
 substrate 2,3-pentanedione, pH 7.0, 25°C Gluconobacter oxydans
1.1.1.184 74.7
-
 substrate phenylglyoxal, pH 7.0, 25°C Gluconobacter oxydans
1.1.1.184 75.4
-
 substrate 1-phenyl-1,2-propanedione, pH 7.0, 25°C Gluconobacter oxydans
1.1.1.184 92.8
-
 substrate ethyl pyruvate, pH 7.0, 25°C Gluconobacter oxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.184 1-phenyl-1,2-propandione + NADPH + H+
-
 Gluconobacter oxydans (R)-1-phenyl-2-hydroxy-1-propanone + NADP+
-
 ?
1.1.1.184 1-phenyl-1,2-propandione + NADPH + H+
-
 Gluconobacter oxydans 621H (R)-1-phenyl-2-hydroxy-1-propanone + NADP+
-
 ?
1.1.1.184 1-phenyl-1,2-propandione + NADPH + H+
-
 Gluconobacter oxydans (S)-1-phenyl-2-hydroxy-1-propanone + NADP+
-
 ?
1.1.1.184 1-phenyl-1,2-propandione + NADPH + H+
-
 Gluconobacter oxydans 621H (S)-1-phenyl-2-hydroxy-1-propanone + NADP+
-
 ?
1.1.1.184 2,3-butanedione + NADPH + H+
-
 Gluconobacter oxydans ? + NADP+
-
 ?
1.1.1.184 2,3-butanedione + NADPH + H+
-
 Gluconobacter oxydans 621H ? + NADP+
-
 ?
1.1.1.184 2,3-hexanedione + NADPH + H+
-
 Gluconobacter oxydans ? + NADP+
-
 ?
1.1.1.184 2,3-hexanedione + NADPH + H+
-
 Gluconobacter oxydans 621H ? + NADP+
-
 ?
1.1.1.184 2,3-pentanedione + NADPH + H+
-
 Gluconobacter oxydans (R)-2-hydroxy-pentane-3-one + NADP+
-
 ?
1.1.1.184 2,3-pentanedione + NADPH + H+
-
 Gluconobacter oxydans 621H (R)-2-hydroxy-pentane-3-one + NADP+
-
 ?
1.1.1.184 2,3-pentanedione + NADPH + H+
-
 Gluconobacter oxydans (S)-2-hydroxy-pentane-3-one + NADP+
-
 ?
1.1.1.184 2,3-pentanedione + NADPH + H+
-
 Gluconobacter oxydans 621H (S)-2-hydroxy-pentane-3-one + NADP+
-
 ?
1.1.1.184 2,5-diketo-D-gluconate + NADPH + H+
-
 Gluconobacter oxydans ? + NADP+
-
 ?
1.1.1.184 ethyl pyruvate + NADPH + H+
-
 Gluconobacter oxydans ethyl lactate + NADP+
-
 ?
1.1.1.184 isoamyl pyruvate + NADPH + H+
-
 Gluconobacter oxydans isoamyl lactate + NADP+
-
 ?
1.1.1.184 methylglyoxal + NADPH + H+
-
 Gluconobacter oxydans ? + NADP+
-
 ?
1.1.1.184 additional information reduction of alpha-diketones is regio- and stereospecific producing the corresponding 2R-hydroxy-ketone Gluconobacter oxydans ?
-
 ?
1.1.1.184 additional information reduction of alpha-diketones is regio- and stereospecific producing the corresponding 2S-hydroxy-ketone Gluconobacter oxydans ?
-
 ?
1.1.1.184 additional information reduction of alpha-diketones is regio- and stereospecific producing the corresponding 2S-hydroxy-ketone Gluconobacter oxydans 621H ?
-
 ?
1.1.1.184 additional information reduction of alpha-diketones is regio- and stereospecific producing the corresponding 2R-hydroxy-ketone Gluconobacter oxydans 621H ?
-
 ?
1.1.1.184 phenylglyoxal + NADPH + H+
-
 Gluconobacter oxydans ? + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.184 dimer 2 * 31600, SDS-PAGE Gluconobacter oxydans

Synonyms

EC Number Synonyms Comment Organism
1.1.1.184 Gox0644
-
 Gluconobacter oxydans
1.1.1.184 Gox1615
-
 Gluconobacter oxydans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.184 35
-
-
 Gluconobacter oxydans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.184 10.7
-
 2,3-Pentanedione pH 7.0, 25°C Gluconobacter oxydans
1.1.1.184 23.8
-
 methylglyoxal pH 7.0, 25°C Gluconobacter oxydans
1.1.1.184 36.7
-
 2,3-Pentanedione pH 7.0, 25°C Gluconobacter oxydans
1.1.1.184 39.3
-
 Phenylglyoxal pH 7.0, 25°C Gluconobacter oxydans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.184 6.5
-
-
 Gluconobacter oxydans
1.1.1.184 7
-
-
 Gluconobacter oxydans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.184 0.99
-
 2,3-Pentanedione pH 7.0, 25°C Gluconobacter oxydans
1.1.1.184 6.4
-
 2,3-Pentanedione pH 7.0, 25°C Gluconobacter oxydans
1.1.1.184 8.2
-
 Phenylglyoxal pH 7.0, 25°C Gluconobacter oxydans
1.1.1.184 11
-
 methylglyoxal pH 7.0, 25°C Gluconobacter oxydans