EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.99.18 | expressed in Pichia pastoris strain X-33 | Neurospora crassa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.18 | 0.022 | - |
cellobiose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 0.043 | - |
cellopentaose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 0.045 | - |
cellotriose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 0.061 | - |
cellotetraose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 0.089 | - |
lactose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 0.196 | - |
cellopentaose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 0.204 | - |
cellotetraose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 0.23 | - |
cellotriose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 0.29 | - |
lactose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 0.81 | - |
xylotriose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 0.9 | - |
cellobiose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 1.2 | - |
maltotriose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 1.31 | - |
xylobiose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 3 | - |
xylotriose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 3.44 | - |
maltose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 3.6 | - |
xylobiose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 4.5 | - |
maltotetraose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 8.4 | - |
Mannopentaose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 17.2 | - |
maltose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 34.3 | - |
maltotriose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 35.7 | - |
maltotetraose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 39.2 | - |
Mannopentaose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 550 | - |
D-glucose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 1545 | - |
D-xylose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 2770 | - |
D-xylose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 3600 | - |
D-galactose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 3700 | - |
D-glucose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 10000 | - |
D-mannose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 13300 | - |
D-mannose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.99.18 | 85000 | - |
x * 85000, isoform CDH IIA, SDS-PAGE | Neurospora crassa |
1.1.99.18 | 85800 | - |
x * 85800, isoform CDH IIB, SDS-PAGE | Neurospora crassa |
1.1.99.18 | 110000 | 130000 | gel filtration | Neurospora crassa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.99.18 | Neurospora crassa | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.99.18 | ammonium sulfate precipitation, phenyl-Sepharose column chromatography, and Superdex 200 gel filtration | Neurospora crassa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.99.18 | cellobiose + 1,4-benzoquinone | - |
Neurospora crassa | cellobiono-1,5-lactone + reduced 1,4-benzoquinone | - |
? | |
1.1.99.18 | cellobiose + 2,6-dichloroindophenol | - |
Neurospora crassa | cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol | - |
? | |
1.1.99.18 | cellobiose + cytochrome c | - |
Neurospora crassa | cellobiono-1,5-lactone + reduced cytochrome c | - |
? | |
1.1.99.18 | cellopentaose + 1,4-benzoquinone | - |
Neurospora crassa | ? + reduced 1,4-benzoquinone | - |
? | |
1.1.99.18 | cellotetraose + 1,4-benzoquinone | - |
Neurospora crassa | ? + reduced 1,4-benzoquinone | - |
? | |
1.1.99.18 | cellotriose + 1,4-benzoquinone | - |
Neurospora crassa | ? + reduced 1,4-benzoquinone | - |
? | |
1.1.99.18 | cellulose + ferrocenium hexafluorophosphate | - |
Neurospora crassa | cellobiono-1,5-lactone + ferricenium hexafluorophosphate | - |
? | |
1.1.99.18 | D-galactose + 1,4-benzoquinone | - |
Neurospora crassa | ? + reduced 1,4-benzoquinone | - |
? | |
1.1.99.18 | D-glucose + 1,4-benzoquinone | - |
Neurospora crassa | ? + reduced 1,4-benzoquinone | - |
? | |
1.1.99.18 | D-mannose + 1,4-benzoquinone | - |
Neurospora crassa | ? + reduced 1,4-benzoquinone | - |
? | |
1.1.99.18 | D-xylose + 1,4-benzoquinone | - |
Neurospora crassa | ? + reduced 1,4-benzoquinone | - |
? | |
1.1.99.18 | lactose + 1,4-benzoquinone | - |
Neurospora crassa | ? + reduced 1,4-benzoquinone | - |
? | |
1.1.99.18 | maltose + 1,4-benzoquinone | - |
Neurospora crassa | ? + reduced 1,4-benzoquinone | - |
? | |
1.1.99.18 | maltotetraose + 1,4-benzoquinone | - |
Neurospora crassa | ? + reduced 1,4-benzoquinone | - |
? | |
1.1.99.18 | maltotriose + 1,4-benzoquinone | - |
Neurospora crassa | ? + reduced 1,4-benzoquinone | - |
? | |
1.1.99.18 | mannopentaose + 1,4-benzoquinone | - |
Neurospora crassa | ? + reduced 1,4-benzoquinone | - |
? | |
1.1.99.18 | additional information | isoform CDH IIA exhibits 4 to 5times higher apparent catalytic constants (kcat and Km values) than isoform CDH IIB for most tested carbohydrates | Neurospora crassa | ? | - |
? | |
1.1.99.18 | xylobiose + 1,4-benzoquinone | - |
Neurospora crassa | 4-O-beta-D-xylopyranosyl-D-xylono-1,5-lactone + reduced 1,4-benzoquinone | - |
? | |
1.1.99.18 | xylotriose + 1,4-benzoquinone | - |
Neurospora crassa | ? + reduced 1,4-benzoquinone | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.99.18 | ? | x * 85000, isoform CDH IIA, SDS-PAGE | Neurospora crassa |
1.1.99.18 | ? | x * 85800, isoform CDH IIB, SDS-PAGE | Neurospora crassa |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.99.18 | CDH IIA | isoform | Neurospora crassa |
1.1.99.18 | CDH IIB | isoform | Neurospora crassa |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.18 | 0.4 | - |
maltose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 1 | - |
maltotriose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 1.4 | - |
maltotetraose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 1.5 | - |
D-galactose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 1.5 | - |
D-xylose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 1.8 | - |
maltotriose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 1.9 | - |
D-xylose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 2.2 | - |
maltotetraose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 2.3 | - |
xylotriose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 3.5 | - |
maltose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 3.6 | - |
D-galactose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 4.3 | - |
D-mannose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 4.7 | - |
D-mannose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 5.8 | - |
xylobiose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 6 | - |
xylotriose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 7.4 | - |
Mannopentaose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 8.1 | - |
D-glucose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 10.2 | - |
cellopentaose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 11.4 | - |
cellobiose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 11.6 | - |
cellotetraose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 11.8 | - |
lactose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 12.4 | - |
cellotriose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 19.8 | - |
Mannopentaose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 45.4 | - |
xylobiose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 45.8 | - |
cellobiose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 46 | - |
cellotetraose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 46.6 | - |
cellopentaose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 49.3 | - |
lactose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 49.7 | - |
cellotriose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 55 | - |
D-glucose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.99.18 | 5 | - |
using 2,6-dichlorophenolindol as electron donor | Neurospora crassa |
1.1.99.18 | 7 | - |
using 1,4-benzoquinone as electron donor | Neurospora crassa |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.99.18 | 3.7 | 8 | more than 50% relative activity from pH 3.8 to 6.5 using 1,4-benzoquinone as electron acceptor | Neurospora crassa |
1.1.99.18 | 3.8 | 6.5 | more than 50% relative activity from pH 3.8 to 6.5 using 2,6-dichlorophenolindol as electron acceptor | Neurospora crassa |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.1.99.18 | Neurospora crassa | isoform CDH IIB, isoelectric focusing | - |
4.9 |
1.1.99.18 | Neurospora crassa | isoform CDH IIA, isoelectric focusing | - |
5.1 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.18 | 0.0003 | - |
D-mannose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 0.0004 | - |
D-mannose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 0.0005 | - |
D-xylose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 0.0012 | - |
D-xylose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 0.014 | - |
D-glucose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 0.015 | - |
D-glucose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 0.023 | - |
maltose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 0.053 | - |
maltotriose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 0.061 | - |
maltotetraose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 0.506 | - |
Mannopentaose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 0.8 | - |
maltotriose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 0.88 | - |
Mannopentaose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 1.02 | - |
maltose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 2 | - |
xylotriose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 2.74 | - |
maltotetraose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 2.8 | - |
xylotriose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 4.45 | - |
xylobiose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 12.8 | - |
xylobiose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 133 | - |
lactose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 170 | - |
lactose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 189 | - |
cellotetraose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 216 | - |
cellotriose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 226 | - |
cellotetraose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 238 | - |
cellopentaose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 238 | - |
cellopentaose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 278 | - |
cellotriose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 508 | - |
cellobiose | isoform CDH IIA, at pH 6.0 and 30°C | Neurospora crassa | |
1.1.99.18 | 527 | - |
cellobiose | isoform CDH IIB, at pH 6.0 and 30°C | Neurospora crassa |