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Literature summary extracted from

  • Kaushik, G.; Thakur, I.
    Purification, characterization and usage of thermotolerant laccase from Bacillus sp. for biodegradation of synthetic dyes (2013), Appl. Biochem. Microbiol., 49, 352-359.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.10.3.2 additional information usage of thermotolerant laccase from Bacillus sp. for biodegradation of synthetic dyes, overview Bacillus sp. (in: Bacteria)

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) shows substrate inhibition at high concentrations above 6 mM Bacillus sp. (in: Bacteria)
1.10.3.2 acetonitrile completely inhibits enzyme activity Bacillus sp. (in: Bacteria)
1.10.3.2 EDTA 28% inhibition at 40 mM Bacillus sp. (in: Bacteria)
1.10.3.2 methanol completely inhibits enzyme activity Bacillus sp. (in: Bacteria)
1.10.3.2 Sodium azide completely inhibits enzyme activity Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 additional information
-
additional information Michaelis-Menten kinetics Bacillus sp. (in: Bacteria)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.2 extracellular
-
Bacillus sp. (in: Bacteria)
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 70000
-
x * 70000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Bacillus sp. (in: Bacteria)
-
isolated from distillery effluent and sludge
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 native extracellular enzyme 6.6fold from culture supernatant by anion exchange chromatography Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 952
-
puriifed native extracellular enzyme, pH 3.0, 22°C Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Bacillus sp. (in: Bacteria) ?
-
?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 70000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 35
-
-
Bacillus sp. (in: Bacteria)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.2 25 75 activity range Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 25
-
purified native enzyme, half-life is 116 min Bacillus sp. (in: Bacteria)
1.10.3.2 35
-
purified native enzyme, half-life is 104 min Bacillus sp. (in: Bacteria)
1.10.3.2 45
-
purified native enzyme, half-life is 67 min Bacillus sp. (in: Bacteria)
1.10.3.2 55
-
purified native enzyme, half-life is 47 min Bacillus sp. (in: Bacteria)
1.10.3.2 65
-
purified native enzyme, half-life is 33 min Bacillus sp. (in: Bacteria)
1.10.3.2 75
-
purified native enzyme, half-life is 23 min Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 3
-
-
Bacillus sp. (in: Bacteria)