EC Number | Application | Comment | Organism |
---|---|---|---|
1.10.3.2 | additional information | usage of thermotolerant laccase from Bacillus sp. for biodegradation of synthetic dyes, overview | Bacillus sp. (in: Bacteria) |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.10.3.2 | 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | shows substrate inhibition at high concentrations above 6 mM | Bacillus sp. (in: Bacteria) | |
1.10.3.2 | acetonitrile | completely inhibits enzyme activity | Bacillus sp. (in: Bacteria) | |
1.10.3.2 | EDTA | 28% inhibition at 40 mM | Bacillus sp. (in: Bacteria) | |
1.10.3.2 | methanol | completely inhibits enzyme activity | Bacillus sp. (in: Bacteria) | |
1.10.3.2 | Sodium azide | completely inhibits enzyme activity | Bacillus sp. (in: Bacteria) |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.10.3.2 | additional information | - |
additional information | Michaelis-Menten kinetics | Bacillus sp. (in: Bacteria) |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.10.3.2 | extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 70000 | - |
x * 70000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.10.3.2 | Bacillus sp. (in: Bacteria) | - |
isolated from distillery effluent and sludge | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.10.3.2 | native extracellular enzyme 6.6fold from culture supernatant by anion exchange chromatography | Bacillus sp. (in: Bacteria) |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 952 | - |
puriifed native extracellular enzyme, pH 3.0, 22°C | Bacillus sp. (in: Bacteria) |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.10.3.2 | 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Bacillus sp. (in: Bacteria) | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.10.3.2 | ? | x * 70000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 35 | - |
- |
Bacillus sp. (in: Bacteria) |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 25 | 75 | activity range | Bacillus sp. (in: Bacteria) |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 25 | - |
purified native enzyme, half-life is 116 min | Bacillus sp. (in: Bacteria) |
1.10.3.2 | 35 | - |
purified native enzyme, half-life is 104 min | Bacillus sp. (in: Bacteria) |
1.10.3.2 | 45 | - |
purified native enzyme, half-life is 67 min | Bacillus sp. (in: Bacteria) |
1.10.3.2 | 55 | - |
purified native enzyme, half-life is 47 min | Bacillus sp. (in: Bacteria) |
1.10.3.2 | 65 | - |
purified native enzyme, half-life is 33 min | Bacillus sp. (in: Bacteria) |
1.10.3.2 | 75 | - |
purified native enzyme, half-life is 23 min | Bacillus sp. (in: Bacteria) |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 3 | - |
- |
Bacillus sp. (in: Bacteria) |