EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.99.B2 | expressed in Escherichia coli BL-21 plysS (DE3) cells | Pseudomonas fluorescens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.99.B2 | 35 | - |
L-proline | at pH 8.5 and 30°C | Pseudomonas fluorescens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.99.B2 | 40000 | - |
gel filtration | Pseudomonas fluorescens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.99.B2 | Pseudomonas fluorescens | - |
- |
- |
1.5.99.B2 | Pseudomonas fluorescens Pf-5 | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.99.B2 | glycine + 2,6-dichlorophenolindophenol + H2O | 52% activity compared to L-proline | Pseudomonas fluorescens | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.5.99.B2 | glycine + 2,6-dichlorophenolindophenol + H2O | 52% activity compared to L-proline | Pseudomonas fluorescens Pf-5 | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.5.99.B2 | L-alanine + 2,6-dichlorophenolindophenol + H2O | 48% activity compared to L-proline | Pseudomonas fluorescens | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.5.99.B2 | L-alanine + 2,6-dichlorophenolindophenol + H2O | 48% activity compared to L-proline | Pseudomonas fluorescens Pf-5 | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.5.99.B2 | L-arginine + 2,6-dichlorophenolindophenol + H2O | 62% activity compared to L-proline | Pseudomonas fluorescens | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.5.99.B2 | L-arginine + 2,6-dichlorophenolindophenol + H2O | 62% activity compared to L-proline | Pseudomonas fluorescens Pf-5 | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.5.99.B2 | L-histidine + 2,6-dichlorophenolindophenol + H2O | 72% activity compared to L-proline | Pseudomonas fluorescens | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.5.99.B2 | L-histidine + 2,6-dichlorophenolindophenol + H2O | 72% activity compared to L-proline | Pseudomonas fluorescens Pf-5 | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.5.99.B2 | L-hydroxyproline + 2,6-dichlorophenolindophenol + H2O | 100% activity compared to L-proline | Pseudomonas fluorescens | DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.5.99.B2 | L-leucine + 2,6-dichlorophenolindophenol + H2O | 42% activity compared to L-proline | Pseudomonas fluorescens | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.5.99.B2 | L-proline + 2,6-dichlorophenolindophenol + H2O | 100% activity | Pseudomonas fluorescens | DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.5.99.B2 | L-proline + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium | - |
Pseudomonas fluorescens | DELTA1-pyrroline-5-carboxylate + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium | - |
? | |
1.5.99.B2 | L-serine + 2,6-dichlorophenolindophenol + H2O | 55% activity compared to L-proline | Pseudomonas fluorescens | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.5.99.B2 | L-threonine + 2,6-dichlorophenolindophenol + H2O | 66% activity compared to L-proline | Pseudomonas fluorescens | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.5.99.B2 | L-valine + 2,6-dichlorophenolindophenol + H2O | 33% activity compared to L-proline | Pseudomonas fluorescens | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.5.99.B2 | additional information | no activity with D-proline, L-tryptophan, L-glutamate, and L-aspartate | Pseudomonas fluorescens | ? | - |
? | |
1.5.99.B2 | additional information | no activity with D-proline, L-tryptophan, L-glutamate, and L-aspartate | Pseudomonas fluorescens Pf-5 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.99.B2 | monomer | 1 * 40000, SDS-PAGE | Pseudomonas fluorescens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.99.B2 | L-proline: FAD oxidoreductase | - |
Pseudomonas fluorescens |
1.5.99.B2 | PRODH | - |
Pseudomonas fluorescens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.99.B2 | 30 | - |
- |
Pseudomonas fluorescens |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.99.B2 | 20 | 40 | more than 60% activity between 20-40°C, a sharp decrease is observed above 30°C and enzyme activity is completely inactivated at 70°C | Pseudomonas fluorescens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.99.B2 | 8.5 | - |
- |
Pseudomonas fluorescens |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.5.99.B2 | 6 | 11 | more than 50% activity between pH 6.0 and 11.0 | Pseudomonas fluorescens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.99.B2 | FAD | - |
Pseudomonas fluorescens |