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Literature summary extracted from

  • Mohammadi, H.; Omidinia, E.
    Proline dehydrogenase from Pseudomonas fluorescens: Gene cloning, purification, characterization and homology modeling (2012), Appl. Biochem. Microbiol., 48, 167-174.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.99.B2 expressed in Escherichia coli BL-21 plysS (DE3) cells Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.99.B2 35
-
L-proline at pH 8.5 and 30°C Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.99.B2 40000
-
gel filtration Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
1.5.99.B2 Pseudomonas fluorescens
-
-
-
1.5.99.B2 Pseudomonas fluorescens Pf-5
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.99.B2 glycine + 2,6-dichlorophenolindophenol + H2O 52% activity compared to L-proline Pseudomonas fluorescens ? + reduced 2,6-dichlorophenolindophenol
-
?
1.5.99.B2 glycine + 2,6-dichlorophenolindophenol + H2O 52% activity compared to L-proline Pseudomonas fluorescens Pf-5 ? + reduced 2,6-dichlorophenolindophenol
-
?
1.5.99.B2 L-alanine + 2,6-dichlorophenolindophenol + H2O 48% activity compared to L-proline Pseudomonas fluorescens ? + reduced 2,6-dichlorophenolindophenol
-
?
1.5.99.B2 L-alanine + 2,6-dichlorophenolindophenol + H2O 48% activity compared to L-proline Pseudomonas fluorescens Pf-5 ? + reduced 2,6-dichlorophenolindophenol
-
?
1.5.99.B2 L-arginine + 2,6-dichlorophenolindophenol + H2O 62% activity compared to L-proline Pseudomonas fluorescens ? + reduced 2,6-dichlorophenolindophenol
-
?
1.5.99.B2 L-arginine + 2,6-dichlorophenolindophenol + H2O 62% activity compared to L-proline Pseudomonas fluorescens Pf-5 ? + reduced 2,6-dichlorophenolindophenol
-
?
1.5.99.B2 L-histidine + 2,6-dichlorophenolindophenol + H2O 72% activity compared to L-proline Pseudomonas fluorescens ? + reduced 2,6-dichlorophenolindophenol
-
?
1.5.99.B2 L-histidine + 2,6-dichlorophenolindophenol + H2O 72% activity compared to L-proline Pseudomonas fluorescens Pf-5 ? + reduced 2,6-dichlorophenolindophenol
-
?
1.5.99.B2 L-hydroxyproline + 2,6-dichlorophenolindophenol + H2O 100% activity compared to L-proline Pseudomonas fluorescens DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
?
1.5.99.B2 L-leucine + 2,6-dichlorophenolindophenol + H2O 42% activity compared to L-proline Pseudomonas fluorescens ? + reduced 2,6-dichlorophenolindophenol
-
?
1.5.99.B2 L-proline + 2,6-dichlorophenolindophenol + H2O 100% activity Pseudomonas fluorescens DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
?
1.5.99.B2 L-proline + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium
-
Pseudomonas fluorescens DELTA1-pyrroline-5-carboxylate + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium
-
?
1.5.99.B2 L-serine + 2,6-dichlorophenolindophenol + H2O 55% activity compared to L-proline Pseudomonas fluorescens ? + reduced 2,6-dichlorophenolindophenol
-
?
1.5.99.B2 L-threonine + 2,6-dichlorophenolindophenol + H2O 66% activity compared to L-proline Pseudomonas fluorescens ? + reduced 2,6-dichlorophenolindophenol
-
?
1.5.99.B2 L-valine + 2,6-dichlorophenolindophenol + H2O 33% activity compared to L-proline Pseudomonas fluorescens ? + reduced 2,6-dichlorophenolindophenol
-
?
1.5.99.B2 additional information no activity with D-proline, L-tryptophan, L-glutamate, and L-aspartate Pseudomonas fluorescens ?
-
?
1.5.99.B2 additional information no activity with D-proline, L-tryptophan, L-glutamate, and L-aspartate Pseudomonas fluorescens Pf-5 ?
-
?

Subunits

EC Number Subunits Comment Organism
1.5.99.B2 monomer 1 * 40000, SDS-PAGE Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
1.5.99.B2 L-proline: FAD oxidoreductase
-
Pseudomonas fluorescens
1.5.99.B2 PRODH
-
Pseudomonas fluorescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.99.B2 30
-
-
Pseudomonas fluorescens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.5.99.B2 20 40 more than 60% activity between 20-40°C, a sharp decrease is observed above 30°C and enzyme activity is completely inactivated at 70°C Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.99.B2 8.5
-
-
Pseudomonas fluorescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.99.B2 6 11 more than 50% activity between pH 6.0 and 11.0 Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.99.B2 FAD
-
Pseudomonas fluorescens