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Literature summary extracted from

  • Li, J.; Liu, C.; Li, B.; Yuan, H.; Yang, J.; Zheng, B.
    Identification and molecular characterization of a novel DyP-type peroxidase from Pseudomonas aeruginosa PKE117 (2012), Appl. Biochem. Biotechnol., 166, 774-785.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.19 expression in Escherichia coli Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.19 Fe3+ 10 mM, 33% residual activity Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.19 0.107
-
Reactive Blue 5 pH 3.5, 30°C Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.19 Al3+ 10 mM, 117% of initial activity Pseudomonas aeruginosa
1.11.1.19 Co2+ 10 mM, 115% of initial activity Pseudomonas aeruginosa
1.11.1.19 Zn2+ 10 mM, 122% of initial activity Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.19 32000
-
2 * 32000, SDS-PAGE Pseudomonas aeruginosa
1.11.1.19 64000
-
gel filtration Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.19 Pseudomonas aeruginosa D5LRR6
-
-
1.11.1.19 Pseudomonas aeruginosa PKE117 D5LRR6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.19 2,6-dimethoxyphenol + H2O2 + H+ 18.3% of the activity with Reactive Blue 5 Pseudomonas aeruginosa ?
-
?
1.11.1.19 2,6-dimethoxyphenol + H2O2 + H+ 18.3% of the activity with Reactive Blue 5 Pseudomonas aeruginosa PKE117 ?
-
?
1.11.1.19 Reactive Black 5 + H2O2 16.4% of the activity with Reactive Blue 5 Pseudomonas aeruginosa oxidized Reactive Black 5 + H2O
-
?
1.11.1.19 Reactive Black 5 + H2O2 16.4% of the activity with Reactive Blue 5 Pseudomonas aeruginosa PKE117 oxidized Reactive Black 5 + H2O
-
?
1.11.1.19 Reactive Blue 19 + H2O2 19% of the activity with Reactive Blue 5 Pseudomonas aeruginosa oxidized Reactive Blue 5 + H2O
-
?
1.11.1.19 Reactive Blue 19 + H2O2 19% of the activity with Reactive Blue 5 Pseudomonas aeruginosa PKE117 oxidized Reactive Blue 5 + H2O
-
?
1.11.1.19 Reactive Blue 5 + H2O2
-
Pseudomonas aeruginosa phthalate + 2,2'-disulfonyl azobenzene + 3-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]benzenesulfonate
-
?
1.11.1.19 Reactive Blue 5 + H2O2
-
Pseudomonas aeruginosa PKE117 phthalate + 2,2'-disulfonyl azobenzene + 3-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]benzenesulfonate
-
?
1.11.1.19 Vat Blue 6 + H2O2 5.1% of the activity with Reactive Blue 5 Pseudomonas aeruginosa oxidized Vat Blue 6 + H2O
-
?
1.11.1.19 Vat Blue 6 + H2O2 5.1% of the activity with Reactive Blue 5 Pseudomonas aeruginosa PKE117 oxidized Vat Blue 6 + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.11.1.19 dimer 2 * 32000, SDS-PAGE Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
1.11.1.19 DyP
-
Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.19 60
-
4 h, 83% residual activity Pseudomonas aeruginosa
1.11.1.19 70
-
1 h, more than 70% residual activity Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.19 0.0235
-
Reactive Blue 5 pH 3.5, 30°C Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.19 3.5
-
-
Pseudomonas aeruginosa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.1.19 4
-
rapid loss of activity below Pseudomonas aeruginosa
1.11.1.19 6.5 7 30 min, stable Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.19 heme enzyme displays typical heme absorbance of Soret band, with an Rz value of 1.18 Pseudomonas aeruginosa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.19 Pseudomonas aeruginosa calculated
-
4.6

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.19 0.219
-
Reactive Blue 5 pH 3.5, 30°C Pseudomonas aeruginosa