EC Number | Application | Comment | Organism |
---|---|---|---|
3.5.1.1 | analysis | biosensor for asparagine using a thermostable recombinant asparaginase from Archaeoglobus fulgidus immobilized in front of an ammonium-selective electrode. The biosensor has a detection limit of 0.06 mM for L-asparagine. It shows high stability | Archaeoglobus fulgidus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.1.1 | expression in Escherichia coli as a fusion protein with a polyhistidine tail | Archaeoglobus fulgidus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.1 | 0.005 | - |
L-asparagine | pH 9.2, 70°C, recombinant enzyme | Archaeoglobus fulgidus | |
3.5.1.1 | 0.08 | - |
L-asparagine | pH 9.2, 37°C, recombinant enzyme | Archaeoglobus fulgidus | |
3.5.1.1 | 0.2 | - |
L-glutamine | pH 9.2, 70°C, recombinant enzyme | Archaeoglobus fulgidus | |
3.5.1.1 | 0.35 | - |
L-glutamine | pH 9.2, 37°C, recombinant enzyme | Archaeoglobus fulgidus | |
3.5.1.1 | 1.2 | - |
D-asparagine | pH 9.2, 70°C, recombinant enzyme | Archaeoglobus fulgidus | |
3.5.1.1 | 1.8 | - |
D-asparagine | pH 9.2, 37°C, recombinant enzyme | Archaeoglobus fulgidus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.1 | Archaeoglobus fulgidus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.1 | after heat treatment to denature most of the native Escherichia coli proteins, the enzyme is purified by an immobilized metal ion affinity chromatography method | Archaeoglobus fulgidus |
EC Number | Storage Stability | Organism |
---|---|---|
3.5.1.1 | 22°C, pH 9.2, 4 months, the enzyme retains about 90% of enzyme activity | Archaeoglobus fulgidus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.1 | D-asparagine + H2O | catalytic activity of the recombinant enzyme for L-asparagine is 5fold higher than for D-asparagine | Archaeoglobus fulgidus | D-aspartate + NH3 | - |
? | |
3.5.1.1 | L-asparagine + H2O | catalytic activity of the recombinant enzyme for L-asparagine is 5fold higher than for D-asparagine | Archaeoglobus fulgidus | L-aspartate + NH3 | - |
? | |
3.5.1.1 | L-glutamine + H2O | recombinant enzyme has 4fold higher activity for L-asparagine than for L-glutamine | Archaeoglobus fulgidus | L-glutamate + NH3 | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.1 | 80 | - |
5 min, stable | Archaeoglobus fulgidus |
3.5.1.1 | 85 | - |
5 min, 30% loss of activity | Archaeoglobus fulgidus |
3.5.1.1 | 95 | - |
5 min, 90% loss of activity | Archaeoglobus fulgidus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.1 | 9.2 | - |
- |
Archaeoglobus fulgidus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.1 | 8 | 10 | pH 8.0: about 70% of maximal activity, pH 10.0: about 85% of maximal activity | Archaeoglobus fulgidus |