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Literature summary extracted from

  • Pi, N.; Hoang, M.B.; Gao, H.; Mougous, J.D.; Bertozzi, C.R.; Leary, J.A.
    Kinetic measurements and mechanism determination of Stf0 sulfotransferase using mass spectrometry (2005), Anal. Biochem., 341, 94-104.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.37 adenosine 3',5'-bisphosphate good competitive inhibitor with regard to 3'-phosphoadenylyl sulfate. Very weak noncompetitive inhibitor with reagrd to trehalose Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.37 0.018
-
 3'-phosphoadenylyl sulfate pH 7.5, 22°C Mycobacterium tuberculosis
2.8.2.37 15
-
 alpha,alpha-trehalose pH 7.5, 22°C Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.37 3'-phosphoadenylyl sulfate + alpha,alpha-trehalose Mycobacterium tuberculosis the sulfation of trehalose is required for the biosynthesis of sulfolipid-1 adenosine 3',5'-bisphosphate + 2-O-sulfo-alpha,alpha-trehalose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.37 Mycobacterium tuberculosis O53699
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.8.2.37 3'-phosphoadenylyl sulfate + alpha,alpha-trehalose = adenosine 3',5'-bisphosphate + 2-O-sulfo-alpha,alpha-trehalose rapid equilibrium random sequential Bi-Bi mechanism with formation of a ternary complex intermediate Mycobacterium tuberculosis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.37 3'-phosphoadenylyl sulfate + alpha,alpha-trehalose
-
 Mycobacterium tuberculosis adenosine 3',5'-bisphosphate + 2-O-sulfo-alpha,alpha-trehalose
-
 ?
2.8.2.37 3'-phosphoadenylyl sulfate + alpha,alpha-trehalose the sulfation of trehalose is required for the biosynthesis of sulfolipid-1 Mycobacterium tuberculosis adenosine 3',5'-bisphosphate + 2-O-sulfo-alpha,alpha-trehalose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.2.37 Stf0 sulfotransferase
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8.2.37 1.6
-
 3'-phosphoadenylyl sulfate pH 7.5, 22°C Mycobacterium tuberculosis
2.8.2.37 2.23
-
 alpha,alpha-trehalose pH 7.5, 22°C Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8.2.37 0.018
-
 adenosine 3',5'-bisphosphate pH 7.5, 22°C, competitive inhibitor with regard to 3'-phosphoadenylyl sulfate Mycobacterium tuberculosis
2.8.2.37 0.405
-
 adenosine 3',5'-bisphosphate pH 7.5, 22°C, noncompetitive inhibitor with regard to trehalose Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
2.8.2.37 physiological function the sulfation of trehalose is required for the biosynthesis of sulfolipid-1 Mycobacterium tuberculosis