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Literature summary extracted from

  • Di Fusco, M.; Federico, R.; Boffi, A.; Macone, A.; Favero, G.; Mazzei, F.
    Characterization and application of a diamine oxidase from Lathyrus sativus as component of an electrochemical biosensor for the determination of biogenic amines in wine and beer (2011), Anal. Bioanal. Chem., 401, 707-716.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.4.3.22 analysis the enzyme can be used as biocatalytic component of an electrochemical biosensor for the determination of biogenic amines index in wine and beer samples Lathyrus sativus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.22 0.0587
-
spermine free enzyme, in 0.1 M phosphate buffer, pH 7.4, at 25°C Lathyrus sativus
1.4.3.22 0.1
-
spermidine free enzyme, in 0.1 M phosphate buffer, pH 7.4, at 25°C Lathyrus sativus
1.4.3.22 0.112
-
histamine free enzyme, in 0.1 M phosphate buffer, pH 7.4, at 25°C Lathyrus sativus
1.4.3.22 0.172
-
cadaverine free enzyme, in 0.1 M phosphate buffer, pH 7.4, at 25°C Lathyrus sativus
1.4.3.22 0.219
-
putrescine free enzyme, in 0.1 M phosphate buffer, pH 7.4, at 25°C Lathyrus sativus
1.4.3.22 0.53
-
tyramine immobilized enzyme, apparent value, in 0.1 M phosphate buffer, pH 7.4, at 25°C Lathyrus sativus
1.4.3.22 0.84
-
Phenylethylamine immobilized enzyme, apparent value, in 0.1 M phosphate buffer, pH 7.4, at 25°C Lathyrus sativus
1.4.3.22 1.05
-
histamine immobilized enzyme, apparent value, in 0.1 M phosphate buffer, pH 7.4, at 25°C Lathyrus sativus
1.4.3.22 1.14
-
spermine immobilized enzyme, apparent value, in 0.1 M phosphate buffer, pH 7.4, at 25°C Lathyrus sativus
1.4.3.22 1.58
-
putrescine immobilized enzyme, apparent value, in 0.1 M phosphate buffer, pH 7.4, at 25°C Lathyrus sativus
1.4.3.22 1.77
-
spermidine immobilized enzyme, apparent value, in 0.1 M phosphate buffer, pH 7.4, at 25°C Lathyrus sativus
1.4.3.22 2.84
-
cadaverine immobilized enzyme, apparent value, in 0.1 M phosphate buffer, pH 7.4, at 25°C Lathyrus sativus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.22 148000
-
x * 148000 Lathyrus sativus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.22 histamine + H2O + O2 Lathyrus sativus
-
(imidazol-4-yl)acetaldehyde + NH3 + H2O2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.22 Lathyrus sativus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.22 cadaverine + H2O + O2
-
Lathyrus sativus 5-aminopentanal + NH3 + H2O2
-
?
1.4.3.22 histamine + H2O + O2
-
Lathyrus sativus (imidazol-4-yl)acetaldehyde + NH3 + H2O2
-
?
1.4.3.22 phenylethylamine + H2O + O2
-
Lathyrus sativus phenylacetaldehyde + NH3 + H2O2
-
?
1.4.3.22 putrescine + H2O + O2
-
Lathyrus sativus ? + NH3 + H2O2
-
?
1.4.3.22 spermidine + H2O + O2
-
Lathyrus sativus ? + NH3 + H2O2
-
?
1.4.3.22 spermine + H2O + O2
-
Lathyrus sativus ? + NH3 + H2O2
-
?
1.4.3.22 tyramine + H2O + O2
-
Lathyrus sativus ? + NH3 + H2O2
-
?

Subunits

EC Number Subunits Comment Organism
1.4.3.22 ? x * 148000 Lathyrus sativus

Synonyms

EC Number Synonyms Comment Organism
1.4.3.22 DAO
-
Lathyrus sativus