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Literature summary extracted from

  • Rohman, A.; van Oosterwijk, N.; Dijkstra, B.W.
    Purification, crystallization and preliminary X-ray crystallographic analysis of 3-ketosteroid DELTA1-dehydrogenase from Rhodococcus erythropolis SQ1 (2012), Acta Crystallogr. Sect. F, 68, 551-556.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.99.4 gene kstD1, heterologous expression of the His-tagged DELTA1-KSTD1 protein in Escherichia coli strain BL21(DE3) Rhodococcus erythropolis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.99.4 sitting drop vapour diffusion method, mixing of 15 mg/ml protein in 25 mM bicine, pH 9.0, 100 mM NaCl, 10% v/v glycerol with 2% v/v PEG 400, 0.1 M, HEPES, pH 7.5, 2.0 M ammonium sulfate, 20°C, 5-7 days, method optimization, X-ray diffraction structure determination and analysis at 2.0 A resolution, multiwavelength anomalous dispersion analysis Rhodococcus erythropolis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.99.4 56000
-
x * 56000 Rhodococcus erythropolis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.99.4 4-androstene-3,17-dione + FAD Rhodococcus erythropolis
-
1,4-androstadiene-3,17-dione + FADH2
-
?
1.3.99.4 4-androstene-3,17-dione + FAD Rhodococcus erythropolis SQ1
-
1,4-androstadiene-3,17-dione + FADH2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.4 Rhodococcus erythropolis Q9RA02 three 3-ketosteroid DELTA1-dehydrogenase isoenzymes, gene kstD1 encodes isozyme DELTA1-KSTD1
-
1.3.99.4 Rhodococcus erythropolis SQ1 Q9RA02 three 3-ketosteroid DELTA1-dehydrogenase isoenzymes, gene kstD1 encodes isozyme DELTA1-KSTD1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.99.4 recombinant His-tagged DELTA1-KSTD1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Rhodococcus erythropolis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.4 4-androstene-3,17-dione + FAD
-
Rhodococcus erythropolis 1,4-androstadiene-3,17-dione + FADH2
-
?
1.3.99.4 4-androstene-3,17-dione + FAD
-
Rhodococcus erythropolis SQ1 1,4-androstadiene-3,17-dione + FADH2
-
?

Subunits

EC Number Subunits Comment Organism
1.3.99.4 ? x * 56000 Rhodococcus erythropolis

Synonyms

EC Number Synonyms Comment Organism
1.3.99.4 3-ketosteroid delta1-dehydrogenase
-
Rhodococcus erythropolis
1.3.99.4 DELTA1-KSTD1
-
Rhodococcus erythropolis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.3.99.4 6 9 below pH 6.0 no apparent Tm is observed, indicating that DELTA1-KSTD1 is destabilized at low pH. The higher the pH value, within the range of pH 6.0-9.0, the higher the apparent Tm of DELTA1-KSTD1. At pH 9.0 the apparent Tm of DELTA1-KSTD1 is about 7 K higher than at pH 7.0 Rhodococcus erythropolis

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.99.4 FAD prosthetic group, flavoenzyme Rhodococcus erythropolis

General Information

EC Number General Information Comment Organism
1.3.99.4 physiological function 3-ketosteroid DELTA1-dehydrogenase plays a crucial role in the early steps of steroid degradation by introducing a double bond between the C1 and C2 atoms of the A-ring of its 3-ketosteroid substrates Rhodococcus erythropolis