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Literature summary extracted from

  • Park, S.; Alli, I.; Park, K.; Oh, B.; Lee, B.
    Mutation studies in the active site of beta-glycosidase from Pyrococcus furiosus DSM 3638 (2013), Protein Pept. Lett., 20, 107-114.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.B28 expression in Escherichia coli, mutant enzymes Q150W and Q77R/Q150W Pyrococcus furiosus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.B28 Q150W the mutant enzyme has more affinity (Km) towards each substrate but shows lower turnover number (kcat) compared to the wild-type enzyme Pyrococcus furiosus
3.2.1.B28 Q77R/Q150W the mutant enzyme has more affinity (Km) towards each substrate but shows lower turnover number (kcat) compared to the wild-type enzyme Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.B28 0.01
-
 lactose pH 5.0, 95°C, mutant enzyme Q77R/Q150W Pyrococcus furiosus
3.2.1.B28 0.02
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 95°C, mutant enzyme Q77R/Q150W Pyrococcus furiosus
3.2.1.B28 0.03
-
 lactose pH 5.0, 95°C, mutant enzyme Q150W Pyrococcus furiosus
3.2.1.B28 0.03
-
 4-nitrophenyl beta-D-mannopyranoside pH 5.0, 95°C, mutant enzyme Q77R/Q150W Pyrococcus furiosus
3.2.1.B28 0.04
-
 cellobiose pH 5.0, 95°C, mutant enzyme Q77R/Q150W Pyrococcus furiosus
3.2.1.B28 0.05
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 95°C, mutant enzyme Q150W Pyrococcus furiosus
3.2.1.B28 0.05
-
 4-nitrophenyl beta-D-mannopyranoside pH 5.0, 95°C, mutant enzyme Q150W Pyrococcus furiosus
3.2.1.B28 0.1
-
 2-nitrophenyl beta-D-glucopyranoside pH 5.0, 95°C, mutant enzyme Q77R/Q150W Pyrococcus furiosus
3.2.1.B28 0.3
-
 2-nitrophenyl beta-D-glucopyranoside pH 5.0, 95°C, mutant enzyme Q150W Pyrococcus furiosus
3.2.1.B28 0.38
-
 cellobiose pH 5.0, 95°C, mutant enzyme Q150W Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.B28 57000
-
 x * 57000, SDS-PAGE Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B28 Pyrococcus furiosus Q8U3U9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.B28
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B28 2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Pyrococcus furiosus 2-nitrophenol + D-glucopyranose
-
 ?
3.2.1.B28 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.B28 4-nitrophenyl beta-D-mannopyranoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + beta-D-mannopyranose
-
 ?
3.2.1.B28 cellobiose + H2O
-
 Pyrococcus furiosus 2 beta-D-glucose
-
 ?
3.2.1.B28 lactose + H2O
-
 Pyrococcus furiosus D-glucose + D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.B28 ? x * 57000, SDS-PAGE Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.B28 PFTG
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B28 95
-
 assay at Pyrococcus furiosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.B28 0.03
-
 lactose pH 5.0, 95°C, mutant enzyme Q150W Pyrococcus furiosus
3.2.1.B28 0.03
-
 lactose pH 5.0, 95°C, mutant enzyme Q77R/Q150W Pyrococcus furiosus
3.2.1.B28 0.05
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 95°C, mutant enzyme Q77R/Q150W Pyrococcus furiosus
3.2.1.B28 0.1
-
 cellobiose pH 5.0, 95°C, mutant enzyme Q77R/Q150W Pyrococcus furiosus
3.2.1.B28 0.11
-
 4-nitrophenyl beta-D-mannopyranoside pH 5.0, 95°C, mutant enzyme Q77R/Q150W Pyrococcus furiosus
3.2.1.B28 0.13
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 95°C, mutant enzyme Q150W Pyrococcus furiosus
3.2.1.B28 0.13
-
 4-nitrophenyl beta-D-mannopyranoside pH 5.0, 95°C, mutant enzyme Q150W Pyrococcus furiosus
3.2.1.B28 0.22
-
 2-nitrophenyl beta-D-glucopyranoside pH 5.0, 95°C, mutant enzyme Q77R/Q150W Pyrococcus furiosus
3.2.1.B28 0.67
-
 2-nitrophenyl beta-D-glucopyranoside pH 5.0, 95°C, mutant enzyme Q150W Pyrococcus furiosus
3.2.1.B28 0.74
-
 cellobiose pH 5.0, 95°C, mutant enzyme Q150W Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B28 5
-
 sodium citrate buffer, assay at Pyrococcus furiosus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.B28 1.36
-
 lactose pH 5.0, 95°C, mutant enzyme Q150W Pyrococcus furiosus
3.2.1.B28 1.92
-
 cellobiose pH 5.0, 95°C, mutant enzyme Q150W Pyrococcus furiosus
3.2.1.B28 2.17
-
 2-nitrophenyl beta-D-glucopyranoside pH 5.0, 95°C, mutant enzyme Q77R/Q150W Pyrococcus furiosus
3.2.1.B28 2.21
-
 lactose pH 5.0, 95°C, mutant enzyme Q77R/Q150W Pyrococcus furiosus
3.2.1.B28 2.26
-
 2-nitrophenyl beta-D-glucopyranoside pH 5.0, 95°C, mutant enzyme Q150W Pyrococcus furiosus
3.2.1.B28 2.44
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 95°C, mutant enzyme Q150W Pyrococcus furiosus
3.2.1.B28 2.71
-
 cellobiose pH 5.0, 95°C, mutant enzyme Q77R/Q150W Pyrococcus furiosus
3.2.1.B28 2.83
-
 4-nitrophenyl beta-D-mannopyranoside pH 5.0, 95°C, mutant enzyme Q150W Pyrococcus furiosus
3.2.1.B28 2.95
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 95°C, mutant enzyme Q77R/Q150W Pyrococcus furiosus
3.2.1.B28 3.63
-
 4-nitrophenyl beta-D-mannopyranoside pH 5.0, 95°C, mutant enzyme Q77R/Q150W Pyrococcus furiosus