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Literature summary extracted from

  • Arana, M.; Powell, G.; Edwards, L.; Kunkel, T.; Petrovich, R.
    Refolding active human DNA polymerase ny from inclusion bodies (2010), Protein Expr. Purif., 70, 163-171.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.7 gene POLN, expression of N-terminally His6-tagged enzyme fragment Pol ny-77 in Escherichia coli in inclusion bodies Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.7 Mg2+ required Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.7 77100
-
 N-terminally His6-tagged enzyme fragment Pol ny-77, gel filtration Homo sapiens
2.7.7.7 100000
-
 full-length Pol ny Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn Homo sapiens
-
 diphosphate + DNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Homo sapiens
-
 gene POLN
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.7 recombinant refolded N-terminally His6-tagged enzyme fragment Pol ny-77 by gel filtration, dialysis, and heparin affinity chromatography, recombinant soluble His6-tagged enzyme fragment Pol ny-77 from Escherichia coli by nickel affinity chrmatography Homo sapiens

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.7.7.7 use of high hydrostatic pressure to refold recombinant N-terminally His6-tagged enzyme fragment Pol ny-77 from inclusion bodies after expression in Escherichia coli, in soluble and active form. The refolded Pol ny fragment has properties comparable to those of the small amount of Pol m that is purified from the soluble fraction, method evaluation Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 dATP + DNAn with activated calf thymus DNA Homo sapiens diphosphate + DNAn+1
-
 ?
2.7.7.7 dCTP + DNAn with activated calf thymus DNA Homo sapiens diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Homo sapiens diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn reversibility of the polymerase reaction at the level of the template with diphosphate as leaving group, not with the alternative leaving groups, overview Homo sapiens diphosphate + DNAn+1
-
 ?
2.7.7.7 dGTP + DNAn with activated calf thymus DNA Homo sapiens diphosphate + DNAn+1
-
 ?
2.7.7.7 dTTP + DNAn with activated calf thymus DNA Homo sapiens diphosphate + DNAn+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.7 DNA polymerase ny
-
 Homo sapiens
2.7.7.7 Pol ny
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.7 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.7 8
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
2.7.7.7 evolution DNA polymerase ny is a conserved family A DNA polymerase Homo sapiens