EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.32 | cloning and genetic analysis of fruit-specific phytoene synthase, PSY1, and mutant lines tangerine and yellow-flesh with mutant alleles t3183 and r2997, respectively | Solanum lycopersicum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.32 | G520R | naturally occuring mutation in allele t3406 | Solanum lycopersicum |
2.5.1.32 | G546E | naturally occuring mutation in allele t9776 | Solanum lycopersicum |
2.5.1.32 | L241K | naturally occuring mutation in allele t4838 | Solanum lycopersicum |
2.5.1.32 | additional information | a frameshift mutation due to a single-nucleotide insertion in codon 226 of CRTISO, which leads to an early stop codon, was found in t3002. The other alleles in the M82 variety carry missense mutations: L241K in t4838, G520R in t3406, and G546E in t9776. Expression analysis of wild-type and mutant enzymes in vivo and comparisons, overview | Solanum lycopersicum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.32 | geranylgeranyl diphosphate | Solanum lycopersicum | - |
15-cis-phytoene + 2 diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.32 | Solanum lycopersicum | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.5.1.32 | fruit | - |
Solanum lycopersicum | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.32 | geranylgeranyl diphosphate | - |
Solanum lycopersicum | 15-cis-phytoene + 2 diphosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.32 | fruit-specific phytoene synthase | - |
Solanum lycopersicum |
2.5.1.32 | phytoene synthase 1 | - |
Solanum lycopersicum |
2.5.1.32 | PSY1 | - |
Solanum lycopersicum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.5.1.32 | malfunction | the recessive mutation yellow-flesh (locus r) in tomato eliminates fruit carotenoids by disrupting the activity of the fruit-specific phytoene synthase, PSY1, allele r2997 of yellow-flesh eliminates transcription of PSY1 in fruits. Fruits of the recessive mutation tangerine (t) appear orange due to accumulation of 7,9,7',9'-tetra-cis-lycopene (prolycopene) as a result of a mutation in the carotenoid cis-trans isomerase, tangerine is epistatic to yellow-flesh, interaction between tangerine and yellow-flesh at the molecular level, overview | Solanum lycopersicum |
2.5.1.32 | metabolism | PSY1 catalyzes the first committed step in the carotenoid biosynthesis pathway, the formation of phytoene from two molecules of geranylgeranyl diphosphate | Solanum lycopersicum |
2.5.1.32 | physiological function | molecular mechanism underlying the epistasis of mutations tangerine over yellow-flesh and suggest the involvement of cis-carotenoid metabolites in a feedback regulation of PSY1 gene expression | Solanum lycopersicum |