EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.11.1 | expression in Escherichia coli | Pyrococcus furiosus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.11.1 | Mg2+ | dependent upon a divalent cation, and Mg2+ is the most preferable | Pyrococcus furiosus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.11.1 | 25596 | - |
3 * 25596, calculated from sequence | Pyrococcus furiosus |
3.1.11.1 | 73400 | - |
gel filtration | Pyrococcus furiosus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.11.1 | Pyrococcus furiosus | Q8TZE9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.11.1 | - |
Pyrococcus furiosus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.11.1 | additional information | no endonuclease activity with either ssDNA or dsDNA | Pyrococcus furiosus | ? | - |
? | |
3.1.11.1 | single-stranded oligodeoxyribonucleotide + H2O | excises the DNA at every two nucleotides from the 3' to 5' direction. The enzyme needs to grasp a 46 nt-long DNA strand to express its nucleolytic activity. The homo dT 30 nt-long homooligomer is the most preferable substrate for PfuExo I | Pyrococcus furiosus | single-stranded oligodeoxyribonucleotide + nucleoside 5'-monophosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.11.1 | trimer | 3 * 25596, calculated from sequence | Pyrococcus furiosus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.11.1 | PF2046 | - |
Pyrococcus furiosus |
3.1.11.1 | PfuExo I | - |
Pyrococcus furiosus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.11.1 | 8.8 | - |
assay at | Pyrococcus furiosus |