Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Song, A.A.; Abdullah, J.O.; Abdullah, M.P.; Shafee, N.; Othman, R.; Tan, E.F.; Noor, N.M.; Raha, A.R.
    Overexpressing 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) in the lactococcal mevalonate pathway for heterologous plant sesquiterpene production (2012), PLoS ONE, 7, e52444.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.1.1.34 molecular biology Lactococcus lactis is a potential heterologous host for the production of sesquiterpenes from a herbaceous Malaysian plant, Persicaria minor. A sesquiterpene synthase gene encoding beta-sesquiphellandrene synthase from Persicaria minor is successfully cloned and expressed in Lactococcus lactis. Overexpression of the Lactococcus lactis endogenous 3-hydroxy-3-methylglutaryl Co-A reductase, an established rate-limiting enzyme in the eukaryotic mevalonate pathway, increases the production level of beta-sesquiphellandrene by 1.25-1.60 fold Lactococcus lactis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.34 overexpression of the recombinant His-tagged enzyme in Lactococcus lactis strain NZ9000, co-expression with sesquiterpene synthase gene, PMSTS from Persicaria minor incorporated into the pNZ8048 plasmid, with two base substitutions, 796A.G and 1077A.G, introduced into the PMSTS gene during amplification resulting in exchange K266E and a silent mutation Lactococcus lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.34 (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ Lactococcus lactis
-
 (R)-mevalonate + CoA + 2 NADP+
-
 ?
1.1.1.34 (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ Lactococcus lactis NZ9000
-
 (R)-mevalonate + CoA + 2 NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.34 Lactococcus lactis
-
-
-
1.1.1.34 Lactococcus lactis NZ9000
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.34 (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+
-
 Lactococcus lactis (R)-mevalonate + CoA + 2 NADP+
-
 ?
1.1.1.34 (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+
-
 Lactococcus lactis NZ9000 (R)-mevalonate + CoA + 2 NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.34 3-hydroxy-3-methylglutaryl coenzyme A reductase
-
 Lactococcus lactis
1.1.1.34 HMGR
-
 Lactococcus lactis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.34 30
-
 assay at Lactococcus lactis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.34 7.5
-
 assay at Lactococcus lactis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.34 NADPH
-
 Lactococcus lactis

General Information

EC Number General Information Comment Organism
1.1.1.34 metabolism 3-hydroxy-3-methylglutaryl Co-A reductase is a rate-limiting enzyme in the eukaryotic mevalonate pathway Lactococcus lactis