EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.6.1 | expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.6.1 | ADP | - |
Mycobacterium tuberculosis | |
2.7.6.1 | D-ribose 5-phosphate | - |
Mycobacterium tuberculosis | |
2.7.6.1 | GDP | - |
Mycobacterium tuberculosis | |
2.7.6.1 | UMP | - |
Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.6.1 | 0.014 | - |
D-ribose 5-phosphate | cosubstrate ATP, in 50 mM Tris-HCl pH 8.0, at 25°C | Mycobacterium tuberculosis | |
2.7.6.1 | 0.025 | - |
ATP | in 50 mM Tris-HCl pH 8.0, at 25°C | Mycobacterium tuberculosis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.6.1 | Mg2+ | required | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.6.1 | Mycobacterium tuberculosis | - |
- |
- |
2.7.6.1 | Mycobacterium tuberculosis Rv1017c | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.6.1 | ammonium sulfate precipitation, Q-Sepharose column chromatography, Superdex 200 gel filtration, and Mono Q column chromatography | Mycobacterium tuberculosis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.6.1 | 0.028 | - |
crude extract, in 50 mM Tris-HCl pH 8.0, at 25°C | Mycobacterium tuberculosis |
2.7.6.1 | 1.16 | - |
after 41.4fold purification, in 50 mM Tris-HCl pH 8.0, at 25°C | Mycobacterium tuberculosis |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.6.1 | -80°C, 50 mM Tris-HCl (pH 7.5), up to 7 months, no loss of activity | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.6.1 | ATP + D-ribose 5-phosphate | - |
Mycobacterium tuberculosis | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
2.7.6.1 | ATP + D-ribose 5-phosphate | - |
Mycobacterium tuberculosis Rv1017c | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
2.7.6.1 | CTP + D-ribose 5-phosphate | - |
Mycobacterium tuberculosis | CMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
2.7.6.1 | CTP + D-ribose 5-phosphate | - |
Mycobacterium tuberculosis Rv1017c | CMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
2.7.6.1 | GTP + D-ribose 5-phosphate | - |
Mycobacterium tuberculosis | GMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
2.7.6.1 | GTP + D-ribose 5-phosphate | - |
Mycobacterium tuberculosis Rv1017c | GMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
2.7.6.1 | UTP + D-ribose 5-phosphate | - |
Mycobacterium tuberculosis | UMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
2.7.6.1 | UTP + D-ribose 5-phosphate | - |
Mycobacterium tuberculosis Rv1017c | UMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.6.1 | hexamer | the enzyme predominates as a hexamer, glutaraldehyde cross-linking | Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.6.1 | phosphoribosylpyrophosphate synthase | - |
Mycobacterium tuberculosis |
2.7.6.1 | PRS | - |
Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.6.1 | 0.66 | - |
ATP | in 50 mM Tris-HCl pH 8.0, at 25°C | Mycobacterium tuberculosis | |
2.7.6.1 | 60.68 | - |
D-ribose 5-phosphate | cosubstrate ATP, in 50 mM Tris-HCl pH 8.0, at 25°C | Mycobacterium tuberculosis |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.6.1 | 0.211 | - |
D-ribose 5-phosphate | cosubstrate ATP, in 50 mM Tris-HCl pH 8.0, at 25°C | Mycobacterium tuberculosis |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
2.7.6.1 | 0.07 | - |
in 50 mM Tris-HCl pH 8.0, at 25°C | Mycobacterium tuberculosis | ADP | |
2.7.6.1 | 0.9 | - |
in 50 mM Tris-HCl pH 8.0, at 25°C | Mycobacterium tuberculosis | GDP | |
2.7.6.1 | 3 | - |
in 50 mM Tris-HCl pH 8.0, at 25°C | Mycobacterium tuberculosis | UMP |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.6.1 | 26 | - |
ATP | in 50 mM Tris-HCl pH 8.0, at 25°C | Mycobacterium tuberculosis | |
2.7.6.1 | 59 | - |
D-ribose 5-phosphate | cosubstrate ATP, in 50 mM Tris-HCl pH 8.0, at 25°C | Mycobacterium tuberculosis |