Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Breda, A.; Martinelli, L.K.; Bizarro, C.V.; Rosado, L.A.; Borges, C.B.; Santos, D.S.; Basso, L.A.
    Wild-type phosphoribosylpyrophosphate synthase (PRS) from Mycobacterium tuberculosis: a bacterial class II PRS? (2012), PLoS ONE, 7, e39245.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.6.1 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.6.1 ADP
-
Mycobacterium tuberculosis
2.7.6.1 D-ribose 5-phosphate
-
Mycobacterium tuberculosis
2.7.6.1 GDP
-
Mycobacterium tuberculosis
2.7.6.1 UMP
-
Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.6.1 0.014
-
D-ribose 5-phosphate cosubstrate ATP, in 50 mM Tris-HCl pH 8.0, at 25°C Mycobacterium tuberculosis
2.7.6.1 0.025
-
ATP in 50 mM Tris-HCl pH 8.0, at 25°C Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.6.1 Mg2+ required Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.1 Mycobacterium tuberculosis
-
-
-
2.7.6.1 Mycobacterium tuberculosis Rv1017c
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.6.1 ammonium sulfate precipitation, Q-Sepharose column chromatography, Superdex 200 gel filtration, and Mono Q column chromatography Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.6.1 0.028
-
crude extract, in 50 mM Tris-HCl pH 8.0, at 25°C Mycobacterium tuberculosis
2.7.6.1 1.16
-
after 41.4fold purification, in 50 mM Tris-HCl pH 8.0, at 25°C Mycobacterium tuberculosis

Storage Stability

EC Number Storage Stability Organism
2.7.6.1 -80°C, 50 mM Tris-HCl (pH 7.5), up to 7 months, no loss of activity Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate
-
Mycobacterium tuberculosis AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
?
2.7.6.1 ATP + D-ribose 5-phosphate
-
Mycobacterium tuberculosis Rv1017c AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
?
2.7.6.1 CTP + D-ribose 5-phosphate
-
Mycobacterium tuberculosis CMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
?
2.7.6.1 CTP + D-ribose 5-phosphate
-
Mycobacterium tuberculosis Rv1017c CMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
?
2.7.6.1 GTP + D-ribose 5-phosphate
-
Mycobacterium tuberculosis GMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
?
2.7.6.1 GTP + D-ribose 5-phosphate
-
Mycobacterium tuberculosis Rv1017c GMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
?
2.7.6.1 UTP + D-ribose 5-phosphate
-
Mycobacterium tuberculosis UMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
?
2.7.6.1 UTP + D-ribose 5-phosphate
-
Mycobacterium tuberculosis Rv1017c UMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.7.6.1 hexamer the enzyme predominates as a hexamer, glutaraldehyde cross-linking Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.7.6.1 phosphoribosylpyrophosphate synthase
-
Mycobacterium tuberculosis
2.7.6.1 PRS
-
Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.6.1 0.66
-
ATP in 50 mM Tris-HCl pH 8.0, at 25°C Mycobacterium tuberculosis
2.7.6.1 60.68
-
D-ribose 5-phosphate cosubstrate ATP, in 50 mM Tris-HCl pH 8.0, at 25°C Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.6.1 0.211
-
D-ribose 5-phosphate cosubstrate ATP, in 50 mM Tris-HCl pH 8.0, at 25°C Mycobacterium tuberculosis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.6.1 0.07
-
in 50 mM Tris-HCl pH 8.0, at 25°C Mycobacterium tuberculosis ADP
2.7.6.1 0.9
-
in 50 mM Tris-HCl pH 8.0, at 25°C Mycobacterium tuberculosis GDP
2.7.6.1 3
-
in 50 mM Tris-HCl pH 8.0, at 25°C Mycobacterium tuberculosis UMP

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.6.1 26
-
ATP in 50 mM Tris-HCl pH 8.0, at 25°C Mycobacterium tuberculosis
2.7.6.1 59
-
D-ribose 5-phosphate cosubstrate ATP, in 50 mM Tris-HCl pH 8.0, at 25°C Mycobacterium tuberculosis