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Literature summary extracted from

  • Nobre, A.; Empadinhas, N.; Nobre, M.; Lourenco, E.; Maycock, C.; Ventura, M.; Mingote, A.; da Costa, M.
    The plant Selaginella moellendorffii possesses enzymes for synthesis and hydrolysis of the compatible solutes mannosylglycerate and glucosylglycerate (2012), Planta, 237, 891-901.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.269 expression in Escherichia coli Selaginella moellendorffii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.269 Ca2+ divalent cation inhibit in decreasing order: Sr2+, Ni2, Co2+, Ca2+, Mn2+, Zn2+ Selaginella moellendorffii
2.4.1.269 Co2+ divalent cation inhibit in decreasing order: Sr2+, Ni2, Co2+, Ca2+, Mn2+, Zn2+ Selaginella moellendorffii
2.4.1.269 Mn2+ divalent cation inhibit in decreasing order: Sr2+, Ni2, Co2+, Ca2+, Mn2+, Zn2+ Selaginella moellendorffii
2.4.1.269 Ni2+ divalent cation inhibit in decreasing order: Sr2+, Ni2, Co2+, Ca2+, Mn2+, Zn2+ Selaginella moellendorffii
2.4.1.269 Sr2+ divalent cation inhibit in decreasing order: Sr2+, Ni2, Co2+, Ca2+, Mn2+, Zn2+ Selaginella moellendorffii
2.4.1.269 Zn2+ divalent cation inhibit in decreasing order: Sr2+, Ni2, Co2+, Ca2+, Mn2+, Zn2+ Selaginella moellendorffii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.269 0.2
-
 GDP-mannose pH 8.0, 25°C Selaginella moellendorffii
2.4.1.269 0.6
-
 GDP-mannose pH 8.0, 25°C Selaginella moellendorffii
2.4.1.269 1
-
 GDP-mannose pH 8.0, 50°C Selaginella moellendorffii
2.4.1.269 1.2
-
 D-glycerate cosubstrate GDP-mannose, pH 8.0, 25°C Selaginella moellendorffii
2.4.1.269 1.6
-
 D-glycerate cosubstrate GDP-glucose, pH 8.0, 25°C Selaginella moellendorffii
2.4.1.269 2.4
-
 GDP-mannose pH 8.0, 50°C Selaginella moellendorffii
2.4.1.269 3.4
-
 D-glycerate cosubstrate GDP-glucose, pH 8.0, 50°C Selaginella moellendorffii
2.4.1.269 4.4
-
 D-glycerate cosubstrate GDP-mannose, pH 8.0, 50°C Selaginella moellendorffii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.269 Mg2+ maximum activity at 5 mM Selaginella moellendorffii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.269 47900
-
 4 * 47900, calculated Selaginella moellendorffii
2.4.1.269 217200
-
 gel filtration Selaginella moellendorffii

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.269 Selaginella moellendorffii
-
-
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.269 0°C, stable for more than 2 months Selaginella moellendorffii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.269 GDP-alpha-D-glucose + D-glycerate
-
 Selaginella moellendorffii 2-O-(alpha-D-glucopyranosyl)-D-glycerate + GDP
-
 ?
2.4.1.269 GDP-mannose + D-glycerate
-
 Selaginella moellendorffii 2-O-(alpha-D-mannopyranosyl)-D-glycerate + GDP
-
 ?
2.4.1.269 additional information enzyme is able to efficiently synthesize mannosylglycerate and glucosylglycerate alike Selaginella moellendorffii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.269 tetramer 4 * 47900, calculated Selaginella moellendorffii

Synonyms

EC Number Synonyms Comment Organism
2.4.1.269 MGS
-
 Selaginella moellendorffii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.269 50
-
-
 Selaginella moellendorffii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.269 8
-
-
 Selaginella moellendorffii