BRENDA - Enzyme Database

Catalytic and structural diversity of the fluazifop-inducible glutathione transferases from Phaseolus vulgaris

Chronopoulou, E.; Madesis, P.; Asimakopoulou, B.; Platis, D.; Tsaftaris, A.; Labrou, N.E.; Planta 235, 1253-1269 (2012)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5.1.18
additional information
-
additional information
Michaelis-Menten steady-state kinetics
Phaseolus vulgaris
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.5.1.18
Phaseolus vulgaris
-
isozymes PvGSTF1-1, PvGSTU2-2 and PvGSTU3-3
-
Purification (Commentary)
EC Number
Commentary
Organism
2.5.1.18
native fluazifop-p-butyl-inducible GSTs PvGSTF1-1, PvGSTU2-2 and PvGSTU3-3 from leaves by glutathione affinity chromatography and dialysis
Phaseolus vulgaris
Reaction
EC Number
Reaction
Commentary
Organism
2.5.1.18
RX + glutathione = HX + R-S-glutathione
substrate specificity and catalytic mechanism, overview
Phaseolus vulgaris
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.5.1.18
leaf
-
Phaseolus vulgaris
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.5.1.18
(2,3-dichloro-4-[2-methylenebutyryl]phenoxy)acetic acid + glutathione
i.e. ethacrynic acid
723499
Phaseolus vulgaris
?
-
-
-
?
2.5.1.18
1-bromo-2,4-dinitrobenzene + glutathione
-
723499
Phaseolus vulgaris
S-(2,4-dinitrophenyl)glutathione + HBr
-
-
-
?
2.5.1.18
1-chloro-2,4-dinitrobenzene + glutathione
-
723499
Phaseolus vulgaris
S-(2,4-dinitrophenyl)glutathione + HCl
-
-
-
?
2.5.1.18
1-fluoro-2,4-dinitrobenzene + glutathione
-
723499
Phaseolus vulgaris
S-(2,4-dinitrophenyl)glutathione + HF
-
-
-
?
2.5.1.18
1-iodo-2,4-dinitrobenzene + glutathione
-
723499
Phaseolus vulgaris
S-(2,4-dinitrophenyl)glutathione + HI
-
-
-
?
2.5.1.18
2-hydroxyethyl disulfide + glutathione
-
723499
Phaseolus vulgaris
?
-
-
-
?
2.5.1.18
4-chloro-7-nitrobenzofurazan + glutathione
-
723499
Phaseolus vulgaris
?
-
-
-
?
2.5.1.18
4-nitrobenzyl chloride + glutathione
-
723499
Phaseolus vulgaris
S-(4-nitrophenyl)glutathione + HCl
-
-
-
?
2.5.1.18
alachlor + glutathione
-
723499
Phaseolus vulgaris
?
-
-
-
?
2.5.1.18
allyl isothiocyanate + glutathione
-
723499
Phaseolus vulgaris
?
-
-
-
?
2.5.1.18
atrazine + glutathione
-
723499
Phaseolus vulgaris
?
-
-
-
?
2.5.1.18
bromosulfophthalein + glutathione
-
723499
Phaseolus vulgaris
?
-
-
-
?
2.5.1.18
dehydroascorbate + glutathione
-
723499
Phaseolus vulgaris
?
-
-
-
?
2.5.1.18
fluazifop-p-butyl + glutathione
-
723499
Phaseolus vulgaris
?
-
-
-
?
2.5.1.18
fluorodifen + glutathione
-
723499
Phaseolus vulgaris
?
-
-
-
?
2.5.1.18
metolachlor + glutathione
-
723499
Phaseolus vulgaris
?
-
-
-
?
2.5.1.18
additional information
fluazifop-inducible glutathione transferase isozymes catalyze a broad range of reactions and exhibit quite varied substrate specificity, molecular modeling and structural analysis reveal key structural characteristics, isozynme substrate specificities and catalytic mechanism, overview. Only PvGSTU2-2 shows appreciable activity towards trans-2-nonenal, but the isozymes are also active with cumene hydroperoxide, tert-butyl hydroperoxide, and lauroyl peroxide, no activity of all isozymes with benzoyl peroxide
723499
Phaseolus vulgaris
?
-
-
-
-
2.5.1.18
phenethyl isothiocyanate + glutathione
-
723499
Phaseolus vulgaris
?
-
-
-
?
2.5.1.18
trans-2-nonenal + glutathione
-
723499
Phaseolus vulgaris
?
-
-
-
?
2.5.1.18
trans-4-phenyl-3-buten-2-one + glutathione
-
723499
Phaseolus vulgaris
?
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.5.1.18
37
-
assay at
Phaseolus vulgaris
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5.1.18
7
-
assay at
Phaseolus vulgaris
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5.1.18
additional information
-
additional information
Michaelis-Menten steady-state kinetics
Phaseolus vulgaris
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.5.1.18
native fluazifop-p-butyl-inducible GSTs PvGSTF1-1, PvGSTU2-2 and PvGSTU3-3 from leaves by glutathione affinity chromatography and dialysis
Phaseolus vulgaris
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.5.1.18
leaf
-
Phaseolus vulgaris
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.5.1.18
(2,3-dichloro-4-[2-methylenebutyryl]phenoxy)acetic acid + glutathione
i.e. ethacrynic acid
723499
Phaseolus vulgaris
?
-
-
-
?
2.5.1.18
1-bromo-2,4-dinitrobenzene + glutathione
-
723499
Phaseolus vulgaris
S-(2,4-dinitrophenyl)glutathione + HBr
-
-
-
?
2.5.1.18
1-chloro-2,4-dinitrobenzene + glutathione
-
723499
Phaseolus vulgaris
S-(2,4-dinitrophenyl)glutathione + HCl
-
-
-
?
2.5.1.18
1-fluoro-2,4-dinitrobenzene + glutathione
-
723499
Phaseolus vulgaris
S-(2,4-dinitrophenyl)glutathione + HF
-
-
-
?
2.5.1.18
1-iodo-2,4-dinitrobenzene + glutathione
-
723499
Phaseolus vulgaris
S-(2,4-dinitrophenyl)glutathione + HI
-
-
-
?
2.5.1.18
2-hydroxyethyl disulfide + glutathione
-
723499
Phaseolus vulgaris
?
-
-
-
?
2.5.1.18
4-chloro-7-nitrobenzofurazan + glutathione
-
723499
Phaseolus vulgaris
?
-
-
-
?
2.5.1.18
4-nitrobenzyl chloride + glutathione
-
723499
Phaseolus vulgaris
S-(4-nitrophenyl)glutathione + HCl
-
-
-
?
2.5.1.18
alachlor + glutathione
-
723499
Phaseolus vulgaris
?
-
-
-
?
2.5.1.18
allyl isothiocyanate + glutathione
-
723499
Phaseolus vulgaris
?
-
-
-
?
2.5.1.18
atrazine + glutathione
-
723499
Phaseolus vulgaris
?
-
-
-
?
2.5.1.18
bromosulfophthalein + glutathione
-
723499
Phaseolus vulgaris
?
-
-
-
?
2.5.1.18
dehydroascorbate + glutathione
-
723499
Phaseolus vulgaris
?
-
-
-
?
2.5.1.18
fluazifop-p-butyl + glutathione
-
723499
Phaseolus vulgaris
?
-
-
-
?
2.5.1.18
fluorodifen + glutathione
-
723499
Phaseolus vulgaris
?
-
-
-
?
2.5.1.18
metolachlor + glutathione
-
723499
Phaseolus vulgaris
?
-
-
-
?
2.5.1.18
additional information
fluazifop-inducible glutathione transferase isozymes catalyze a broad range of reactions and exhibit quite varied substrate specificity, molecular modeling and structural analysis reveal key structural characteristics, isozynme substrate specificities and catalytic mechanism, overview. Only PvGSTU2-2 shows appreciable activity towards trans-2-nonenal, but the isozymes are also active with cumene hydroperoxide, tert-butyl hydroperoxide, and lauroyl peroxide, no activity of all isozymes with benzoyl peroxide
723499
Phaseolus vulgaris
?
-
-
-
-
2.5.1.18
phenethyl isothiocyanate + glutathione
-
723499
Phaseolus vulgaris
?
-
-
-
?
2.5.1.18
trans-2-nonenal + glutathione
-
723499
Phaseolus vulgaris
?
-
-
-
?
2.5.1.18
trans-4-phenyl-3-buten-2-one + glutathione
-
723499
Phaseolus vulgaris
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.5.1.18
37
-
assay at
Phaseolus vulgaris
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.5.1.18
7
-
assay at
Phaseolus vulgaris
Expression
EC Number
Organism
Commentary
Expression
2.5.1.18
Phaseolus vulgaris
treatment of Phaseolus vulgaris leaves with the aryloxyphenoxypropionic herbicide fluazifop-p-butyl, i.e. butyl 2-[4-[[5-(trifluoromethyl)-2-pyridinyl]oxy]phenoxy]propanoate, results in induction of three GST isozyme activities, i.e. PvGSTF1-1, PvGSTU2-2 and PvGSTU3-3
up
Expression (protein specific)
EC Number
Organism
Commentary
Expression
2.5.1.18
Phaseolus vulgaris
treatment of Phaseolus vulgaris leaves with the aryloxyphenoxypropionic herbicide fluazifop-p-butyl, i.e. butyl 2-[4-[[5-(trifluoromethyl)-2-pyridinyl]oxy]phenoxy]propanoate, results in induction of three GST isozyme activities, i.e. PvGSTF1-1, PvGSTU2-2 and PvGSTU3-3
up