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Literature summary extracted from

  • Bailey, B.A.; Larson, R.L.
    Maize microsomal benzoxazinone N-monooxygenase (1991), Plant Physiol., 95, 792-796.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.14.110 EDTA 49% enhancement of activity at 5 mM Zea mays
1.14.14.110 additional information not affected by 5 mM diethyldithiocarbamate Zea mays

General Stability

EC Number General Stability Organism
1.14.14.110 extraction of microsomes in the absence of dithioerythritol results in total loss of activity, but exclusion of dithioerythritol from the assay buffer has no effect on enzyme activity Zea mays

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.110 2,2'-dipyridyl 5 mM inhibits activity by 57% Zea mays
1.14.14.110 Ca2+ 5 mM Ca2+ inhibits activity by 69% Zea mays
1.14.14.110 carbon monoxide the effect of carbon monoxide can be reversed by exposing the enzyme to 450 nanometer light during the incubation period Zea mays
1.14.14.110 cytochrome c
-
Zea mays
1.14.14.110 Fe2+ 5 mM Fe2+ inhibits activity by 64% Zea mays
1.14.14.110 Hg2+ complete inhibition at 5 mM Zea mays
1.14.14.110 Mg2+ 5 mM Mg2+ inhibits activity by 45% Zea mays
1.14.14.110 Mn2+ complete inhibition at 5 mM Zea mays
1.14.14.110 N-ethylmaleimide 5 mM inhibits activity by 97% Zea mays
1.14.14.110 N2 the effect of nitrogen can be reversed by exposing the enzyme to air Zea mays
1.14.14.110 NADP+
-
Zea mays
1.14.14.110 NaN3 5 mM NaN3 inhibits activity by 16% Zea mays
1.14.14.110 p-chloromercuribenzoate 5 mM inhibits activity by 58% Zea mays
1.14.14.110 Zn2+ complete inhibition at 5 mM Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.110 0.005
-
NADPH in 50 mM HEPES, pH 8.5, at 35°C Zea mays
1.14.14.110 0.013
-
2-hydroxy-2H-1,4-benzoxazin-3(4H)-one in 50 mM HEPES, pH 8.5, at 35°C Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.110 microsome
-
Zea mays
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.110 Zea mays
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.110 seedling
-
Zea mays
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.110 2-hydroxy-2H-1,4-benzoxazin-3(4H)-one + NADPH + H+ + O2
-
Zea mays 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + NADP+ + H2O
-
r
1.14.14.110 2-hydroxy-2H-1,4-benzoxazin-3(4H)-one + [reduced NADPH-hemoprotein reductase] + O2
-
Zea mays 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.14.110 additional information no activity with NADH and FAD Zea mays ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.110 benzoxazinone N-monooxygenase
-
Zea mays

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.110 35
-
-
Zea mays

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.14.110 100
-
heating the enzyme for 10 min at 100°C in a boiling water bath results in a total loss of activity Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.110 7.5
-
-
Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.110 cytochrome P-450 dependent on Zea mays