EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.110 | EDTA | 49% enhancement of activity at 5 mM | Zea mays | |
1.14.14.110 | additional information | not affected by 5 mM diethyldithiocarbamate | Zea mays |
EC Number | General Stability | Organism |
---|---|---|
1.14.14.110 | extraction of microsomes in the absence of dithioerythritol results in total loss of activity, but exclusion of dithioerythritol from the assay buffer has no effect on enzyme activity | Zea mays |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.110 | 2,2'-dipyridyl | 5 mM inhibits activity by 57% | Zea mays | |
1.14.14.110 | Ca2+ | 5 mM Ca2+ inhibits activity by 69% | Zea mays | |
1.14.14.110 | carbon monoxide | the effect of carbon monoxide can be reversed by exposing the enzyme to 450 nanometer light during the incubation period | Zea mays | |
1.14.14.110 | cytochrome c | - |
Zea mays | |
1.14.14.110 | Fe2+ | 5 mM Fe2+ inhibits activity by 64% | Zea mays | |
1.14.14.110 | Hg2+ | complete inhibition at 5 mM | Zea mays | |
1.14.14.110 | Mg2+ | 5 mM Mg2+ inhibits activity by 45% | Zea mays | |
1.14.14.110 | Mn2+ | complete inhibition at 5 mM | Zea mays | |
1.14.14.110 | N-ethylmaleimide | 5 mM inhibits activity by 97% | Zea mays | |
1.14.14.110 | N2 | the effect of nitrogen can be reversed by exposing the enzyme to air | Zea mays | |
1.14.14.110 | NADP+ | - |
Zea mays | |
1.14.14.110 | NaN3 | 5 mM NaN3 inhibits activity by 16% | Zea mays | |
1.14.14.110 | p-chloromercuribenzoate | 5 mM inhibits activity by 58% | Zea mays | |
1.14.14.110 | Zn2+ | complete inhibition at 5 mM | Zea mays |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.110 | 0.005 | - |
NADPH | in 50 mM HEPES, pH 8.5, at 35°C | Zea mays | |
1.14.14.110 | 0.013 | - |
2-hydroxy-2H-1,4-benzoxazin-3(4H)-one | in 50 mM HEPES, pH 8.5, at 35°C | Zea mays |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.14.110 | microsome | - |
Zea mays | - |
- |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.110 | Zea mays | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.14.110 | seedling | - |
Zea mays | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.110 | 2-hydroxy-2H-1,4-benzoxazin-3(4H)-one + NADPH + H+ + O2 | - |
Zea mays | 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + NADP+ + H2O | - |
r | |
1.14.14.110 | 2-hydroxy-2H-1,4-benzoxazin-3(4H)-one + [reduced NADPH-hemoprotein reductase] + O2 | - |
Zea mays | 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.110 | additional information | no activity with NADH and FAD | Zea mays | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.110 | benzoxazinone N-monooxygenase | - |
Zea mays |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.14.110 | 35 | - |
- |
Zea mays |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.14.110 | 100 | - |
heating the enzyme for 10 min at 100°C in a boiling water bath results in a total loss of activity | Zea mays |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.14.110 | 7.5 | - |
- |
Zea mays |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.110 | cytochrome P-450 | dependent on | Zea mays |