BRENDA - Enzyme Database

Fumarate and cytosolic pH as modulators of the synthesis or consumption of C4 organic acids through NADP-malic enzyme in Arabidopsis thaliana

Arias, C.; Andreo, C.; Drincovich, M.; Gerrard Wheeler, M.; Plant Mol. Biol. 81, 297-307 (2013)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.40
fumarate
fumarate activates the NADP-ME2 forward reaction by about 15fold at 0.05 mM NADP+, but only at about 1.5fold at 0.35 mM NADP+. The trans configuration of fumarate is crucial for the activating effect
Arabidopsis thaliana
1.1.1.40
succinate
activates at 10 mM
Arabidopsis thaliana
Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.40
expression of isozyme NADP-ME2 in Escherichia coli strain BL21(DE3)
Arabidopsis thaliana
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.40
R115A
site-directed mutagenesis of isozyme NADP-ME2, the mutant displays a marked inhibition in the presence of all the organic acids tested, also fumarate
Arabidopsis thaliana
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.40
2-oxoglutarate
inhibition of isozyme NADP-ME2
Arabidopsis thaliana
1.1.1.40
Maleate
the cis isomer of fumarate, inhibition of isozyme NADP-ME2
Arabidopsis thaliana
1.1.1.40
malonate
inhibition of isozyme NADP-ME2
Arabidopsis thaliana
1.1.1.40
additional information
no inhibition of isozyme NADP-ME2 by tartrate
Arabidopsis thaliana
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.40
additional information
-
additional information
kinetics of NADP+ and (S)-malate at different NADP+ and fumarate concentrations, overview
Arabidopsis thaliana
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.40
Mg2+
required
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.40
(S)-malate + NADP+
Arabidopsis thaliana
-
pyruvate + CO2 + NADPH
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.40
Arabidopsis thaliana
-
four malic isozymes, NADP-ME1, NADP-ME2, NADP-ME3, and NADP-ME4
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.40
(S)-malate + NADP+
-
723442
Arabidopsis thaliana
pyruvate + CO2 + NADPH
-
-
-
r
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.40
30
-
assay at
Arabidopsis thaliana
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.40
7
-
reductive carboxylation of pyruvate
Arabidopsis thaliana
1.1.1.40
7.5
-
oxidative decarboxylation of malate
Arabidopsis thaliana
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.40
NADP+
dependent on
Arabidopsis thaliana
1.1.1.40
NADPH
dependent on
Arabidopsis thaliana
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.40
fumarate
fumarate activates the NADP-ME2 forward reaction by about 15fold at 0.05 mM NADP+, but only at about 1.5fold at 0.35 mM NADP+. The trans configuration of fumarate is crucial for the activating effect
Arabidopsis thaliana
1.1.1.40
succinate
activates at 10 mM
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.40
expression of isozyme NADP-ME2 in Escherichia coli strain BL21(DE3)
Arabidopsis thaliana
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.40
NADP+
dependent on
Arabidopsis thaliana
1.1.1.40
NADPH
dependent on
Arabidopsis thaliana
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.40
R115A
site-directed mutagenesis of isozyme NADP-ME2, the mutant displays a marked inhibition in the presence of all the organic acids tested, also fumarate
Arabidopsis thaliana
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.40
2-oxoglutarate
inhibition of isozyme NADP-ME2
Arabidopsis thaliana
1.1.1.40
Maleate
the cis isomer of fumarate, inhibition of isozyme NADP-ME2
Arabidopsis thaliana
1.1.1.40
malonate
inhibition of isozyme NADP-ME2
Arabidopsis thaliana
1.1.1.40
additional information
no inhibition of isozyme NADP-ME2 by tartrate
Arabidopsis thaliana
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.40
additional information
-
additional information
kinetics of NADP+ and (S)-malate at different NADP+ and fumarate concentrations, overview
Arabidopsis thaliana
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.40
Mg2+
required
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.40
(S)-malate + NADP+
Arabidopsis thaliana
-
pyruvate + CO2 + NADPH
-
-
r
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.40
(S)-malate + NADP+
-
723442
Arabidopsis thaliana
pyruvate + CO2 + NADPH
-
-
-
r
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.40
30
-
assay at
Arabidopsis thaliana
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.40
7
-
reductive carboxylation of pyruvate
Arabidopsis thaliana
1.1.1.40
7.5
-
oxidative decarboxylation of malate
Arabidopsis thaliana