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Literature summary extracted from

  • Chen, F.; D'Auria, J.C.; Tholl, D.; Ross, J.R.; Gershenzon, J.; Noel, J.P.; Pichersky, E.
    An Arabidopsis thaliana gene for methylsalicylate biosynthesis, identified by a biochemical genomics approach, has a role in defense (2003), Plant J., 36, 577-588.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.273 alamethicin treatment of detached Arabidopsis thaliana leaves efficiently induced methyl salicylate but not methyl benzoate emission Arabidopsis thaliana
2.1.1.273 insect herbivory feeding by the larvae of the diamondback moth on rosette leaves of intact plants resultes in low methyl salicylate and very low methyl benzoate emission Arabidopsis thaliana
2.1.1.274 alamethicin treatment of detached Arabidopsis thaliana leaves efficiently induced methyl salicylate but not methyl benzoate emission Arabidopsis thaliana
2.1.1.274 insect herbivory feeding by the larvae of the diamondback moth on rosette leaves of intact plants resulted in low methyl salicylate and very low methyl benzoate emission Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.273 coding regions of AlBSMT1 ligated into pCRT7/CT-TOPO TA vector for functional expression in Escherichia coli Arabidopsis lyrata
2.1.1.273 coding regions of AtBSMT1 ligated into pCRT7/CT-TOPO TA vector for functional expression in Escherichia coli Arabidopsis thaliana
2.1.1.274 coding regions of AlBSMT1 ligated into pCRT7/CT-TOPO TA vector for functional expression in Escherichia coli Arabidopsis lyrata
2.1.1.274 coding regions of AtBSMT1 ligated into pCRT7/CT-TOPO TA vector for functional expression in Escherichia coli Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.273 0.065
-
benzoic acid apparent Km-value, pH 7.7, 30°C Arabidopsis thaliana
2.1.1.273 0.067
-
S-adenosyl-L-methionine apparent Km-value, pH 7.7, 30°C Arabidopsis thaliana
2.1.1.273 0.087
-
S-adenosyl-L-methionine apparent Km-value, pH 7.7, 30°C Arabidopsis lyrata
2.1.1.273 0.131
-
benzoic acid apparent Km-value, pH 7.7, 30°C Arabidopsis lyrata
2.1.1.274 0.016
-
salicylic acid apparent Km-value, pH 7.7, 30°C Arabidopsis thaliana
2.1.1.274 0.067
-
S-adenosyl-L-methionine apparent Km-value, pH 7.7, 30°C Arabidopsis thaliana
2.1.1.274 0.087
-
S-adenosyl-L-methionine apparent Km-value, pH 7.7, 30°C Arabidopsis lyrata
2.1.1.274 0.127
-
salicylic acid apparent Km-value, pH 7.7, 30°C Arabidopsis lyrata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.273 43360
-
AlBSMT1 subunit, calculated from sequence Arabidopsis lyrata
2.1.1.273 43363
-
2 * 44000, SDS–PAGE, 2 * 43363 calculated from sequence Arabidopsis lyrata
2.1.1.273 43365
-
2 * 44000, SDS–PAGE, 2 * 43365 calculated from sequence Arabidopsis thaliana
2.1.1.273 43370
-
subunit, calculated from sequence Arabidopsis thaliana
2.1.1.273 44000
-
AlBSMT1 subunit mass by SDS–PAGE Arabidopsis lyrata
2.1.1.273 44000
-
subunit mass by SDS–PAGE Arabidopsis thaliana
2.1.1.273 91000
-
AlBSMT1 holoenzyme, estimated by size exclusion chromatography Arabidopsis lyrata
2.1.1.273 91000
-
holoenzyme, estimated by size exclusion chromatography Arabidopsis thaliana
2.1.1.274 43360
-
AlBSMT1 subunit, calculated from sequence Arabidopsis lyrata
2.1.1.274 43363
-
2 * 44000, by SDS–PAGE, 2 * 43363, calculated from sequence Arabidopsis lyrata
2.1.1.274 43365
-
2 * 44000, by SDS–PAGE, 2 * 43365, calculated from sequence Arabidopsis thaliana
2.1.1.274 43370
-
AtBSMT1 subunit, calculated from sequence Arabidopsis thaliana
2.1.1.274 44000
-
AlBSMT1 subunit mass by SDS–PAGE Arabidopsis lyrata
2.1.1.274 44000
-
AtBSMT1 subunit mass by SDS–PAGE Arabidopsis thaliana
2.1.1.274 91000
-
AlBSMT1 holoenzyme, estimated by size exclusion chromatography Arabidopsis lyrata
2.1.1.274 91000
-
AtBSMT1 holoenzyme, estimated by size exclusion chromatography Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.273 Arabidopsis lyrata Q6XMI1
-
-
2.1.1.273 Arabidopsis thaliana Q6XMI3
-
-
2.1.1.274 Arabidopsis lyrata Q6XMI1
-
-
2.1.1.274 Arabidopsis thaliana Q6XMI3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.273 to near homogeneity in two steps, first using anion-exchange column diethylaminoethyl cellulose followed by Mono-Q anion-exchange chromatography Arabidopsis thaliana
2.1.1.273 to near homogeneity in two steps, first using anion-exchange column diethylaminoethyl cellulose followed by Mono-Q anion-exchange chromatography Arabidopsis lyrata
2.1.1.274 to near homogeneity in two steps, first using anion-exchange column diethylaminoethyl cellulose followed by Mono-Q anion-exchange chromatography Arabidopsis thaliana
2.1.1.274 to near homogeneity in two steps, first using anion-exchange column diethylaminoethyl cellulose followed by Mono-Q anion-exchange chromatography Arabidopsis lyrata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.273 flower AlBSMT1 transcript levels are, similarly to AtBSMT1 transcripts in Arabidopsis thaliana higher in flowers Arabidopsis lyrata
-
2.1.1.273 flower highest levels of AtBSMT1 transcript Arabidopsis thaliana
-
2.1.1.273 leaf detectable AlBSMT1 transcript levels Arabidopsis lyrata
-
2.1.1.273 leaf lower levels of AtBSMT1 transcript Arabidopsis thaliana
-
2.1.1.273 additional information little or no AtBSMT1 transcripts observed in roots or siliques of plants Arabidopsis thaliana
-
2.1.1.273 stem lower levels of AtBSMT1 transcript Arabidopsis thaliana
-
2.1.1.274 flower AlBSMT1 transcript levels are, similarly to AtBSMT1 transcripts in Arabidopsis thaliana higher in flowers Arabidopsis lyrata
-
2.1.1.274 flower highest levels of AtBSMT1 transcript Arabidopsis thaliana
-
2.1.1.274 leaf detectable AlBSMT1 transcript levels Arabidopsis lyrata
-
2.1.1.274 leaf lower levels of AtBSMT1 transcript Arabidopsis thaliana
-
2.1.1.274 additional information little or no AtBSMT1 transcripts observed in roots or siliques of plants Arabidopsis thaliana
-
2.1.1.274 stem lower levels of AtBSMT1 transcript Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.273 additional information in contrast to AtBSMT1 no activity with 1 mM 3-hydroxybenzoic acid Arabidopsis lyrata ?
-
?
2.1.1.273 additional information The enzyme, which is found in flowering plants, also has the activity of EC 2.1.1.274, salicylate 1-O-methyltransferase Arabidopsis thaliana ?
-
?
2.1.1.273 additional information The enzyme, which is found in flowering plants, also has the activity of EC 2.1.1.274, salicylate 1-O-methyltransferase Arabidopsis lyrata ?
-
?
2.1.1.273 S-adenosyl-L-methionine + 3-hydroxybenzoic acid 54% relative activity at 1 mM methyl acceptor compared to activity with benzoic acid set at 100% Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl 3-hydroxybenzoate
-
?
2.1.1.273 S-adenosyl-L-methionine + anthranilic acid 32% relative activity at 1 mM methyl acceptor compared to activity with benzoic acid set at 100% Arabidopsis lyrata S-adenosyl-L-homocysteine + methyl anthranilate
-
?
2.1.1.273 S-adenosyl-L-methionine + anthranilic acid 35% relative activity at 1 mM methyl acceptor compared to activity with benzoic acid set at 100% Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl anthranilate
-
?
2.1.1.273 S-adenosyl-L-methionine + benzoic acid
-
Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl benzoate
-
?
2.1.1.273 S-adenosyl-L-methionine + benzoic acid
-
Arabidopsis lyrata S-adenosyl-L-homocysteine + methyl benzoate
-
?
2.1.1.274 additional information enzyme is also active with benzoic acid resulting in methyl benzoate formation Arabidopsis thaliana ?
-
?
2.1.1.274 additional information enzyme is also active with benzoic acid resulting in methyl benzoate formation Arabidopsis lyrata ?
-
?
2.1.1.274 additional information in contrast to AtBSMT1 no activity with 1 mM 3-hydroxybenzoic acid Arabidopsis lyrata ?
-
?
2.1.1.274 additional information The enzyme, which is found in flowering plants, also has the activity of EC 2.1.1.273, benzoate O-methyltransferase Arabidopsis thaliana ?
-
?
2.1.1.274 additional information The enzyme, which is found in flowering plants, also has the activity of EC 2.1.1.273, benzoate O-methyltransferase Arabidopsis lyrata ?
-
?
2.1.1.274 S-adenosyl-L-methionine + 3-hydroxybenzoic acid 54% relative activity at 1 mM methyl acceptor compared to activity with benzoic acid set at 100% Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl 3-hydroxybenzoate
-
?
2.1.1.274 S-adenosyl-L-methionine + anthranilic acid 32% relative activity at 1 mM methyl acceptor compared to activity with benzoic acid set at 100% Arabidopsis lyrata S-adenosyl-L-homocysteine + methyl anthranilate
-
?
2.1.1.274 S-adenosyl-L-methionine + anthranilic acid 35% relative activity at 1 mM methyl acceptor compared to activity with benzoic acid set at 100% Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl anthranilate
-
?
2.1.1.274 S-adenosyl-L-methionine + salicylic acid 16% relative activity at 1 mM methyl acceptor compared to activity with benzoic acid set at 100% Arabidopsis lyrata S-adenosyl-L-homocysteine + methyl salicylate
-
?
2.1.1.274 S-adenosyl-L-methionine + salicylic acid 46% relative activity at 1 mM methyl acceptor compared to activity with benzoic acid set at 100% Arabidopsis thaliana S-adenosyl-L-homocysteine + methyl salicylate
-
?

Subunits

EC Number Subunits Comment Organism
2.1.1.273 homodimer 2 * 44000, SDS–PAGE, 2 * 43363 calculated from sequence Arabidopsis lyrata
2.1.1.273 homodimer 2 * 44000, SDS–PAGE, 2 * 43365 calculated from sequence Arabidopsis thaliana
2.1.1.274 homodimer 2 * 44000, by SDS–PAGE, 2 * 43363, calculated from sequence Arabidopsis lyrata
2.1.1.274 homodimer 2 * 44000, by SDS–PAGE, 2 * 43365, calculated from sequence Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.1.1.273 AlBSMT1
-
Arabidopsis lyrata
2.1.1.273 AtBSMT1
-
Arabidopsis thaliana
2.1.1.274 AlBSMT1
-
Arabidopsis lyrata
2.1.1.274 AtBSMT1
-
Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.273 0.19
-
benzoic acid pH 7.7, 30°C Arabidopsis thaliana
2.1.1.273 0.3
-
benzoic acid pH 7.7, 30°C Arabidopsis lyrata
2.1.1.274 0.05
-
salicylic acid pH 7.7, 30°C Arabidopsis lyrata
2.1.1.274 0.07
-
salicylic acid pH 7.7, 30°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.273 7 8
-
Arabidopsis thaliana
2.1.1.273 7 8
-
Arabidopsis lyrata
2.1.1.274 7 8
-
Arabidopsis thaliana
2.1.1.274 7 8
-
Arabidopsis lyrata

Expression

EC Number Organism Comment Expression
2.1.1.273 Arabidopsis thaliana under normal growing conditions AtBSMT1 promoter is only active in leaves at the base of the trichomes, in the hydathodes, and in the upper part of the sepals of flowers. Strong AtBSMT1 promoter activity is observed around lesions in the leaves caused by thrips of the genus Frankliniella additional information
2.1.1.273 Arabidopsis thaliana leaf wounding and uprooting causes AtBSMT1 transcripts to accumulate quickly within 2 h after treatment with uprooting being more effective up
2.1.1.273 Arabidopsis thaliana methyl jasmonate treatment, in which plants are exposed to methyl jasmonate vapors in a closed container, results in slow induction up
2.1.1.274 Arabidopsis thaliana under normal growing conditions AtBSMT1 promoter is only active in leaves at the base of the trichomes, in the hydathodes, and in the upper part of the sepals of flowers. Strong AtBSMT1 promoter activity is observed around lesions in the leaves caused by thrips of the genus Frankliniella additional information
2.1.1.274 Arabidopsis thaliana leaf wounding and uprooting causes AtBSMT1 transcripts to accumulate quickly within 2 h after treatment with uprooting being more effective up
2.1.1.274 Arabidopsis thaliana methyl jasmonate treatment, in which plants are exposed to methyl jasmonate vapors in a closed container, results in slow induction up

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.273 2.9
-
benzoic acid pH 7.7, 30°C Arabidopsis thaliana
2.1.1.273 9.6
-
benzoic acid pH 7.7, 30°C Arabidopsis lyrata
2.1.1.274 0.4
-
salicylic acid pH 7.7, 30°C Arabidopsis lyrata
2.1.1.274 4.3
-
salicylic acid pH 7.7, 30°C Arabidopsis thaliana