EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.275 | NH4+ | 5 mM, 4fold activation | Arabidopsis thaliana |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.275 | expression in Escherichia coli, expression in Arabidopsis under the control of the 35S cauliflower mosaic virus promoter | Arabidopsis thaliana |
2.1.1.276 | expression in Escherichia coli, expression in Arabidopsis thaliana under the control of the 35S cauliflower mosaic virus promoter | Arabidopsis thaliana |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.275 | Cu2+ | 5 mM, more than 90% inhibition | Arabidopsis thaliana | |
2.1.1.275 | Fe2+ | 5 mM, more than 90% inhibition | Arabidopsis thaliana | |
2.1.1.275 | Fe3+ | 5 mM, more than 90% inhibition | Arabidopsis thaliana | |
2.1.1.275 | Zn2+ | 5 mM, more than 90% inhibition | Arabidopsis thaliana | |
2.1.1.276 | Cu2+ | 5 mM, more than 90% inhibition | Arabidopsis thaliana | |
2.1.1.276 | Fe3+ | 5 mM, more than 90% inhibition | Arabidopsis thaliana | |
2.1.1.276 | Zn2+ | 5 mM, more than 90% inhibition | Arabidopsis thaliana |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.275 | 0.0054 | - |
gibberellin a4 | pH 8.0, 30°C | Arabidopsis thaliana | |
2.1.1.275 | 0.0158 | - |
gibberellin a9 | pH 8.0, 30°C | Arabidopsis thaliana | |
2.1.1.276 | 0.0019 | - |
gibberellin a9 | pH 8.0, 30°C | Arabidopsis thaliana | |
2.1.1.276 | 0.0059 | - |
gibberellin a4 | pH 8.0, 30°C | Arabidopsis thaliana |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.275 | K+ | 5 mM, 3fold activation | Arabidopsis thaliana | |
2.1.1.276 | additional information | not enhanced nor inhibited by K+, NH4+, Na+, Ca2+, Fe2þ, Mg2+, and Mn2+ | Arabidopsis thaliana |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.275 | 40000 | - |
x * 40000, SDS-PAGE | Arabidopsis thaliana |
2.1.1.275 | 41500 | - |
x * 41500, calculated from sequence | Arabidopsis thaliana |
2.1.1.276 | 43300 | - |
x * 43300, calculated from sequence | Arabidopsis thaliana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.275 | S-adenosyl-L-methionine + gibberellin A9 | Arabidopsis thaliana | methylation of gibberellins by the enzyme serves to deactivate gibberellins and initiate their degradation as the seeds mature | S-adenosyl-L-homocysteine + methyl gibberellin A9 | - |
? | |
2.1.1.276 | S-adenosyl-L-methionine + gibberellin A4 | Arabidopsis thaliana | - |
S-adenosyl-L-homocysteine + methyl gibberellin A4 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.275 | Arabidopsis thaliana | F4JUY5 | - |
- |
2.1.1.276 | Arabidopsis thaliana | Q5XF78 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.275 | - |
Arabidopsis thaliana |
2.1.1.276 | - |
Arabidopsis thaliana |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.1.275 | seed | GAMT1 expression is localized mostly to seed | Arabidopsis thaliana | - |
2.1.1.275 | silique | expressed mainly in whole siliques, peak transcript levels from the middle until the end of silique development | Arabidopsis thaliana | - |
2.1.1.276 | seed | - |
Arabidopsis thaliana | - |
2.1.1.276 | silique | - |
Arabidopsis thaliana | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.275 | S-adenosyl-L-methionine + gibberellin 1 | 25% of the activity compared to methylation of gibberellin A9 | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A1 | - |
? | |
2.1.1.275 | S-adenosyl-L-methionine + gibberellin A19 | 19% of the activity compared to methylation of gibberellin A9 | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A19 | - |
? | |
2.1.1.275 | S-adenosyl-L-methionine + gibberellin A20 | 95% of the activity compared to methylation of gibberellin A9 | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A20 | - |
? | |
2.1.1.275 | S-adenosyl-L-methionine + gibberellin A3 | 80% of the activity compared to methylation of gibberellin A9 | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A3 | - |
? | |
2.1.1.275 | S-adenosyl-L-methionine + gibberellin A34 | 46% of the activity compared to methylation of gibberellin A9 | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A34 | - |
? | |
2.1.1.275 | S-adenosyl-L-methionine + gibberellin A4 | 69% of the activity compared to methylation of gibberellin A9 | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A4 | - |
? | |
2.1.1.275 | S-adenosyl-L-methionine + gibberellin A51 | 32% of the activity compared to methylation of gibberellin A9 | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A51 | - |
? | |
2.1.1.275 | S-adenosyl-L-methionine + gibberellin A9 | methylation of gibberellins by the enzyme serves to deactivate gibberellins and initiate their degradation as the seeds mature | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A9 | - |
? | |
2.1.1.275 | S-adenosyl-L-methionine + gibberellin A9 | gibberellin A9 i.e. (1R,4aR,4bR,7R,9aR,10S,10aR)-1-methyl-8-methylene-13-oxododecahydro-4a,1-(epoxymethano)-7,9a-methanobenzo[a]azulene-10-carboxylic acid | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A9 | methyl gibberellin A9 i.e. methyl (1R,4aR,4bR,7R,9aR,10S,10aR)-1-methyl-8-methylene-13-oxododecahydro-4a,1-(epoxymethano)-7,9a-methanobenzo[a]azulene-10-carboxylate | ? | |
2.1.1.276 | additional information | no activity with gibberelin A12 and gibberelin A19 | Arabidopsis thaliana | ? | - |
? | |
2.1.1.276 | S-adenosyl-L-methionine + gibberellin A1 | 30% of the activity compared to methylation of gibberellin A4 | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A1 | - |
? | |
2.1.1.276 | S-adenosyl-L-methionine + gibberellin A20 | 15% of the activity compared to methylation of gibberellin A4 | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A20 | - |
? | |
2.1.1.276 | S-adenosyl-L-methionine + gibberellin A3 | 45% of the activity compared to methylation of gibberellin A4 | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A3 | - |
? | |
2.1.1.276 | S-adenosyl-L-methionine + gibberellin A34 | 80% of the activity compared to methylation of gibberellin A4 | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A34 | - |
? | |
2.1.1.276 | S-adenosyl-L-methionine + gibberellin A4 | - |
Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A4 | - |
? | |
2.1.1.276 | S-adenosyl-L-methionine + gibberellin A51 | 16% of the activity compared to methylation of gibberellin A4 | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A51 | - |
? | |
2.1.1.276 | S-adenosyl-L-methionine + gibberellin A9 | 60% of the activity compared to methylation of gibberellin A4 | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl gibberellin A9 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.275 | ? | x * 40000, SDS-PAGE | Arabidopsis thaliana |
2.1.1.275 | ? | x * 41500, calculated from sequence | Arabidopsis thaliana |
2.1.1.276 | ? | x * 43300, calculated from sequence | Arabidopsis thaliana |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.275 | GAMT1 | - |
Arabidopsis thaliana |
2.1.1.276 | GAMT2 | - |
Arabidopsis thaliana |
2.1.1.276 | gibberellin A4 O-methyltransferase | - |
Arabidopsis thaliana |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.275 | 30 | - |
assay at | Arabidopsis thaliana |
2.1.1.276 | 30 | - |
assay at | Arabidopsis thaliana |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.275 | 0.01 | - |
gibberellin a4 | pH 8.0, 30°C | Arabidopsis thaliana | |
2.1.1.275 | 0.026 | - |
gibberellin a9 | pH 8.0, 30°C | Arabidopsis thaliana | |
2.1.1.276 | 0.0015 | - |
gibberellin a4 | pH 8.0, 30°C | Arabidopsis thaliana | |
2.1.1.276 | 0.0018 | - |
gibberellin a9 | pH 8.0, 30°C | Arabidopsis thaliana |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.275 | 7.5 | - |
- |
Arabidopsis thaliana |
2.1.1.276 | 8 | - |
- |
Arabidopsis thaliana |
EC Number | Organism | Comment | Expression |
---|---|---|---|
2.1.1.275 | Arabidopsis thaliana | expressed mainly in whole siliques, peak transcript levels from the middle until the end of silique development | up |
2.1.1.276 | Arabidopsis thaliana | expressed mainly in whole siliques, peak transcript levels from the middle until the end of silique development | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.1.1.275 | malfunction | GAMT1 null mutant shows reduced inhibition of germination, compared with the wild type, when placed on plates containing the gibberellin biosynthesis inhibitor ancymidol | Arabidopsis thaliana |
2.1.1.275 | physiological function | methylation of gibberellins by the enzyme serves to deactivate gibberellins and initiate their degradation as the seeds mature | Arabidopsis thaliana |
2.1.1.276 | malfunction | GAMT2 null mutant shows reduced inhibition of germination, compared with the wild type, when placed on plates containing the gibberellin biosynthesis inhibitor ancymidol | Arabidopsis thaliana |
2.1.1.276 | physiological function | methylation of gibberellins by the enzyme serves to deactivate gibberellins and initiate their degradation as the seeds mature | Arabidopsis thaliana |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.275 | 1.6 | - |
gibberellin a9 | pH 8.0, 30°C | Arabidopsis thaliana | |
2.1.1.275 | 1.85 | - |
gibberellin a4 | pH 8.0, 30°C | Arabidopsis thaliana | |
2.1.1.276 | 0.2 | - |
gibberellin a4 | pH 8.0, 30°C | Arabidopsis thaliana | |
2.1.1.276 | 0.9 | - |
gibberellin a9 | pH 8.0, 30°C | Arabidopsis thaliana |