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Literature summary extracted from

  • Kendziorek, M.; Paszkowski, A.; Zagdanska, B.
    Biochemical characterization and kinetic properties of alanine aminotransferase homologues partially purified from wheat (Triticum aestivum L.) (2012), Phytochemistry, 82, 7-14.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.2 4-hydroxymercuribenzoate
-
 Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.2 0.19
-
 2-oxoglutarate AlaAT1, pH 7.3, 25°C Triticum aestivum
2.6.1.2 0.22
-
 pyruvate AlaAT1, pH 7.3, 25°C Triticum aestivum
2.6.1.2 0.29
-
 pyruvate AlaAT2, pH 7.3, 25°C Triticum aestivum
2.6.1.2 0.35
-
 2-oxoglutarate AlaAT2, pH 7.3, 25°C Triticum aestivum
2.6.1.2 6.05
-
 L-alanine AlaAT1, pH 7.3, 25°C Triticum aestivum
2.6.1.2 6.25
-
 L-glutamate AlaAT1, pH 7.3, 25°C Triticum aestivum
2.6.1.2 6.66
-
 L-glutamate AlaAT2, pH 7.3, 25°C Triticum aestivum
2.6.1.2 6.9
-
 L-alanine AlaAT2, pH 7.3, 25°C Triticum aestivum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.2 61300
-
 SDS-PAGE Triticum aestivum
2.6.1.2 65000
-
 1 * 65000 Triticum aestivum

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.2 Triticum aestivum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.2 using saline precipitation, gel filtration, preparative electrophoresis and anion exchange chromatography on protein-Pak Q 8HR column attached to HPLC Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.2 seedling
-
 Triticum aestivum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.2 L-alanine + 2-oxoglutarate
-
 Triticum aestivum pyruvate + L-glutamate
-
 ?
2.6.1.2 L-alanine + 2-oxoglutarate
-
 Triticum aestivum pyruvate + L-glutamate
-
 r

Subunits

EC Number Subunits Comment Organism
2.6.1.2 monomer 1 * 65000 Triticum aestivum

Synonyms

EC Number Synonyms Comment Organism
2.6.1.2 AlaAT1
-
 Triticum aestivum
2.6.1.2 AlaAT2
-
 Triticum aestivum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.2 127.7
-
 pyruvate AlaAT1, cosubstrate: L-glutamate, pH 7.3, 25°C Triticum aestivum
2.6.1.2 127.7
-
 L-glutamate AlaAT1, cosubstrate: pyruvate, pH 7.3, 25°C Triticum aestivum
2.6.1.2 134.9
-
 L-alanine AlaAT1, cosubstrate: 2-oxoglutarate, pH 7.3, 25°C Triticum aestivum
2.6.1.2 134.9
-
 2-oxoglutarate AlaAT1, cosubstrate: L-alanine, pH 7.3, 25°C Triticum aestivum
2.6.1.2 136
-
 pyruvate AlaAT2, cosubstrate: L-glutamate, pH 7.3, 25°C Triticum aestivum
2.6.1.2 136
-
 L-glutamate AlaAT2, cosubstrate: pyruvate, pH 7.3, 25°C Triticum aestivum
2.6.1.2 170
-
 L-alanine AlaAT2, cosubstrate: 2-oxoglutarate, pH 7.3, 25°C Triticum aestivum
2.6.1.2 170
-
 2-oxoglutarate AlaAT2, cosubstrate: L-alanine, pH 7.3, 25°C Triticum aestivum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.6.1.2 19630
-
 pyruvate AlaAT1, cosubstrate: L-glutamate, pH 7.3, 25°C Triticum aestivum
2.6.1.2 19630
-
 L-glutamate AlaAT1, cosubstrate: pyruvate, pH 7.3, 25°C Triticum aestivum
2.6.1.2 20510
-
 pyruvate AlaAT2, cosubstrate: L-glutamate, pH 7.3, 25°C Triticum aestivum
2.6.1.2 20510
-
 L-glutamate AlaAT2, cosubstrate: pyruvate, pH 7.3, 25°C Triticum aestivum
2.6.1.2 22290
-
 L-alanine AlaAT1, cosubstrate: 2-oxoglutarate, pH 7.3, 25°C Triticum aestivum
2.6.1.2 22290
-
 2-oxoglutarate AlaAT1, cosubstrate: L-alanine, pH 7.3, 25°C Triticum aestivum
2.6.1.2 24640
-
 L-alanine AlaAT2, cosubstrate: 2-oxoglutarate, pH 7.3, 25°C Triticum aestivum
2.6.1.2 24640
-
 2-oxoglutarate AlaAT2, cosubstrate: L-alanine, pH 7.3, 25°C Triticum aestivum